Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens
Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered res...
Published in: | Scientific Reports |
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Robert Koch-Institut, Infektionskrankheiten / Erreger
2018
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Online Access: | http://edoc.rki.de/oa/articles/remirLBRU0kY2/PDF/20uztsQBDScAE.pdf http://edoc.rki.de/176904/3004 https://nbn-resolving.org/urn:nbn:de:0257-10057856 https://doi.org/10.1038/s41598-018-21143-0 https://doi.org/10.25646/2929 |
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ftrobertkoch:oai:edoc.rki.de:176904/3004 2023-05-15T17:12:35+02:00 Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank 2018-02-12 application/pdf http://edoc.rki.de/oa/articles/remirLBRU0kY2/PDF/20uztsQBDScAE.pdf http://edoc.rki.de/176904/3004 https://nbn-resolving.org/urn:nbn:de:0257-10057856 https://doi.org/10.1038/s41598-018-21143-0 https://doi.org/10.25646/2929 eng eng Robert Koch-Institut, Infektionskrankheiten / Erreger http://edoc.rki.de/oa/articles/remirLBRU0kY2/PDF/20uztsQBDScAE.pdf http://edoc.rki.de/176904/3004 urn:nbn:de:0257-10057856 doi:10.1038/s41598-018-21143-0 http://dx.doi.org/10.25646/2929 610 Medizin ddc:610 periodicalPart doc-type:periodicalPart 2018 ftrobertkoch https://doi.org/10.1038/s41598-018-21143-0 https://doi.org/10.25646/2929 2022-06-20T05:48:38Z Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered research in these animal species. Here we report the recombinant production and functional characterization of IFN-γ, a central mediator of host’s innate and adaptive immune responses, from the bank vole M. glareolus. Soluble dimeric recMgIFN-γ was purified in high yield from Escherichia coli. Its activity on M. glareolus and Microtus arvalis kidney cell lines was assessed by immunofluorescent detection of nuclear translocation and phosphorylation of the transcription factor STAT1. RecMgIFN-γ also induced expression of an IFN-γ-regulated innate immunity gene. Inhibition of vesicular stomatitis virus replication in vole cells upon recMgIFN-γ treatment provided further evidence of its biological activity. Finally, we established a recMgIFN-γ-responsive bank vole reporter cell line that allows the sensitive titration of the cytokine activity via a bioluminescence reporter assay. Taken together, we report valuable tools for future investigations on the immune response against zoonotic pathogens in their natural animal hosts, which might foster the development of novel animal models. Other Non-Article Part of Journal/Newspaper Microtus arvalis Robert Koch Institute: Publications Scientific Reports 8 1 |
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Robert Koch Institute: Publications |
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ftrobertkoch |
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610 Medizin ddc:610 |
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610 Medizin ddc:610 Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
topic_facet |
610 Medizin ddc:610 |
description |
Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered research in these animal species. Here we report the recombinant production and functional characterization of IFN-γ, a central mediator of host’s innate and adaptive immune responses, from the bank vole M. glareolus. Soluble dimeric recMgIFN-γ was purified in high yield from Escherichia coli. Its activity on M. glareolus and Microtus arvalis kidney cell lines was assessed by immunofluorescent detection of nuclear translocation and phosphorylation of the transcription factor STAT1. RecMgIFN-γ also induced expression of an IFN-γ-regulated innate immunity gene. Inhibition of vesicular stomatitis virus replication in vole cells upon recMgIFN-γ treatment provided further evidence of its biological activity. Finally, we established a recMgIFN-γ-responsive bank vole reporter cell line that allows the sensitive titration of the cytokine activity via a bioluminescence reporter assay. Taken together, we report valuable tools for future investigations on the immune response against zoonotic pathogens in their natural animal hosts, which might foster the development of novel animal models. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank |
author_facet |
Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank |
author_sort |
Torelli, Francesca |
title |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_short |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_full |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_fullStr |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_full_unstemmed |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_sort |
recombinant ifn-γ from the bank vole myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
publisher |
Robert Koch-Institut, Infektionskrankheiten / Erreger |
publishDate |
2018 |
url |
http://edoc.rki.de/oa/articles/remirLBRU0kY2/PDF/20uztsQBDScAE.pdf http://edoc.rki.de/176904/3004 https://nbn-resolving.org/urn:nbn:de:0257-10057856 https://doi.org/10.1038/s41598-018-21143-0 https://doi.org/10.25646/2929 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
http://edoc.rki.de/oa/articles/remirLBRU0kY2/PDF/20uztsQBDScAE.pdf http://edoc.rki.de/176904/3004 urn:nbn:de:0257-10057856 doi:10.1038/s41598-018-21143-0 http://dx.doi.org/10.25646/2929 |
op_doi |
https://doi.org/10.1038/s41598-018-21143-0 https://doi.org/10.25646/2929 |
container_title |
Scientific Reports |
container_volume |
8 |
container_issue |
1 |
_version_ |
1766069371946401792 |