T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro

International audience The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the im...

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Bibliographic Details
Published in:Marine Pollution Bulletin
Main Authors: Kleinert, Christine, Lacaze, Emilie, Fortier, Marlène, Hammill, Mike, O., de Guise, Sylvain, Fournier, Michel
Other Authors: Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP), Fisheries and Oceans Canada (DFO), University of Connecticut (UCONN), This work was supported by the Canada Research Chair in Environmental Immunotoxicity (207463) (MF) and funded in part by the Department of Fisheries and Oceans (MH).
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2018
Subjects:
Immunotoxicity
Marine mammal
Pharmaceuticals
Phoca vitulina
MESH: Animals
MESH: Carbamazepine
MESH: T-Lymphocytes
MESH: Water Pollutants
Chemical
MESH: Cell Proliferation
MESH: Erythromycin
MESH: Ethinyl Estradiol
MESH: Female
MESH: Leukocytes
Mononuclear
MESH: Male
MESH: Naproxen
MESH: Phoca
[SDV]Life Sciences [q-bio]
harbor seal
Online Access:https://riip.hal.science/pasteur-01855960
https://doi.org/10.1016/j.marpolbul.2017.12.001
Description
Summary:International audience The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals.

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