Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure
Adult eels (Anguilla anguilla L.) were exposed for 8, 16, 24, and 72 h to 0, 0.1, 0.3, 0.9, and 2.7 microM abietic acid (AA). Genotoxicity was measured as erythrocytic nuclear abnormalities (ENA), as well as DNA strand breaks in blood and liver. Liver cytochrome P450 (P450) content, liver ethoxyreso...
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ftria:oai:ria.ua.pt:10773/27583 2023-05-15T13:27:20+02:00 Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure Maria, V. L. Correia, A. C. Santos, M. A. 2004-06 http://hdl.handle.net/10773/27583 https://doi.org/10.1016/j.ecoenv.2003.12.005 eng eng Elsevier PCNA/C/BIA/175-96 BD/18254/98 0147-6513 http://hdl.handle.net/10773/27583 doi:10.1016/j.ecoenv.2003.12.005 1090-2414 openAccess Genotoxicity DNA strand breaks Liver ethoxyresorufin-O-deethylase Erthrocytic nuclear abnormalities Eel article 2004 ftria https://doi.org/10.1016/j.ecoenv.2003.12.005 2022-05-25T18:38:01Z Adult eels (Anguilla anguilla L.) were exposed for 8, 16, 24, and 72 h to 0, 0.1, 0.3, 0.9, and 2.7 microM abietic acid (AA). Genotoxicity was measured as erythrocytic nuclear abnormalities (ENA), as well as DNA strand breaks in blood and liver. Liver cytochrome P450 (P450) content, liver ethoxyresorufin O-deethylase (EROD), and glutathione S-transferase (GST) activities were determined as biotransformation biomarkers. Liver alanine transaminase (ALT) activity was also measured as an indication of tissue damage. Low AA concentrations, such as 0.1 and 0.3 microM, result in a delayed induction of A. anguilla L. liver EROD activity, whereas the higher AA concentration (2.7 microM AA) also has a delayed effect probably as a consequence of liver tissue high inhibitory concentration. The current eel liver GST activity results demonstrate that only low AA concentrations promote liver increases in GST, whereas high AA concentrations, such as 0.9 and 2.7 microM, do not alter it. The results concerning eel liver ALT activity indicate that significant liver damage is induced by high AA concentrations, such as 2.7 and 0.9 microM. The eel ENA result analysis reveals that AA is a weak ENA inducer in A. anguilla L. Blood DNA integrity results suggest that low AA concentrations promote late decreases in blood DNA integrity; nevertheless, high AA concentrations are early blood genotoxic inducers compared with low AA doses. According to the present research results with respect to eel liver DNA damage, all of the AA exposure concentrations decreased liver DNA integrity. published Article in Journal/Newspaper Anguilla anguilla Repositório Institucional da Universidade de Aveiro (RIA) Ecotoxicology and Environmental Safety 58 2 202 210 |
institution |
Open Polar |
collection |
Repositório Institucional da Universidade de Aveiro (RIA) |
op_collection_id |
ftria |
language |
English |
topic |
Genotoxicity DNA strand breaks Liver ethoxyresorufin-O-deethylase Erthrocytic nuclear abnormalities Eel |
spellingShingle |
Genotoxicity DNA strand breaks Liver ethoxyresorufin-O-deethylase Erthrocytic nuclear abnormalities Eel Maria, V. L. Correia, A. C. Santos, M. A. Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure |
topic_facet |
Genotoxicity DNA strand breaks Liver ethoxyresorufin-O-deethylase Erthrocytic nuclear abnormalities Eel |
description |
Adult eels (Anguilla anguilla L.) were exposed for 8, 16, 24, and 72 h to 0, 0.1, 0.3, 0.9, and 2.7 microM abietic acid (AA). Genotoxicity was measured as erythrocytic nuclear abnormalities (ENA), as well as DNA strand breaks in blood and liver. Liver cytochrome P450 (P450) content, liver ethoxyresorufin O-deethylase (EROD), and glutathione S-transferase (GST) activities were determined as biotransformation biomarkers. Liver alanine transaminase (ALT) activity was also measured as an indication of tissue damage. Low AA concentrations, such as 0.1 and 0.3 microM, result in a delayed induction of A. anguilla L. liver EROD activity, whereas the higher AA concentration (2.7 microM AA) also has a delayed effect probably as a consequence of liver tissue high inhibitory concentration. The current eel liver GST activity results demonstrate that only low AA concentrations promote liver increases in GST, whereas high AA concentrations, such as 0.9 and 2.7 microM, do not alter it. The results concerning eel liver ALT activity indicate that significant liver damage is induced by high AA concentrations, such as 2.7 and 0.9 microM. The eel ENA result analysis reveals that AA is a weak ENA inducer in A. anguilla L. Blood DNA integrity results suggest that low AA concentrations promote late decreases in blood DNA integrity; nevertheless, high AA concentrations are early blood genotoxic inducers compared with low AA doses. According to the present research results with respect to eel liver DNA damage, all of the AA exposure concentrations decreased liver DNA integrity. published |
format |
Article in Journal/Newspaper |
author |
Maria, V. L. Correia, A. C. Santos, M. A. |
author_facet |
Maria, V. L. Correia, A. C. Santos, M. A. |
author_sort |
Maria, V. L. |
title |
Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure |
title_short |
Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure |
title_full |
Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure |
title_fullStr |
Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure |
title_full_unstemmed |
Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure |
title_sort |
anguilla anguilla l. genotoxic and liver biotransformation responses to abietic acid exposure |
publisher |
Elsevier |
publishDate |
2004 |
url |
http://hdl.handle.net/10773/27583 https://doi.org/10.1016/j.ecoenv.2003.12.005 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
PCNA/C/BIA/175-96 BD/18254/98 0147-6513 http://hdl.handle.net/10773/27583 doi:10.1016/j.ecoenv.2003.12.005 1090-2414 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.ecoenv.2003.12.005 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
58 |
container_issue |
2 |
container_start_page |
202 |
op_container_end_page |
210 |
_version_ |
1766397769765879808 |