A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments
As the oceans acidify due to increasing atmospheric CO2, there is a growing need to understand the impact of this process on marine organisms. Field observations are difficult because of multiple covarying factors (e.g., temperature, salinity). As such, there is interest in conducting controlled, la...
Published in: | Limnology and Oceanography: Methods |
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ftrwuniv:oai:docs.rwu.edu:fcas_fp-1812 2023-05-15T17:50:16+02:00 A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments Wilcox-Freeburg, Eric Rhyne, Andrew Robinson, William E. Tlusty, Michael Bourque, Bradford Hannigan, Robyn E. 2013-09-01T07:00:00Z https://docs.rwu.edu/fcas_fp/803 https://doi.org/10.4319/lom.2013.11.485 unknown DOCS@RWU https://docs.rwu.edu/fcas_fp/803 https://doi.org/10.4319/lom.2013.11.485 Arts & Sciences Faculty Publications Marine and Biological Research text 2013 ftrwuniv https://doi.org/10.4319/lom.2013.11.485 2022-07-30T22:35:35Z As the oceans acidify due to increasing atmospheric CO2, there is a growing need to understand the impact of this process on marine organisms. Field observations are difficult because of multiple covarying factors (e.g., temperature, salinity). As such, there is interest in conducting controlled, laboratory experiments to best understand how changes in acidity will affect marine organisms. We tested two intermittent CO2 dosing systems, a "home aquarium hobby" grade pH controller and an industrial process control platform. We assessed stability, accuracy, and precision over 7-d experimental periods as well as relative cost of the two configurations. We also compared three laboratory-grade pH electrodes to the hobbyist electrode to further evaluate electrode quality on systemcontrolled pH stability and drift. Whereas the industrial system offered some benefit with regard to autonomy, our results show that the low-cost hobbyist system can be modified appropriately to provide comparable pH control. We provide a detailed list of procedures and software developed for the implementation of a cost-effective, precision-controlled CO2 dosing system to support laboratory-based ocean acidification experiments. Text Ocean acidification Roger Williams University: DOCS@RWU Limnology and Oceanography: Methods 11 9 485 494 |
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Open Polar |
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Roger Williams University: DOCS@RWU |
op_collection_id |
ftrwuniv |
language |
unknown |
topic |
Marine and Biological Research |
spellingShingle |
Marine and Biological Research Wilcox-Freeburg, Eric Rhyne, Andrew Robinson, William E. Tlusty, Michael Bourque, Bradford Hannigan, Robyn E. A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments |
topic_facet |
Marine and Biological Research |
description |
As the oceans acidify due to increasing atmospheric CO2, there is a growing need to understand the impact of this process on marine organisms. Field observations are difficult because of multiple covarying factors (e.g., temperature, salinity). As such, there is interest in conducting controlled, laboratory experiments to best understand how changes in acidity will affect marine organisms. We tested two intermittent CO2 dosing systems, a "home aquarium hobby" grade pH controller and an industrial process control platform. We assessed stability, accuracy, and precision over 7-d experimental periods as well as relative cost of the two configurations. We also compared three laboratory-grade pH electrodes to the hobbyist electrode to further evaluate electrode quality on systemcontrolled pH stability and drift. Whereas the industrial system offered some benefit with regard to autonomy, our results show that the low-cost hobbyist system can be modified appropriately to provide comparable pH control. We provide a detailed list of procedures and software developed for the implementation of a cost-effective, precision-controlled CO2 dosing system to support laboratory-based ocean acidification experiments. |
format |
Text |
author |
Wilcox-Freeburg, Eric Rhyne, Andrew Robinson, William E. Tlusty, Michael Bourque, Bradford Hannigan, Robyn E. |
author_facet |
Wilcox-Freeburg, Eric Rhyne, Andrew Robinson, William E. Tlusty, Michael Bourque, Bradford Hannigan, Robyn E. |
author_sort |
Wilcox-Freeburg, Eric |
title |
A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments |
title_short |
A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments |
title_full |
A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments |
title_fullStr |
A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments |
title_full_unstemmed |
A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments |
title_sort |
comparison of two ph-stat carbon dioxide dosing systems for ocean acidification experiments |
publisher |
DOCS@RWU |
publishDate |
2013 |
url |
https://docs.rwu.edu/fcas_fp/803 https://doi.org/10.4319/lom.2013.11.485 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Arts & Sciences Faculty Publications |
op_relation |
https://docs.rwu.edu/fcas_fp/803 https://doi.org/10.4319/lom.2013.11.485 |
op_doi |
https://doi.org/10.4319/lom.2013.11.485 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
11 |
container_issue |
9 |
container_start_page |
485 |
op_container_end_page |
494 |
_version_ |
1766156951013556224 |