Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643
Peroxisome proliferator-activated receptors (PPARs) are involved in the regulation of lipid and carbohydrate metabolism and can be activated either by natural ligands as fatty acids or by synthetic ligands including several environmental chemicals. In this study, two PPARα isoforms (α1 and α2) were...
Published in: | Fish Physiology and Biochemistry |
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2013
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Online Access: | http://hdl.handle.net/10773/24600 https://doi.org/10.1007/s10695-012-9761-7 |
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ftria:oai:ria.ua.pt:10773/24600 2023-05-15T18:15:42+02:00 Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643 Urbatzka, R. Galante-Oliveira, S. Rocha, E. Castro, L. F. C. Cunha, I. 2013 http://hdl.handle.net/10773/24600 https://doi.org/10.1007/s10695-012-9761-7 eng eng Springer Verlag info:eu-repo/grantAgreement/FCT/5876-PPCDTI/68885/PT 0920-1742 http://hdl.handle.net/10773/24600 doi:10.1007/s10695-012-9761-7 1573-5168 restrictedAccess https://creativecommons.org/licenses/by/4.0/ CC-BY Myosin light chain 2 PPARα Peroxisome proliferators Scophthalmus maximus Tropomyosin 4-1 WY-14643 article 2013 ftria https://doi.org/10.1007/s10695-012-9761-7 2022-05-25T18:37:05Z Peroxisome proliferator-activated receptors (PPARs) are involved in the regulation of lipid and carbohydrate metabolism and can be activated either by natural ligands as fatty acids or by synthetic ligands including several environmental chemicals. In this study, two PPARα isoforms (α1 and α2) were analyzed in turbot (Scophthalmus maximus) for a different tissue distribution. PPARα1 was ubiquitously expressed, while the PPARα2 was predominantly expressed in the heart. Following this result, turbot juveniles were exposed by injection to a synthetic selective PPARα agonist, WY-14643, for 14 days. Suppression subtractive hybridization (SSH) was performed with pools of heart samples of control and exposed fish to get insights into PPARα-regulated genes in the heart of juvenile turbot. Four genes were positively identified in the forward-subtracted and 12 genes in the reverse-subtracted cDNA SSH library, corresponding to the down-regulated and up-regulated genes in response to the WY-14643 treatment, respectively. The confirmation of these results in individual samples of juvenile turbot exposed to WY-14643 revealed a statistically significant mRNA induction of two cardiac muscle proteins (myosin light chain 2 and tropomyosin 4), which were shown to be involved in heart contraction and heartbeat regulation in other teleost species. Herewith, we showed for the first time that PPARα2 is predominantly expressed in the heart and that a PPARα agonist can induce the mRNA expression of cardiac muscle proteins in teleosts. This work was financially supported via the project PTDC/MAR/68885/2006, funded by the Portuguese Foundation for Science and Technology (FCT) and by the ‘‘Programa Operacional Ciência e Inovação 2010’’ (POCI 2010), co-financed by the FEDER European Community fund. We would like to thank Professor António Afonso for having kindly provided the experimental fish. published Article in Journal/Newspaper Scophthalmus maximus Turbot Repositório Institucional da Universidade de Aveiro (RIA) Fish Physiology and Biochemistry 39 4 1043 1055 |
institution |
Open Polar |
collection |
Repositório Institucional da Universidade de Aveiro (RIA) |
op_collection_id |
ftria |
language |
English |
topic |
Myosin light chain 2 PPARα Peroxisome proliferators Scophthalmus maximus Tropomyosin 4-1 WY-14643 |
spellingShingle |
Myosin light chain 2 PPARα Peroxisome proliferators Scophthalmus maximus Tropomyosin 4-1 WY-14643 Urbatzka, R. Galante-Oliveira, S. Rocha, E. Castro, L. F. C. Cunha, I. Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643 |
topic_facet |
Myosin light chain 2 PPARα Peroxisome proliferators Scophthalmus maximus Tropomyosin 4-1 WY-14643 |
description |
Peroxisome proliferator-activated receptors (PPARs) are involved in the regulation of lipid and carbohydrate metabolism and can be activated either by natural ligands as fatty acids or by synthetic ligands including several environmental chemicals. In this study, two PPARα isoforms (α1 and α2) were analyzed in turbot (Scophthalmus maximus) for a different tissue distribution. PPARα1 was ubiquitously expressed, while the PPARα2 was predominantly expressed in the heart. Following this result, turbot juveniles were exposed by injection to a synthetic selective PPARα agonist, WY-14643, for 14 days. Suppression subtractive hybridization (SSH) was performed with pools of heart samples of control and exposed fish to get insights into PPARα-regulated genes in the heart of juvenile turbot. Four genes were positively identified in the forward-subtracted and 12 genes in the reverse-subtracted cDNA SSH library, corresponding to the down-regulated and up-regulated genes in response to the WY-14643 treatment, respectively. The confirmation of these results in individual samples of juvenile turbot exposed to WY-14643 revealed a statistically significant mRNA induction of two cardiac muscle proteins (myosin light chain 2 and tropomyosin 4), which were shown to be involved in heart contraction and heartbeat regulation in other teleost species. Herewith, we showed for the first time that PPARα2 is predominantly expressed in the heart and that a PPARα agonist can induce the mRNA expression of cardiac muscle proteins in teleosts. This work was financially supported via the project PTDC/MAR/68885/2006, funded by the Portuguese Foundation for Science and Technology (FCT) and by the ‘‘Programa Operacional Ciência e Inovação 2010’’ (POCI 2010), co-financed by the FEDER European Community fund. We would like to thank Professor António Afonso for having kindly provided the experimental fish. published |
format |
Article in Journal/Newspaper |
author |
Urbatzka, R. Galante-Oliveira, S. Rocha, E. Castro, L. F. C. Cunha, I. |
author_facet |
Urbatzka, R. Galante-Oliveira, S. Rocha, E. Castro, L. F. C. Cunha, I. |
author_sort |
Urbatzka, R. |
title |
Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643 |
title_short |
Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643 |
title_full |
Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643 |
title_fullStr |
Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643 |
title_full_unstemmed |
Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643 |
title_sort |
tissue expression of ppar-alpha isoforms in scophthalmus maximus and transcriptional response of target genes in the heart after exposure to wy-14643 |
publisher |
Springer Verlag |
publishDate |
2013 |
url |
http://hdl.handle.net/10773/24600 https://doi.org/10.1007/s10695-012-9761-7 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/68885/PT 0920-1742 http://hdl.handle.net/10773/24600 doi:10.1007/s10695-012-9761-7 1573-5168 |
op_rights |
restrictedAccess https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s10695-012-9761-7 |
container_title |
Fish Physiology and Biochemistry |
container_volume |
39 |
container_issue |
4 |
container_start_page |
1043 |
op_container_end_page |
1055 |
_version_ |
1766188890167705600 |