Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health
Given their coastal site fidelity and opportunistic foraging behavior, harbor seals (Phoca vitulina) may serve as sentinels for coastal ecosystem health. Seals using urbanized coastal habitat can acquire enteric bacteria, including Vibrio, that may affect their health. To understand Vibrio dynamics...
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ftsanjosestate:oai:scholarworks.sjsu.edu:etd_theses-7782 2023-07-30T04:06:21+02:00 Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health Hughes, Stephanie Nichole 2012-01-01T08:00:00Z application/pdf https://scholarworks.sjsu.edu/etd_theses/4235 https://doi.org/10.31979/etd.5wc5-enkg https://scholarworks.sjsu.edu/context/etd_theses/article/7782/viewcontent/Hughes_s.PDF unknown SJSU ScholarWorks https://scholarworks.sjsu.edu/etd_theses/4235 doi:10.31979/etd.5wc5-enkg https://scholarworks.sjsu.edu/context/etd_theses/article/7782/viewcontent/Hughes_s.PDF Master's Theses bacterial indicator harbor seals marine mammal health Vibrio virulence Ecology Microbiology Veterinary medicine text 2012 ftsanjosestate https://doi.org/10.31979/etd.5wc5-enkg 2023-07-17T18:37:09Z Given their coastal site fidelity and opportunistic foraging behavior, harbor seals (Phoca vitulina) may serve as sentinels for coastal ecosystem health. Seals using urbanized coastal habitat can acquire enteric bacteria, including Vibrio, that may affect their health. To understand Vibrio dynamics in seals, demographic and environmental factors were tested for predicting potentially virulent Vibrio in free-ranging and stranded Pacific harbor seals (P. v. richardii) off the coast of California. Vibrio prevalence did not vary with season and was greater in free-ranging seals (29%, n = 319) compared with stranded seals (17%, n = 189). Of the factors tested, location, turbidity, and/or salinity best predicted Vibrio prevalence in free-ranging seals. The relationship of environmental factors with Vibrio prevalence differed by location and may be related to oceanographic or terrestrial contributions to water quality. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae were observed in seals with V. cholerae found almost exclusively in stranded pups and yearlings. Additionally, virulence genes (trh and tdh) were detected in Vibrio parahaemolyticus isolates. Vibrio cholerae isolates lacked targeted virulence genes, but were hemolytic. Three out of four stranded pups with V. parahaemolyticus (trh+, and/or tdh+) died in rehabilitation, but the role of Vibrio in causing mortality is unclear, and Vibrio expression of virulence genes should be investigated. Considering that humans share the environment and food resources with seals, potentially virulent Vibrio observed in seals also may be of concern to human health. Text Phoca vitulina San José State University: SJSU ScholarWorks Pacific |
institution |
Open Polar |
collection |
San José State University: SJSU ScholarWorks |
op_collection_id |
ftsanjosestate |
language |
unknown |
topic |
bacterial indicator harbor seals marine mammal health Vibrio virulence Ecology Microbiology Veterinary medicine |
spellingShingle |
bacterial indicator harbor seals marine mammal health Vibrio virulence Ecology Microbiology Veterinary medicine Hughes, Stephanie Nichole Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health |
topic_facet |
bacterial indicator harbor seals marine mammal health Vibrio virulence Ecology Microbiology Veterinary medicine |
description |
Given their coastal site fidelity and opportunistic foraging behavior, harbor seals (Phoca vitulina) may serve as sentinels for coastal ecosystem health. Seals using urbanized coastal habitat can acquire enteric bacteria, including Vibrio, that may affect their health. To understand Vibrio dynamics in seals, demographic and environmental factors were tested for predicting potentially virulent Vibrio in free-ranging and stranded Pacific harbor seals (P. v. richardii) off the coast of California. Vibrio prevalence did not vary with season and was greater in free-ranging seals (29%, n = 319) compared with stranded seals (17%, n = 189). Of the factors tested, location, turbidity, and/or salinity best predicted Vibrio prevalence in free-ranging seals. The relationship of environmental factors with Vibrio prevalence differed by location and may be related to oceanographic or terrestrial contributions to water quality. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae were observed in seals with V. cholerae found almost exclusively in stranded pups and yearlings. Additionally, virulence genes (trh and tdh) were detected in Vibrio parahaemolyticus isolates. Vibrio cholerae isolates lacked targeted virulence genes, but were hemolytic. Three out of four stranded pups with V. parahaemolyticus (trh+, and/or tdh+) died in rehabilitation, but the role of Vibrio in causing mortality is unclear, and Vibrio expression of virulence genes should be investigated. Considering that humans share the environment and food resources with seals, potentially virulent Vibrio observed in seals also may be of concern to human health. |
format |
Text |
author |
Hughes, Stephanie Nichole |
author_facet |
Hughes, Stephanie Nichole |
author_sort |
Hughes, Stephanie Nichole |
title |
Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health |
title_short |
Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health |
title_full |
Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health |
title_fullStr |
Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health |
title_full_unstemmed |
Dynamics of Vibrio with virulence genes detected in Pacific harbor seals ( Phoca vitulina richardii ) off California: implications for marine mammal health |
title_sort |
dynamics of vibrio with virulence genes detected in pacific harbor seals ( phoca vitulina richardii ) off california: implications for marine mammal health |
publisher |
SJSU ScholarWorks |
publishDate |
2012 |
url |
https://scholarworks.sjsu.edu/etd_theses/4235 https://doi.org/10.31979/etd.5wc5-enkg https://scholarworks.sjsu.edu/context/etd_theses/article/7782/viewcontent/Hughes_s.PDF |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Master's Theses |
op_relation |
https://scholarworks.sjsu.edu/etd_theses/4235 doi:10.31979/etd.5wc5-enkg https://scholarworks.sjsu.edu/context/etd_theses/article/7782/viewcontent/Hughes_s.PDF |
op_doi |
https://doi.org/10.31979/etd.5wc5-enkg |
_version_ |
1772818896458350592 |