Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost
Dietary long chain polyunsaturated fatty acids (FA) have been recognized of crucial importance in early development of vertebrates, contributing to the impressive morphological and physiological changes both as building blocks and to energy production. The importance of lipids along development depe...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Online Access: | http://hdl.handle.net/10773/25617 https://doi.org/10.1016/j.cbpb.2013.01.003 |
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ftria:oai:ria.ua.pt:10773/25617 2023-05-15T18:15:50+02:00 Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost Cunha, I. Galante-Oliveira, S. Rocha, E. Planas, M. Urbatzka, R. Castro, L. F. C. 2013 http://hdl.handle.net/10773/25617 https://doi.org/10.1016/j.cbpb.2013.01.003 eng eng Elsevier info:eu-repo/grantAgreement/FCT/5876-PPCDTI/68885/PT 1096-4959 http://hdl.handle.net/10773/25617 doi:10.1016/j.cbpb.2013.01.003 restrictedAccess https://creativecommons.org/licenses/by/4.0/ CC-BY Fish embryos Gene transcription Lipid metabolism Peroxisome proliferator activated receptors Scophthalmus maximus article 2013 ftria https://doi.org/10.1016/j.cbpb.2013.01.003 2022-05-25T18:37:02Z Dietary long chain polyunsaturated fatty acids (FA) have been recognized of crucial importance in early development of vertebrates, contributing to the impressive morphological and physiological changes both as building blocks and to energy production. The importance of lipids along development depends on ontogenetic, phylogenetic and environmental parameters. The expression patterns of FA metabolism genes have not been characterized in developing fish embryos nor compared to lipid class profiles. Full lipid metabolism only occurred after hatching, as revealed by alterations in lipid profiles and FA gene expression. Nonetheless, transcriptional changes of some FA genes were already present in embryos at notochord formation. Many genes displayed an expression profile opposed to the decrease of lipids along the development, while others responded solely to starvation. Transcription of most genes involved in FA metabolism had a strong correlation to PPARs' mRNA levels (α1, α2, β, γ). The comparison of mRNA expression of the genes with the lipid profiles produced new insights into the FA metabolism and regulation during the development of turbot larvae, providing the basis for future studies including comparative approaches with other vertebrate species. We would like thank Insuiña–Pescanova S.A., for kindly supplying the embryos used in the experiments described in this paper. published Article in Journal/Newspaper Scophthalmus maximus Turbot Repositório Institucional da Universidade de Aveiro (RIA) Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 164 4 247 258 |
institution |
Open Polar |
collection |
Repositório Institucional da Universidade de Aveiro (RIA) |
op_collection_id |
ftria |
language |
English |
topic |
Fish embryos Gene transcription Lipid metabolism Peroxisome proliferator activated receptors Scophthalmus maximus |
spellingShingle |
Fish embryos Gene transcription Lipid metabolism Peroxisome proliferator activated receptors Scophthalmus maximus Cunha, I. Galante-Oliveira, S. Rocha, E. Planas, M. Urbatzka, R. Castro, L. F. C. Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost |
topic_facet |
Fish embryos Gene transcription Lipid metabolism Peroxisome proliferator activated receptors Scophthalmus maximus |
description |
Dietary long chain polyunsaturated fatty acids (FA) have been recognized of crucial importance in early development of vertebrates, contributing to the impressive morphological and physiological changes both as building blocks and to energy production. The importance of lipids along development depends on ontogenetic, phylogenetic and environmental parameters. The expression patterns of FA metabolism genes have not been characterized in developing fish embryos nor compared to lipid class profiles. Full lipid metabolism only occurred after hatching, as revealed by alterations in lipid profiles and FA gene expression. Nonetheless, transcriptional changes of some FA genes were already present in embryos at notochord formation. Many genes displayed an expression profile opposed to the decrease of lipids along the development, while others responded solely to starvation. Transcription of most genes involved in FA metabolism had a strong correlation to PPARs' mRNA levels (α1, α2, β, γ). The comparison of mRNA expression of the genes with the lipid profiles produced new insights into the FA metabolism and regulation during the development of turbot larvae, providing the basis for future studies including comparative approaches with other vertebrate species. We would like thank Insuiña–Pescanova S.A., for kindly supplying the embryos used in the experiments described in this paper. published |
format |
Article in Journal/Newspaper |
author |
Cunha, I. Galante-Oliveira, S. Rocha, E. Planas, M. Urbatzka, R. Castro, L. F. C. |
author_facet |
Cunha, I. Galante-Oliveira, S. Rocha, E. Planas, M. Urbatzka, R. Castro, L. F. C. |
author_sort |
Cunha, I. |
title |
Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost |
title_short |
Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost |
title_full |
Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost |
title_fullStr |
Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost |
title_full_unstemmed |
Dynamics of PPARs, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost |
title_sort |
dynamics of ppars, fatty acid metabolism genes and lipid classes in eggs and early larvae of a teleost |
publisher |
Elsevier |
publishDate |
2013 |
url |
http://hdl.handle.net/10773/25617 https://doi.org/10.1016/j.cbpb.2013.01.003 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/68885/PT 1096-4959 http://hdl.handle.net/10773/25617 doi:10.1016/j.cbpb.2013.01.003 |
op_rights |
restrictedAccess https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.cbpb.2013.01.003 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
164 |
container_issue |
4 |
container_start_page |
247 |
op_container_end_page |
258 |
_version_ |
1766189058242904064 |