Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence?
International audience BACKGROUND: Plague is endemic within the central highlands of Madagascar, where its main reservoir is the black rat, Rattus rattus. Typically this species is considered susceptible to plague, rapidly dying after infection inducing the spread of infected fleas and, therefore, d...
Published in: | PLoS ONE |
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Main Authors: | , , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2012
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Online Access: | https://riip.hal.science/pasteur-00835559 https://riip.hal.science/pasteur-00835559/document https://riip.hal.science/pasteur-00835559/file/22719908AndrianaivoarimananaPLoSOne2012_7_6_e38630.pdf https://doi.org/10.1371/journal.pone.0038630 |
id |
ftriip:oai:HAL:pasteur-00835559v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
op_collection_id |
ftriip |
language |
English |
topic |
MESH: Animals Wild MESH: Plague MESH: Rats MESH: Yersinia pestis MESH: Autoantibodies MESH: Disease Reservoirs MESH: Enzyme-Linked Immunosorbent Assay MESH: Female MESH: Immunoglobulin G MESH: Immunoglobulin M MESH: Madagascar MESH: Male [SDV]Life Sciences [q-bio] |
spellingShingle |
MESH: Animals Wild MESH: Plague MESH: Rats MESH: Yersinia pestis MESH: Autoantibodies MESH: Disease Reservoirs MESH: Enzyme-Linked Immunosorbent Assay MESH: Female MESH: Immunoglobulin G MESH: Immunoglobulin M MESH: Madagascar MESH: Male [SDV]Life Sciences [q-bio] Andrianaivoarimanana, Voahangy Telfer, Sandra Rajerison, Minoarisoa Ranjalahy, Michel A Andriamiarimanana, Fehivola Rahaingosoamamitiana, Corinne Rahalison, Lila Jambou, Ronan Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence? |
topic_facet |
MESH: Animals Wild MESH: Plague MESH: Rats MESH: Yersinia pestis MESH: Autoantibodies MESH: Disease Reservoirs MESH: Enzyme-Linked Immunosorbent Assay MESH: Female MESH: Immunoglobulin G MESH: Immunoglobulin M MESH: Madagascar MESH: Male [SDV]Life Sciences [q-bio] |
description |
International audience BACKGROUND: Plague is endemic within the central highlands of Madagascar, where its main reservoir is the black rat, Rattus rattus. Typically this species is considered susceptible to plague, rapidly dying after infection inducing the spread of infected fleas and, therefore, dissemination of the disease to humans. However, persistence of transmission foci in the same area from year to year, supposes mechanisms of maintenance among which rat immune responses could play a major role. Immunity against plague and subsequent rat survival could play an important role in the stabilization of the foci. In this study, we aimed to investigate serological responses to plague in wild black rats from endemic areas of Madagascar. In addition, we evaluate the use of a recently developed rapid serological diagnostic test to investigate the immune response of potential reservoir hosts in plague foci. METHODOLOGY/PRINCIPAL FINDINGS: We experimentally infected wild rats with Yersinia pestis to investigate short and long-term antibody responses. Anti-F1 IgM and IgG were detected to evaluate this antibody response. High levels of anti-F1 IgM and IgG were found in rats one and three weeks respectively after challenge, with responses greatly differing between villages. Plateau in anti-F1 IgM and IgG responses were reached for as few as 500 and 1500 colony forming units (cfu) inoculated respectively. More than 10% of rats were able to maintain anti-F1 responses for more than one year. This anti-F1 response was conveniently followed using dipsticks. CONCLUSION/SIGNIFICANCE: Inoculation of very few bacteria is sufficient to induce high immune response in wild rats, allowing their survival after infection. A great heterogeneity of rat immune responses was found within and between villages which could heavily impact on plague epidemiology. In addition, results indicate that, in the field, anti-F1 dipsticks are efficient to investigate plague outbreaks several months after transmission. |
author2 |
Unité Peste - Plague Unit Antananarivo, Madagascar Institut Pasteur de Madagascar Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) School of Biological Sciences University of Aberdeen Laboratoire Central de la Peste (CNR) This investigation was funded by the Institut Pasteur de Madagascar and the Wellcome Trust (081705/B/06/Z) |
format |
Article in Journal/Newspaper |
author |
Andrianaivoarimanana, Voahangy Telfer, Sandra Rajerison, Minoarisoa Ranjalahy, Michel A Andriamiarimanana, Fehivola Rahaingosoamamitiana, Corinne Rahalison, Lila Jambou, Ronan |
author_facet |
Andrianaivoarimanana, Voahangy Telfer, Sandra Rajerison, Minoarisoa Ranjalahy, Michel A Andriamiarimanana, Fehivola Rahaingosoamamitiana, Corinne Rahalison, Lila Jambou, Ronan |
author_sort |
Andrianaivoarimanana, Voahangy |
title |
Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence? |
title_short |
Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence? |
title_full |
Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence? |
title_fullStr |
Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence? |
title_full_unstemmed |
Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence? |
title_sort |
immune responses to plague infection in wild rattus rattus, in madagascar: a role in foci persistence? |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://riip.hal.science/pasteur-00835559 https://riip.hal.science/pasteur-00835559/document https://riip.hal.science/pasteur-00835559/file/22719908AndrianaivoarimananaPLoSOne2012_7_6_e38630.pdf https://doi.org/10.1371/journal.pone.0038630 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://riip.hal.science/pasteur-00835559 PLoS ONE, 2012, 7 (6), pp.e38630. ⟨10.1371/journal.pone.0038630⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0038630 info:eu-repo/semantics/altIdentifier/pmid/22719908 pasteur-00835559 https://riip.hal.science/pasteur-00835559 https://riip.hal.science/pasteur-00835559/document https://riip.hal.science/pasteur-00835559/file/22719908AndrianaivoarimananaPLoSOne2012_7_6_e38630.pdf doi:10.1371/journal.pone.0038630 PUBMED: 22719908 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0038630 |
container_title |
PLoS ONE |
container_volume |
7 |
container_issue |
6 |
container_start_page |
e38630 |
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1809937281059913728 |
spelling |
ftriip:oai:HAL:pasteur-00835559v1 2024-09-09T20:04:59+00:00 Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence? Andrianaivoarimanana, Voahangy Telfer, Sandra Rajerison, Minoarisoa Ranjalahy, Michel A Andriamiarimanana, Fehivola Rahaingosoamamitiana, Corinne Rahalison, Lila Jambou, Ronan Unité Peste - Plague Unit Antananarivo, Madagascar Institut Pasteur de Madagascar Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) School of Biological Sciences University of Aberdeen Laboratoire Central de la Peste (CNR) This investigation was funded by the Institut Pasteur de Madagascar and the Wellcome Trust (081705/B/06/Z) 2012 https://riip.hal.science/pasteur-00835559 https://riip.hal.science/pasteur-00835559/document https://riip.hal.science/pasteur-00835559/file/22719908AndrianaivoarimananaPLoSOne2012_7_6_e38630.pdf https://doi.org/10.1371/journal.pone.0038630 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0038630 info:eu-repo/semantics/altIdentifier/pmid/22719908 pasteur-00835559 https://riip.hal.science/pasteur-00835559 https://riip.hal.science/pasteur-00835559/document https://riip.hal.science/pasteur-00835559/file/22719908AndrianaivoarimananaPLoSOne2012_7_6_e38630.pdf doi:10.1371/journal.pone.0038630 PUBMED: 22719908 info:eu-repo/semantics/OpenAccess ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://riip.hal.science/pasteur-00835559 PLoS ONE, 2012, 7 (6), pp.e38630. ⟨10.1371/journal.pone.0038630⟩ MESH: Animals Wild MESH: Plague MESH: Rats MESH: Yersinia pestis MESH: Autoantibodies MESH: Disease Reservoirs MESH: Enzyme-Linked Immunosorbent Assay MESH: Female MESH: Immunoglobulin G MESH: Immunoglobulin M MESH: Madagascar MESH: Male [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2012 ftriip https://doi.org/10.1371/journal.pone.0038630 2024-07-22T23:43:40Z International audience BACKGROUND: Plague is endemic within the central highlands of Madagascar, where its main reservoir is the black rat, Rattus rattus. Typically this species is considered susceptible to plague, rapidly dying after infection inducing the spread of infected fleas and, therefore, dissemination of the disease to humans. However, persistence of transmission foci in the same area from year to year, supposes mechanisms of maintenance among which rat immune responses could play a major role. Immunity against plague and subsequent rat survival could play an important role in the stabilization of the foci. In this study, we aimed to investigate serological responses to plague in wild black rats from endemic areas of Madagascar. In addition, we evaluate the use of a recently developed rapid serological diagnostic test to investigate the immune response of potential reservoir hosts in plague foci. METHODOLOGY/PRINCIPAL FINDINGS: We experimentally infected wild rats with Yersinia pestis to investigate short and long-term antibody responses. Anti-F1 IgM and IgG were detected to evaluate this antibody response. High levels of anti-F1 IgM and IgG were found in rats one and three weeks respectively after challenge, with responses greatly differing between villages. Plateau in anti-F1 IgM and IgG responses were reached for as few as 500 and 1500 colony forming units (cfu) inoculated respectively. More than 10% of rats were able to maintain anti-F1 responses for more than one year. This anti-F1 response was conveniently followed using dipsticks. CONCLUSION/SIGNIFICANCE: Inoculation of very few bacteria is sufficient to induce high immune response in wild rats, allowing their survival after infection. A great heterogeneity of rat immune responses was found within and between villages which could heavily impact on plague epidemiology. In addition, results indicate that, in the field, anti-F1 dipsticks are efficient to investigate plague outbreaks several months after transmission. Article in Journal/Newspaper Rattus rattus Réseau International des Instituts Pasteur, Paris: HAL-RIIP PLoS ONE 7 6 e38630 |