Hydroxyapatite-based materials of marine origin: A bioactivity and sintering study

• Published in: Materials Science and Engineering: C
• Main Authors: Piccirillo, C., Pullar, R. C., Costa, E., Santos-Silva, A., Pintado, M. M. E., Castro, P. M. L.
• Format: Article in Journal/Newspaper
• Language: English
• Published: ELSEVIER SCIENCE BV 2015
• Subjects: BETA-TRICALCIUM PHOSPHATE; IN-VITRO; SUBSTITUTED HYDROXYAPATITE; BONE REPAIR; FISH BONES; SURFACE; OSTEOCONDUCTIVITY; EXTRACTION; CERAMICS; BEHAVIOR; atlantic cod
• Online Access: http://hdl.handle.net/10773/20376; https://doi.org/10.1016/j.msec.2015.03.020

Single phase hydroxyapatite (HAp) and biphasic material hydroxyapatite/beta-tricalcium phosphate (HAp/beta-TCP) were obtained from a marine source (Atlantic cod fish bones). Here we report a study on the biological properties of these materials, including cytotoxicity, bioactivity and haemocompatibility. Results showed that the materials are not cytotoxic, neither in their powder nor in pellet form; indeed growth of Saos-2 cells was comparable to that of commercial. The haemolysis rate was lower than 2%; hence the materials can be classified as non-haemolytic. Moreover, when immersed in Simulated Body Fluid (SBF), crystal formation was observed on the surface of both materials. The sintering behaviour of the samples was also studied; both powders showed very high sinterability (density higher than 95% of the theoretical value). Overall, these results confirm the suitability of these materials for biomedical applications. (C) 2015 Elsevier B.V. All rights reserved.