Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum
Several works evaluated the toxicity of pharmaceutical drugs and climate related changes in invertebrates but few explored the combined effects of both stressors, namely considering their mode of action (MoA). Carbamazepine (CBZ) and cetirizine (CTZ) are pharmaceutical drugs detected in the environm...
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Online Access: | http://hdl.handle.net/10773/27384 https://doi.org/10.1016/j.envpol.2017.12.121 |
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ftria:oai:ria.ua.pt:10773/27384 2023-05-15T17:50:51+02:00 Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum Almeida, Ângela Freitas, Rosa Calisto, Vânia Esteves, Valdemar I. Schneider, Rudolf J. Soares, Amadeu M. V. M. Figueira, Etelvina Campos, Bruno Barata, Carlos 2018-04 http://hdl.handle.net/10773/27384 https://doi.org/10.1016/j.envpol.2017.12.121 eng eng Elsevier info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78645%2F2011/PT SFRH/BD/110218/2015 info:eu-repo/grantAgreement/FCT/5876/147273/PT 0269-7491 http://hdl.handle.net/10773/27384 https://doi.org/10.1016/j.envpol.2017.12.121 1873-6424 restrictedAccess Pharmaceuticals Mode of action Biomarkers Transcriptomic Bivalves article 2018 ftria https://doi.org/10.1016/j.envpol.2017.12.121 2022-05-25T18:37:57Z Several works evaluated the toxicity of pharmaceutical drugs and climate related changes in invertebrates but few explored the combined effects of both stressors, namely considering their mode of action (MoA). Carbamazepine (CBZ) and cetirizine (CTZ) are pharmaceutical drugs detected in the environment and the toxicity derived from the combined effects of these drugs with ocean acidification (OA) is poorly explored. Thus, the present study investigated the biochemical parameters related to an oxidative stress response and the transcription of genes related to the MoA of CBZ (1.0 μg/L) and CTZ (0.6 μg/L) in the clam Ruditapes philippinarum chronically exposed (28 days) to control (7.8) and low (7.5) pH conditions. The results obtained showed that despite the clams accumulated both drugs, at low pH the clams exposed to CTZ decreased drug concentration and BCF values (CTZ uptake: 2.0 ± 0.5 ng/g fresh weight; BCF: 3.8 ± 0.9) in comparison with clams exposed to control pH (CTZ uptake: 2.9 ± 0.3 ng/g fresh weight; BCF: 5.5 ± 0.6). No oxidative stress was induced by the exposure to CBZ or CTZ at each pH level, but the transcription of several genes related with the MoA (neurotransmission, immunity and biomineralization) was altered by low pH, drug exposure and the combination of both stressors. At both pH conditions, CBZ increased the transcription of GABA receptor gene (neurotransmission) and CTZ led to a decrease of Perlucin gene (biomineralization) transcription. The transcription of MyD88 gene (immunity) decreased at low pH (7.5) combined with drug exposure (CBZ or CTZ). Thus, it was highlighted that the interaction of drug exposure and low pH conditions can change bivalves’ sensitivity to drugs or alter drugs toxicity. published Article in Journal/Newspaper Ocean acidification Repositório Institucional da Universidade de Aveiro (RIA) Environmental Pollution 235 857 868 |
institution |
Open Polar |
collection |
Repositório Institucional da Universidade de Aveiro (RIA) |
op_collection_id |
ftria |
language |
English |
topic |
Pharmaceuticals Mode of action Biomarkers Transcriptomic Bivalves |
spellingShingle |
Pharmaceuticals Mode of action Biomarkers Transcriptomic Bivalves Almeida, Ângela Freitas, Rosa Calisto, Vânia Esteves, Valdemar I. Schneider, Rudolf J. Soares, Amadeu M. V. M. Figueira, Etelvina Campos, Bruno Barata, Carlos Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum |
topic_facet |
Pharmaceuticals Mode of action Biomarkers Transcriptomic Bivalves |
description |
Several works evaluated the toxicity of pharmaceutical drugs and climate related changes in invertebrates but few explored the combined effects of both stressors, namely considering their mode of action (MoA). Carbamazepine (CBZ) and cetirizine (CTZ) are pharmaceutical drugs detected in the environment and the toxicity derived from the combined effects of these drugs with ocean acidification (OA) is poorly explored. Thus, the present study investigated the biochemical parameters related to an oxidative stress response and the transcription of genes related to the MoA of CBZ (1.0 μg/L) and CTZ (0.6 μg/L) in the clam Ruditapes philippinarum chronically exposed (28 days) to control (7.8) and low (7.5) pH conditions. The results obtained showed that despite the clams accumulated both drugs, at low pH the clams exposed to CTZ decreased drug concentration and BCF values (CTZ uptake: 2.0 ± 0.5 ng/g fresh weight; BCF: 3.8 ± 0.9) in comparison with clams exposed to control pH (CTZ uptake: 2.9 ± 0.3 ng/g fresh weight; BCF: 5.5 ± 0.6). No oxidative stress was induced by the exposure to CBZ or CTZ at each pH level, but the transcription of several genes related with the MoA (neurotransmission, immunity and biomineralization) was altered by low pH, drug exposure and the combination of both stressors. At both pH conditions, CBZ increased the transcription of GABA receptor gene (neurotransmission) and CTZ led to a decrease of Perlucin gene (biomineralization) transcription. The transcription of MyD88 gene (immunity) decreased at low pH (7.5) combined with drug exposure (CBZ or CTZ). Thus, it was highlighted that the interaction of drug exposure and low pH conditions can change bivalves’ sensitivity to drugs or alter drugs toxicity. published |
format |
Article in Journal/Newspaper |
author |
Almeida, Ângela Freitas, Rosa Calisto, Vânia Esteves, Valdemar I. Schneider, Rudolf J. Soares, Amadeu M. V. M. Figueira, Etelvina Campos, Bruno Barata, Carlos |
author_facet |
Almeida, Ângela Freitas, Rosa Calisto, Vânia Esteves, Valdemar I. Schneider, Rudolf J. Soares, Amadeu M. V. M. Figueira, Etelvina Campos, Bruno Barata, Carlos |
author_sort |
Almeida, Ângela |
title |
Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum |
title_short |
Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum |
title_full |
Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum |
title_fullStr |
Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum |
title_full_unstemmed |
Effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam Ruditapes philippinarum |
title_sort |
effects of carbamazepine and cetirizine under an ocean acidification scenario on the biochemical and transcriptome responses of the clam ruditapes philippinarum |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://hdl.handle.net/10773/27384 https://doi.org/10.1016/j.envpol.2017.12.121 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78645%2F2011/PT SFRH/BD/110218/2015 info:eu-repo/grantAgreement/FCT/5876/147273/PT 0269-7491 http://hdl.handle.net/10773/27384 https://doi.org/10.1016/j.envpol.2017.12.121 1873-6424 |
op_rights |
restrictedAccess |
op_doi |
https://doi.org/10.1016/j.envpol.2017.12.121 |
container_title |
Environmental Pollution |
container_volume |
235 |
container_start_page |
857 |
op_container_end_page |
868 |
_version_ |
1766157764377182208 |