T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro
International audience The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the im...
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Online Access: | https://riip.hal.science/pasteur-01855960 https://doi.org/10.1016/j.marpolbul.2017.12.001 |
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ftriip:oai:HAL:pasteur-01855960v1 2024-06-23T07:53:29+00:00 T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammill, Mike, O. de Guise, Sylvain Fournier, Michel Institut Armand Frappier (INRS-IAF) Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP) Fisheries and Oceans Canada (DFO) University of Connecticut (UCONN) This work was supported by the Canada Research Chair in Environmental Immunotoxicity (207463) (MF) and funded in part by the Department of Fisheries and Oceans (MH). 2018-02 https://riip.hal.science/pasteur-01855960 https://doi.org/10.1016/j.marpolbul.2017.12.001 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marpolbul.2017.12.001 info:eu-repo/semantics/altIdentifier/pmid/29475659 pasteur-01855960 https://riip.hal.science/pasteur-01855960 doi:10.1016/j.marpolbul.2017.12.001 PUBMED: 29475659 ISSN: 0025-326X EISSN: 1879-3363 Marine Pollution Bulletin https://riip.hal.science/pasteur-01855960 Marine Pollution Bulletin, 2018, 127, pp.225 - 234. ⟨10.1016/j.marpolbul.2017.12.001⟩ Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina MESH: Animals MESH: Carbamazepine MESH: T-Lymphocytes MESH: Water Pollutants Chemical MESH: Cell Proliferation MESH: Erythromycin MESH: Ethinyl Estradiol MESH: Female MESH: Leukocytes Mononuclear MESH: Male MESH: Naproxen MESH: Phoca [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2018 ftriip https://doi.org/10.1016/j.marpolbul.2017.12.001 2024-05-27T23:55:54Z International audience The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals. Article in Journal/Newspaper harbor seal Phoca vitulina Réseau International des Instituts Pasteur, Paris: HAL-RIIP Marine Pollution Bulletin 127 225 234 |
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Open Polar |
collection |
Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
op_collection_id |
ftriip |
language |
English |
topic |
Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina MESH: Animals MESH: Carbamazepine MESH: T-Lymphocytes MESH: Water Pollutants Chemical MESH: Cell Proliferation MESH: Erythromycin MESH: Ethinyl Estradiol MESH: Female MESH: Leukocytes Mononuclear MESH: Male MESH: Naproxen MESH: Phoca [SDV]Life Sciences [q-bio] |
spellingShingle |
Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina MESH: Animals MESH: Carbamazepine MESH: T-Lymphocytes MESH: Water Pollutants Chemical MESH: Cell Proliferation MESH: Erythromycin MESH: Ethinyl Estradiol MESH: Female MESH: Leukocytes Mononuclear MESH: Male MESH: Naproxen MESH: Phoca [SDV]Life Sciences [q-bio] Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammill, Mike, O. de Guise, Sylvain Fournier, Michel T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
topic_facet |
Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina MESH: Animals MESH: Carbamazepine MESH: T-Lymphocytes MESH: Water Pollutants Chemical MESH: Cell Proliferation MESH: Erythromycin MESH: Ethinyl Estradiol MESH: Female MESH: Leukocytes Mononuclear MESH: Male MESH: Naproxen MESH: Phoca [SDV]Life Sciences [q-bio] |
description |
International audience The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals. |
author2 |
Institut Armand Frappier (INRS-IAF) Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP) Fisheries and Oceans Canada (DFO) University of Connecticut (UCONN) This work was supported by the Canada Research Chair in Environmental Immunotoxicity (207463) (MF) and funded in part by the Department of Fisheries and Oceans (MH). |
format |
Article in Journal/Newspaper |
author |
Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammill, Mike, O. de Guise, Sylvain Fournier, Michel |
author_facet |
Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammill, Mike, O. de Guise, Sylvain Fournier, Michel |
author_sort |
Kleinert, Christine |
title |
T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_short |
T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_full |
T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_fullStr |
T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_full_unstemmed |
T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_sort |
t lymphocyte-proliferative responses of harbor seal ( phoca vitulina ) peripheral blood mononuclear cells (pbmcs) exposed to pharmaceuticals in vitro |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://riip.hal.science/pasteur-01855960 https://doi.org/10.1016/j.marpolbul.2017.12.001 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
ISSN: 0025-326X EISSN: 1879-3363 Marine Pollution Bulletin https://riip.hal.science/pasteur-01855960 Marine Pollution Bulletin, 2018, 127, pp.225 - 234. ⟨10.1016/j.marpolbul.2017.12.001⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marpolbul.2017.12.001 info:eu-repo/semantics/altIdentifier/pmid/29475659 pasteur-01855960 https://riip.hal.science/pasteur-01855960 doi:10.1016/j.marpolbul.2017.12.001 PUBMED: 29475659 |
op_doi |
https://doi.org/10.1016/j.marpolbul.2017.12.001 |
container_title |
Marine Pollution Bulletin |
container_volume |
127 |
container_start_page |
225 |
op_container_end_page |
234 |
_version_ |
1802645186065465344 |