Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations:
Iron has been widely recognized as a potentially key factor in promoting nitrogen fixation by Trichodesmium. Data from both laboratory and field studies demonstrates that increasing the iron concentrations stimulates growth, photosynthetic rates and nitrogen fixation of both cultured and natural pop...
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ftrutgersuniv:oai:example.org:rutgers-lib:25069 2023-05-15T17:33:37+02:00 Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations: Whittaker, Sherrie Whittaker, Sherrie (author) Falkowski, Paul (chair) Schofield, Oscar (internal member) Bidle, Kay (internal member) Rutgers University Graduate School - New Brunswick 2008 viii, 32 p. : ill. electronic resource application/pdf http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000050470 eng eng Rutgers University Electronic Theses and Dissertations Graduate School - New Brunswick Electronic Theses and Dissertations rucore19991600001 http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000050470 Oceanography Trichodesmium Nitrogenase Text theses 2008 ftrutgersuniv 2022-05-30T13:37:21Z Iron has been widely recognized as a potentially key factor in promoting nitrogen fixation by Trichodesmium. Data from both laboratory and field studies demonstrates that increasing the iron concentrations stimulates growth, photosynthetic rates and nitrogen fixation of both cultured and natural populations. However, quantitative studies that elucidate relationships between cellular iron quotas and physiological mechanisms have been limited. In this study, molecular techniques enabled quantification of the amount of nitrogenase expressed in iron-replete cultures of Trichodesmium IMS 101 over a diel cycle. A standard of the purified iron component of nitrogenase was generated from an expression and protein purification system. Using this standard and known values of intracellular carbon, the amount of nitrogenase per carbon at peak expression was measured, 0.038 mg nitrogenase: mg C. The quantity of iron bound in the nitrogenase structure was then calculated; Fe:C equal to 236.53 [mu]mol: mol. Using estimates of Trichodesmium biomass from the literature, the amount of iron bound in the nitrogenase structure was calculated for various ocean regions, resulting in 2.22 [mu]mol m-3 and 0.05 [mu]mol m-3 of iron bound in nitrogenase in the subtropical North Atlantic and North Pacific, respectively. M.S. Includes bibliographical references (p. 29-32) by Sherrie Whittaker Thesis North Atlantic RUcore - Rutgers University Community Repository Pacific |
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English |
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Oceanography Trichodesmium Nitrogenase |
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Oceanography Trichodesmium Nitrogenase Whittaker, Sherrie Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations: |
topic_facet |
Oceanography Trichodesmium Nitrogenase |
description |
Iron has been widely recognized as a potentially key factor in promoting nitrogen fixation by Trichodesmium. Data from both laboratory and field studies demonstrates that increasing the iron concentrations stimulates growth, photosynthetic rates and nitrogen fixation of both cultured and natural populations. However, quantitative studies that elucidate relationships between cellular iron quotas and physiological mechanisms have been limited. In this study, molecular techniques enabled quantification of the amount of nitrogenase expressed in iron-replete cultures of Trichodesmium IMS 101 over a diel cycle. A standard of the purified iron component of nitrogenase was generated from an expression and protein purification system. Using this standard and known values of intracellular carbon, the amount of nitrogenase per carbon at peak expression was measured, 0.038 mg nitrogenase: mg C. The quantity of iron bound in the nitrogenase structure was then calculated; Fe:C equal to 236.53 [mu]mol: mol. Using estimates of Trichodesmium biomass from the literature, the amount of iron bound in the nitrogenase structure was calculated for various ocean regions, resulting in 2.22 [mu]mol m-3 and 0.05 [mu]mol m-3 of iron bound in nitrogenase in the subtropical North Atlantic and North Pacific, respectively. M.S. Includes bibliographical references (p. 29-32) by Sherrie Whittaker |
author2 |
Whittaker, Sherrie (author) Falkowski, Paul (chair) Schofield, Oscar (internal member) Bidle, Kay (internal member) Rutgers University Graduate School - New Brunswick |
format |
Thesis |
author |
Whittaker, Sherrie |
author_facet |
Whittaker, Sherrie |
author_sort |
Whittaker, Sherrie |
title |
Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations: |
title_short |
Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations: |
title_full |
Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations: |
title_fullStr |
Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations: |
title_full_unstemmed |
Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations: |
title_sort |
using molecular techniques to quantify iron bound nitrogenase in trichodesmium ims 101 and natural populations: |
publishDate |
2008 |
url |
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000050470 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Rutgers University Electronic Theses and Dissertations Graduate School - New Brunswick Electronic Theses and Dissertations rucore19991600001 http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000050470 |
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1766132178200035328 |