Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish
Marine macroalgae (MM) present a great biological potential since they are a source of many essential natural compounds with physiological benefits. Despite this, there is little knowledge about the impact of MM supplementation on protecting fish health from exposure to environmental contaminants. M...
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ftria:oai:ria.ua.pt:10773/41133 2024-04-21T08:13:03+00:00 Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish Proteção de uma dieta rica em macroalgas marinhas contra a acumulação de mercúrio e efeitos bioquímicos e genotóxicos em peixes Neto, Ana Catarina Canastra Kowalski, Patrícia Pereira Cesário, Rute Isabel Telhadas 2026-02-13 http://hdl.handle.net/10773/41133 eng eng http://hdl.handle.net/10773/41133 embargoedAccess https://creativecommons.org/licenses/by/4.0/ Inorganic mercury Marine macroalgae Fish Bioaccumulation Antioxidant and geno-protection Functional foods masterThesis 1483 ftria 2024-03-25T00:30:24Z Marine macroalgae (MM) present a great biological potential since they are a source of many essential natural compounds with physiological benefits. Despite this, there is little knowledge about the impact of MM supplementation on protecting fish health from exposure to environmental contaminants. Mercury (Hg) is well known for its high toxicity, and it can be found at low levels in farm-raised fish. The present dissertation was design in this context, with the aim of addressing a possible protection by a diet supplemented with MM to the juvenile white sea bream (Diplodus sargus), particularly after exposure via water to realistic levels of inorganic Hg (iHg). Thus, the toxicokinetics of iHg was studied, antioxidant protection and neuroprotection as well as anti-genotoxic properties. For this purpose, the fish were fed for 3 months with a MM-enriched diet [total incorporation of 5 %, with the species Fucus vesiculosus, Gracilaria gracilis and Ulva rigida in equal parts - algae-supplemented fish group (AC)], while the non-supplemented fish were fed with a standard feed (SC). Then, both groups on different baseline diets were exposed to realistic levels of iHg (2 μg L-1) via water for 7 days (E7) (AHg and SHg groups), followed by a 14-day post-exposure period (PE14). Control groups, not exposed to iHg, were maintained throughout the experiment (AC and SC). At E7 and PE14, the fish from the different groups (AC, SC, AHg, SHg) were sacrificed and the gills, blood, liver and brain were collected to determine total Hg (tHg) levels, in parallel with the assessment of oxidative stress related endpoints in the gills, liver and brain, where the following biochemical parameters were determined: (i) enzymatic antioxidants [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GR)] and non-enzymatic antioxidants (total glutathione (GSHt)); (ii) damage indicators (lipid peroxidation - LPO); (iii) acetylcholinesterase (AChE) activity, used as an ... Master Thesis White Sea Repositório Institucional da Universidade de Aveiro (RIA) |
institution |
Open Polar |
collection |
Repositório Institucional da Universidade de Aveiro (RIA) |
op_collection_id |
ftria |
language |
English |
topic |
Inorganic mercury Marine macroalgae Fish Bioaccumulation Antioxidant and geno-protection Functional foods |
spellingShingle |
Inorganic mercury Marine macroalgae Fish Bioaccumulation Antioxidant and geno-protection Functional foods Neto, Ana Catarina Canastra Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish |
topic_facet |
Inorganic mercury Marine macroalgae Fish Bioaccumulation Antioxidant and geno-protection Functional foods |
description |
Marine macroalgae (MM) present a great biological potential since they are a source of many essential natural compounds with physiological benefits. Despite this, there is little knowledge about the impact of MM supplementation on protecting fish health from exposure to environmental contaminants. Mercury (Hg) is well known for its high toxicity, and it can be found at low levels in farm-raised fish. The present dissertation was design in this context, with the aim of addressing a possible protection by a diet supplemented with MM to the juvenile white sea bream (Diplodus sargus), particularly after exposure via water to realistic levels of inorganic Hg (iHg). Thus, the toxicokinetics of iHg was studied, antioxidant protection and neuroprotection as well as anti-genotoxic properties. For this purpose, the fish were fed for 3 months with a MM-enriched diet [total incorporation of 5 %, with the species Fucus vesiculosus, Gracilaria gracilis and Ulva rigida in equal parts - algae-supplemented fish group (AC)], while the non-supplemented fish were fed with a standard feed (SC). Then, both groups on different baseline diets were exposed to realistic levels of iHg (2 μg L-1) via water for 7 days (E7) (AHg and SHg groups), followed by a 14-day post-exposure period (PE14). Control groups, not exposed to iHg, were maintained throughout the experiment (AC and SC). At E7 and PE14, the fish from the different groups (AC, SC, AHg, SHg) were sacrificed and the gills, blood, liver and brain were collected to determine total Hg (tHg) levels, in parallel with the assessment of oxidative stress related endpoints in the gills, liver and brain, where the following biochemical parameters were determined: (i) enzymatic antioxidants [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GR)] and non-enzymatic antioxidants (total glutathione (GSHt)); (ii) damage indicators (lipid peroxidation - LPO); (iii) acetylcholinesterase (AChE) activity, used as an ... |
author2 |
Kowalski, Patrícia Pereira Cesário, Rute Isabel Telhadas |
format |
Master Thesis |
author |
Neto, Ana Catarina Canastra |
author_facet |
Neto, Ana Catarina Canastra |
author_sort |
Neto, Ana Catarina Canastra |
title |
Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish |
title_short |
Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish |
title_full |
Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish |
title_fullStr |
Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish |
title_full_unstemmed |
Protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish |
title_sort |
protection of a marine macroalgae-enriched diet against mercury accumulation and related biochemical and genotoxic effects in fish |
publishDate |
1483 |
url |
http://hdl.handle.net/10773/41133 |
genre |
White Sea |
genre_facet |
White Sea |
op_relation |
http://hdl.handle.net/10773/41133 |
op_rights |
embargoedAccess https://creativecommons.org/licenses/by/4.0/ |
_version_ |
1796933401931939840 |