Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions.
Standing genetic variation is important for population persistence in extreme environmental conditions. While some species may have the capacity to adapt to predicted average future global change conditions, the ability to survive extreme events is largely unknown. We used single-generation selectio...
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ftroysocietyfig:oai:figshare.com:article/8186948 2023-05-15T17:51:28+02:00 Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions. Reid S. Brennan April D. Garrett Kaitlin E. Huber Heidi Hargarten Melissa H. Pespeni 2019-06-10T09:26:58Z https://doi.org/10.6084/m9.figshare.8186948.v2 https://figshare.com/articles/journal_contribution/Supplemental_Methods_and_Figures_from_Rare_genetic_variation_and_balanced_polymorphisms_are_important_for_survival_in_global_change_conditions/8186948 unknown doi:10.6084/m9.figshare.8186948.v2 https://figshare.com/articles/journal_contribution/Supplemental_Methods_and_Figures_from_Rare_genetic_variation_and_balanced_polymorphisms_are_important_for_survival_in_global_change_conditions/8186948 CC BY 4.0 CC-BY Evolutionary Biology Genomics standing genetic variation ocean acidification adaptation experimental selection select and resequence extreme events capture sequencing Text Journal contribution 2019 ftroysocietyfig https://doi.org/10.6084/m9.figshare.8186948.v2 2022-01-01T19:20:16Z Standing genetic variation is important for population persistence in extreme environmental conditions. While some species may have the capacity to adapt to predicted average future global change conditions, the ability to survive extreme events is largely unknown. We used single-generation selection experiments on hundreds of thousands of Strongylocentrotus purpuratus sea urchin larvae generated from wild-caught adults to identify adaptive genetic variation responsive to moderate (pH 8.0) and extreme (pH 7.5) low-pH conditions. Sequencing genomic DNA from pools of larvae, we identified consistent changes in allele frequencies across replicate cultures for each pH condition and observed increased linkage disequilibrium around selected loci, revealing selection on recombined standing genetic variation. We found that loci responding uniquely to either selection regime were at low starting allele frequencies while variants that responded to both pH conditions (11.6% of selected variants) started at high frequencies. Loci under selection performed functions related to energetics, pH tolerance, cell growth and actin/cytoskeleton dynamics. These results highlight that persistence in future conditions will require two classes of genetic variation: common, pH-responsive variants maintained by balancing selection in a heterogeneous environment, and rare variants, particularly for extreme conditions, that must be maintained by large population sizes. Other Non-Article Part of Journal/Newspaper Ocean acidification The Royal Society: Figshare |
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Open Polar |
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The Royal Society: Figshare |
op_collection_id |
ftroysocietyfig |
language |
unknown |
topic |
Evolutionary Biology Genomics standing genetic variation ocean acidification adaptation experimental selection select and resequence extreme events capture sequencing |
spellingShingle |
Evolutionary Biology Genomics standing genetic variation ocean acidification adaptation experimental selection select and resequence extreme events capture sequencing Reid S. Brennan April D. Garrett Kaitlin E. Huber Heidi Hargarten Melissa H. Pespeni Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions. |
topic_facet |
Evolutionary Biology Genomics standing genetic variation ocean acidification adaptation experimental selection select and resequence extreme events capture sequencing |
description |
Standing genetic variation is important for population persistence in extreme environmental conditions. While some species may have the capacity to adapt to predicted average future global change conditions, the ability to survive extreme events is largely unknown. We used single-generation selection experiments on hundreds of thousands of Strongylocentrotus purpuratus sea urchin larvae generated from wild-caught adults to identify adaptive genetic variation responsive to moderate (pH 8.0) and extreme (pH 7.5) low-pH conditions. Sequencing genomic DNA from pools of larvae, we identified consistent changes in allele frequencies across replicate cultures for each pH condition and observed increased linkage disequilibrium around selected loci, revealing selection on recombined standing genetic variation. We found that loci responding uniquely to either selection regime were at low starting allele frequencies while variants that responded to both pH conditions (11.6% of selected variants) started at high frequencies. Loci under selection performed functions related to energetics, pH tolerance, cell growth and actin/cytoskeleton dynamics. These results highlight that persistence in future conditions will require two classes of genetic variation: common, pH-responsive variants maintained by balancing selection in a heterogeneous environment, and rare variants, particularly for extreme conditions, that must be maintained by large population sizes. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Reid S. Brennan April D. Garrett Kaitlin E. Huber Heidi Hargarten Melissa H. Pespeni |
author_facet |
Reid S. Brennan April D. Garrett Kaitlin E. Huber Heidi Hargarten Melissa H. Pespeni |
author_sort |
Reid S. Brennan |
title |
Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions. |
title_short |
Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions. |
title_full |
Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions. |
title_fullStr |
Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions. |
title_full_unstemmed |
Supplemental Methods, Figures and Table S1 from Rare genetic variation and balanced polymorphisms are important for survival in global change conditions. |
title_sort |
supplemental methods, figures and table s1 from rare genetic variation and balanced polymorphisms are important for survival in global change conditions. |
publishDate |
2019 |
url |
https://doi.org/10.6084/m9.figshare.8186948.v2 https://figshare.com/articles/journal_contribution/Supplemental_Methods_and_Figures_from_Rare_genetic_variation_and_balanced_polymorphisms_are_important_for_survival_in_global_change_conditions/8186948 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
doi:10.6084/m9.figshare.8186948.v2 https://figshare.com/articles/journal_contribution/Supplemental_Methods_and_Figures_from_Rare_genetic_variation_and_balanced_polymorphisms_are_important_for_survival_in_global_change_conditions/8186948 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.8186948.v2 |
_version_ |
1766158625804386304 |