Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.)
This study aimed to assess the mechanisms of innate immune function responses to silica-coated iron oxide nanoparticle functionalized with dithiocarbamate groups (IONP) exposure alone and its associated mercury (Hg) in European eel (Anguilla anguilla L.) phagocytes isolated from peritoneum (P-phagoc...
Published in: | Environmental Science and Pollution Research |
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Language: | English |
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SPRINGER HEIDELBERG
2015
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Online Access: | http://hdl.handle.net/10773/21007 https://doi.org/10.1007/s11356-015-5375-9 |
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ftria:oai:ria.ua.pt:10773/21007 2023-05-15T13:27:30+02:00 Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.) Costa, Leonor C. Mohmood, Iram Trindade, Tito Saleem, Mohammad Duarte, Armando C. Pereira, Eduarda Ahmad, Iqbal 2015 http://hdl.handle.net/10773/21007 https://doi.org/10.1007/s11356-015-5375-9 eng eng SPRINGER HEIDELBERG info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F74410%2F2010/PT info:eu-repo/grantAgreement/FCT/COMPETE/132951/PT info:eu-repo/grantAgreement/FCT/5876-PPCDTI/129903/PT info:eu-repo/grantAgreement/FCT/5876/147332/PT 10.1007/s11356-015-5375-9 0944-1344 http://hdl.handle.net/10773/21007 doi:10.1007/s11356-015-5375-9 restrictedAccess IRON-OXIDE NANOPARTICLES MILL EFFLUENT EXPOSURE MR CONTRAST AGENTS IN-VITRO MANUFACTURED NANOPARTICLES ENGINEERED NANOPARTICLES PEROXIDATIVE DAMAGE PHAGOCYTIC-ACTIVITY FISH PARTICLES article 2015 ftria https://doi.org/10.1007/s11356-015-5375-9 2022-05-25T18:36:26Z This study aimed to assess the mechanisms of innate immune function responses to silica-coated iron oxide nanoparticle functionalized with dithiocarbamate groups (IONP) exposure alone and its associated mercury (Hg) in European eel (Anguilla anguilla L.) phagocytes isolated from peritoneum (P-phagocytes), gill (G-phagocytes), head kidney (HK-phagocytes) and spleen (S-phagocytes). The study evaluated viability, phagocytosis, oxidative burst activity (OBA) and lipid peroxidation (LPO). Four groups were made: (1) 2 x 10(6) phagocytes + RPMI-1640 (control), (2) 2 x 10(6) phagocytes + IONP (2.5 mg L-1), (3) 2 x 10(6) phagocytes + Hg (50 mu g L-1) and (4) 2 x 10(6) phagocytes + IONP + Hg. Samplings were performed at 0, 2, 4, 8, 16, 24, 48 and 72 h of exposure. A. anguilla P-, G-, HK- and S-phagocytes in vitro exposure to IONP alone revealed either increased (except HK-phagocytes at 16 h) or no change in viability, suggesting that the cells are metabolically active and resistant to IONP exposure alone. In terms of phagocytes overactivation and reactive oxygen species (ROS) production as an indirect mechanism of immunotoxicity, the phagocytes responded in the following manner: P->S->HK-=G-phagocytes for IONP exposure alone, S->HK->P-=G-phagocytes for Hg exposure alone and HK->G-=S->P-phagocytes for concomitant exposure. Overall, considering Hg as a surrogate for metals and its association with IONP, as well as the likelihood that it could pose a serious threat to aquatic organisms by modulating their immune defense mechanisms if accidentally discharged into the aquatic environment, current results suggest that the step of IONP-metal complex removal must not be underrated and should be processed without any more ado. Article in Journal/Newspaper Anguilla anguilla European eel Repositório Institucional da Universidade de Aveiro (RIA) Environmental Science and Pollution Research 22 23 18574 18589 |
institution |
Open Polar |
collection |
Repositório Institucional da Universidade de Aveiro (RIA) |
op_collection_id |
ftria |
language |
English |
topic |
IRON-OXIDE NANOPARTICLES MILL EFFLUENT EXPOSURE MR CONTRAST AGENTS IN-VITRO MANUFACTURED NANOPARTICLES ENGINEERED NANOPARTICLES PEROXIDATIVE DAMAGE PHAGOCYTIC-ACTIVITY FISH PARTICLES |
spellingShingle |
IRON-OXIDE NANOPARTICLES MILL EFFLUENT EXPOSURE MR CONTRAST AGENTS IN-VITRO MANUFACTURED NANOPARTICLES ENGINEERED NANOPARTICLES PEROXIDATIVE DAMAGE PHAGOCYTIC-ACTIVITY FISH PARTICLES Costa, Leonor C. Mohmood, Iram Trindade, Tito Saleem, Mohammad Duarte, Armando C. Pereira, Eduarda Ahmad, Iqbal Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.) |
topic_facet |
IRON-OXIDE NANOPARTICLES MILL EFFLUENT EXPOSURE MR CONTRAST AGENTS IN-VITRO MANUFACTURED NANOPARTICLES ENGINEERED NANOPARTICLES PEROXIDATIVE DAMAGE PHAGOCYTIC-ACTIVITY FISH PARTICLES |
description |
This study aimed to assess the mechanisms of innate immune function responses to silica-coated iron oxide nanoparticle functionalized with dithiocarbamate groups (IONP) exposure alone and its associated mercury (Hg) in European eel (Anguilla anguilla L.) phagocytes isolated from peritoneum (P-phagocytes), gill (G-phagocytes), head kidney (HK-phagocytes) and spleen (S-phagocytes). The study evaluated viability, phagocytosis, oxidative burst activity (OBA) and lipid peroxidation (LPO). Four groups were made: (1) 2 x 10(6) phagocytes + RPMI-1640 (control), (2) 2 x 10(6) phagocytes + IONP (2.5 mg L-1), (3) 2 x 10(6) phagocytes + Hg (50 mu g L-1) and (4) 2 x 10(6) phagocytes + IONP + Hg. Samplings were performed at 0, 2, 4, 8, 16, 24, 48 and 72 h of exposure. A. anguilla P-, G-, HK- and S-phagocytes in vitro exposure to IONP alone revealed either increased (except HK-phagocytes at 16 h) or no change in viability, suggesting that the cells are metabolically active and resistant to IONP exposure alone. In terms of phagocytes overactivation and reactive oxygen species (ROS) production as an indirect mechanism of immunotoxicity, the phagocytes responded in the following manner: P->S->HK-=G-phagocytes for IONP exposure alone, S->HK->P-=G-phagocytes for Hg exposure alone and HK->G-=S->P-phagocytes for concomitant exposure. Overall, considering Hg as a surrogate for metals and its association with IONP, as well as the likelihood that it could pose a serious threat to aquatic organisms by modulating their immune defense mechanisms if accidentally discharged into the aquatic environment, current results suggest that the step of IONP-metal complex removal must not be underrated and should be processed without any more ado. |
format |
Article in Journal/Newspaper |
author |
Costa, Leonor C. Mohmood, Iram Trindade, Tito Saleem, Mohammad Duarte, Armando C. Pereira, Eduarda Ahmad, Iqbal |
author_facet |
Costa, Leonor C. Mohmood, Iram Trindade, Tito Saleem, Mohammad Duarte, Armando C. Pereira, Eduarda Ahmad, Iqbal |
author_sort |
Costa, Leonor C. |
title |
Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.) |
title_short |
Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.) |
title_full |
Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.) |
title_fullStr |
Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.) |
title_full_unstemmed |
Rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in European eel (Anguilla anguilla L.) |
title_sort |
rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in european eel (anguilla anguilla l.) |
publisher |
SPRINGER HEIDELBERG |
publishDate |
2015 |
url |
http://hdl.handle.net/10773/21007 https://doi.org/10.1007/s11356-015-5375-9 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F74410%2F2010/PT info:eu-repo/grantAgreement/FCT/COMPETE/132951/PT info:eu-repo/grantAgreement/FCT/5876-PPCDTI/129903/PT info:eu-repo/grantAgreement/FCT/5876/147332/PT 10.1007/s11356-015-5375-9 0944-1344 http://hdl.handle.net/10773/21007 doi:10.1007/s11356-015-5375-9 |
op_rights |
restrictedAccess |
op_doi |
https://doi.org/10.1007/s11356-015-5375-9 |
container_title |
Environmental Science and Pollution Research |
container_volume |
22 |
container_issue |
23 |
container_start_page |
18574 |
op_container_end_page |
18589 |
_version_ |
1766398751487819776 |