Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line.
International audience The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant redu...
Published in: | Marine Pollution Bulletin |
---|---|
Main Authors: | , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2017
|
Subjects: | |
Online Access: | https://riip.hal.science/pasteur-01534638 https://doi.org/10.1016/j.marpolbul.2017.02.041 |
id |
ftriip:oai:HAL:pasteur-01534638v1 |
---|---|
record_format |
openpolar |
spelling |
ftriip:oai:HAL:pasteur-01534638v1 2024-06-23T07:53:29+00:00 Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line. Kleinert, Christine Lacaze, Emilie Mounier, Méryl de Guise, Sylvain Fournier, Michel Institut Armand Frappier (INRS-IAF) Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP) Department of Pathobiology and Veterinary Science University of Connecticut (UCONN) 2017-05-15 https://riip.hal.science/pasteur-01534638 https://doi.org/10.1016/j.marpolbul.2017.02.041 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marpolbul.2017.02.041 info:eu-repo/semantics/altIdentifier/pmid/28262249 pasteur-01534638 https://riip.hal.science/pasteur-01534638 doi:10.1016/j.marpolbul.2017.02.041 PUBMED: 28262249 ISSN: 0025-326X EISSN: 1879-3363 Marine Pollution Bulletin https://riip.hal.science/pasteur-01534638 Marine Pollution Bulletin, 2017, 118 (1-2), pp.237-247. ⟨10.1016/j.marpolbul.2017.02.041⟩ Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2017 ftriip https://doi.org/10.1016/j.marpolbul.2017.02.041 2024-05-27T23:55:54Z International audience The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500μg/L 17α-ethinyl estradiol and 25,000μg/L naproxen. Exposure to 12,500μg/L 17α-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds. Article in Journal/Newspaper harbor seal Phoca vitulina Réseau International des Instituts Pasteur, Paris: HAL-RIIP Marine Pollution Bulletin 118 1-2 237 247 |
institution |
Open Polar |
collection |
Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
op_collection_id |
ftriip |
language |
English |
topic |
Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals [SDV]Life Sciences [q-bio] |
spellingShingle |
Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals [SDV]Life Sciences [q-bio] Kleinert, Christine Lacaze, Emilie Mounier, Méryl de Guise, Sylvain Fournier, Michel Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line. |
topic_facet |
Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals [SDV]Life Sciences [q-bio] |
description |
International audience The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500μg/L 17α-ethinyl estradiol and 25,000μg/L naproxen. Exposure to 12,500μg/L 17α-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds. |
author2 |
Institut Armand Frappier (INRS-IAF) Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP) Department of Pathobiology and Veterinary Science University of Connecticut (UCONN) |
format |
Article in Journal/Newspaper |
author |
Kleinert, Christine Lacaze, Emilie Mounier, Méryl de Guise, Sylvain Fournier, Michel |
author_facet |
Kleinert, Christine Lacaze, Emilie Mounier, Méryl de Guise, Sylvain Fournier, Michel |
author_sort |
Kleinert, Christine |
title |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line. |
title_short |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line. |
title_full |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line. |
title_fullStr |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line. |
title_full_unstemmed |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line. |
title_sort |
immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (phoca vitulina) b lymphoma cell line. |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://riip.hal.science/pasteur-01534638 https://doi.org/10.1016/j.marpolbul.2017.02.041 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
ISSN: 0025-326X EISSN: 1879-3363 Marine Pollution Bulletin https://riip.hal.science/pasteur-01534638 Marine Pollution Bulletin, 2017, 118 (1-2), pp.237-247. ⟨10.1016/j.marpolbul.2017.02.041⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marpolbul.2017.02.041 info:eu-repo/semantics/altIdentifier/pmid/28262249 pasteur-01534638 https://riip.hal.science/pasteur-01534638 doi:10.1016/j.marpolbul.2017.02.041 PUBMED: 28262249 |
op_doi |
https://doi.org/10.1016/j.marpolbul.2017.02.041 |
container_title |
Marine Pollution Bulletin |
container_volume |
118 |
container_issue |
1-2 |
container_start_page |
237 |
op_container_end_page |
247 |
_version_ |
1802645185656520704 |