Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure

Adult eels (Anguilla anguilla L.) were exposed for 8, 16, 24, and 72 h to 0, 0.1, 0.3, 0.9, and 2.7 microM abietic acid (AA). Genotoxicity was measured as erythrocytic nuclear abnormalities (ENA), as well as DNA strand breaks in blood and liver. Liver cytochrome P450 (P450) content, liver ethoxyreso...

Full description

Bibliographic Details
Published in:Ecotoxicology and Environmental Safety
Main Authors: Maria, V. L., Correia, A. C., Santos, M. A.
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2004
Subjects:
Eel
Online Access:http://hdl.handle.net/10773/27583
https://doi.org/10.1016/j.ecoenv.2003.12.005
id ftria:oai:ria.ua.pt:10773/27583
record_format openpolar
spelling ftria:oai:ria.ua.pt:10773/27583 2023-05-15T13:27:20+02:00 Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure Maria, V. L. Correia, A. C. Santos, M. A. 2004-06 http://hdl.handle.net/10773/27583 https://doi.org/10.1016/j.ecoenv.2003.12.005 eng eng Elsevier PCNA/C/BIA/175-96 BD/18254/98 0147-6513 http://hdl.handle.net/10773/27583 doi:10.1016/j.ecoenv.2003.12.005 1090-2414 openAccess Genotoxicity DNA strand breaks Liver ethoxyresorufin-O-deethylase Erthrocytic nuclear abnormalities Eel article 2004 ftria https://doi.org/10.1016/j.ecoenv.2003.12.005 2022-05-25T18:38:01Z Adult eels (Anguilla anguilla L.) were exposed for 8, 16, 24, and 72 h to 0, 0.1, 0.3, 0.9, and 2.7 microM abietic acid (AA). Genotoxicity was measured as erythrocytic nuclear abnormalities (ENA), as well as DNA strand breaks in blood and liver. Liver cytochrome P450 (P450) content, liver ethoxyresorufin O-deethylase (EROD), and glutathione S-transferase (GST) activities were determined as biotransformation biomarkers. Liver alanine transaminase (ALT) activity was also measured as an indication of tissue damage. Low AA concentrations, such as 0.1 and 0.3 microM, result in a delayed induction of A. anguilla L. liver EROD activity, whereas the higher AA concentration (2.7 microM AA) also has a delayed effect probably as a consequence of liver tissue high inhibitory concentration. The current eel liver GST activity results demonstrate that only low AA concentrations promote liver increases in GST, whereas high AA concentrations, such as 0.9 and 2.7 microM, do not alter it. The results concerning eel liver ALT activity indicate that significant liver damage is induced by high AA concentrations, such as 2.7 and 0.9 microM. The eel ENA result analysis reveals that AA is a weak ENA inducer in A. anguilla L. Blood DNA integrity results suggest that low AA concentrations promote late decreases in blood DNA integrity; nevertheless, high AA concentrations are early blood genotoxic inducers compared with low AA doses. According to the present research results with respect to eel liver DNA damage, all of the AA exposure concentrations decreased liver DNA integrity. published Article in Journal/Newspaper Anguilla anguilla Repositório Institucional da Universidade de Aveiro (RIA) Ecotoxicology and Environmental Safety 58 2 202 210
institution Open Polar
collection Repositório Institucional da Universidade de Aveiro (RIA)
op_collection_id ftria
language English
topic Genotoxicity
DNA strand breaks
Liver ethoxyresorufin-O-deethylase
Erthrocytic nuclear abnormalities
Eel
spellingShingle Genotoxicity
DNA strand breaks
Liver ethoxyresorufin-O-deethylase
Erthrocytic nuclear abnormalities
Eel
Maria, V. L.
Correia, A. C.
Santos, M. A.
Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure
topic_facet Genotoxicity
DNA strand breaks
Liver ethoxyresorufin-O-deethylase
Erthrocytic nuclear abnormalities
Eel
description Adult eels (Anguilla anguilla L.) were exposed for 8, 16, 24, and 72 h to 0, 0.1, 0.3, 0.9, and 2.7 microM abietic acid (AA). Genotoxicity was measured as erythrocytic nuclear abnormalities (ENA), as well as DNA strand breaks in blood and liver. Liver cytochrome P450 (P450) content, liver ethoxyresorufin O-deethylase (EROD), and glutathione S-transferase (GST) activities were determined as biotransformation biomarkers. Liver alanine transaminase (ALT) activity was also measured as an indication of tissue damage. Low AA concentrations, such as 0.1 and 0.3 microM, result in a delayed induction of A. anguilla L. liver EROD activity, whereas the higher AA concentration (2.7 microM AA) also has a delayed effect probably as a consequence of liver tissue high inhibitory concentration. The current eel liver GST activity results demonstrate that only low AA concentrations promote liver increases in GST, whereas high AA concentrations, such as 0.9 and 2.7 microM, do not alter it. The results concerning eel liver ALT activity indicate that significant liver damage is induced by high AA concentrations, such as 2.7 and 0.9 microM. The eel ENA result analysis reveals that AA is a weak ENA inducer in A. anguilla L. Blood DNA integrity results suggest that low AA concentrations promote late decreases in blood DNA integrity; nevertheless, high AA concentrations are early blood genotoxic inducers compared with low AA doses. According to the present research results with respect to eel liver DNA damage, all of the AA exposure concentrations decreased liver DNA integrity. published
format Article in Journal/Newspaper
author Maria, V. L.
Correia, A. C.
Santos, M. A.
author_facet Maria, V. L.
Correia, A. C.
Santos, M. A.
author_sort Maria, V. L.
title Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure
title_short Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure
title_full Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure
title_fullStr Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure
title_full_unstemmed Anguilla anguilla L. genotoxic and liver biotransformation responses to abietic acid exposure
title_sort anguilla anguilla l. genotoxic and liver biotransformation responses to abietic acid exposure
publisher Elsevier
publishDate 2004
url http://hdl.handle.net/10773/27583
https://doi.org/10.1016/j.ecoenv.2003.12.005
genre Anguilla anguilla
genre_facet Anguilla anguilla
op_relation PCNA/C/BIA/175-96
BD/18254/98
0147-6513
http://hdl.handle.net/10773/27583
doi:10.1016/j.ecoenv.2003.12.005
1090-2414
op_rights openAccess
op_doi https://doi.org/10.1016/j.ecoenv.2003.12.005
container_title Ecotoxicology and Environmental Safety
container_volume 58
container_issue 2
container_start_page 202
op_container_end_page 210
_version_ 1766397769765879808