Oil hydrocarbon-bacteria interactions in coastal environments
Doutoramento em Biologia The ability of microorganisms to use oil hydrocarbons as a source of carbon and energy is crucial for environmental oil detoxification. However, there is still a lack of knowledge on fundamental aspects of this process on specific habitats and under different climate scenari...
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Universidade de Aveiro
2014
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ftria:oai:ria.ua.pt:10773/12248 2023-05-15T17:52:04+02:00 Oil hydrocarbon-bacteria interactions in coastal environments Interacções entre bactérias e hidrocarbonetos de petróleo em ambientes costeiros Coelho, Francisco José Riso da Costa Cunha, Angela Gomes, Newton Carlos Marcial 2014-12-26T17:00:00Z http://hdl.handle.net/10773/12248 eng eng Universidade de Aveiro FCT - PTDC/AAC-CLI/107916/2008 COMPETE – FCOMP-01-0124-FEDER-008657 FCT - SFRH/BD/46322/2008 http://hdl.handle.net/10773/12248 101418299 openAccess Biologia - Teses de doutoramento Hidrocarbonetos - Água do mar Bactérias Petróleo Poluição da água Comunidades microbiológicas doctoralThesis 2014 ftria 2022-05-25T18:34:03Z Doutoramento em Biologia The ability of microorganisms to use oil hydrocarbons as a source of carbon and energy is crucial for environmental oil detoxification. However, there is still a lack of knowledge on fundamental aspects of this process on specific habitats and under different climate scenarios. In the first phase of this work, the culturable fraction of the oil hydrocarbon (OH) degrading bacteria from the sea surface microlayer (SML) of the estuarine system Ria de Aveiro was characterized. In the second phase, the impact of oil contamination on the active bacterial community was studied under climate change scenarios. Pseudomonas emerged as the prevailing genera among OH degrading bacteria in the SML. Moreover, culture-independent methods revealed that the relative abundance and diversity of Gammaproteobacteria, in which Pseudomonas is included, varies along an estuarine gradient of contamination. In order to access the impact of oil contamination on microbial communities under climate change scenarios, an experimental life support system for microcosm experiments (ELLS) was developed and validated for simulation of climate change effects on microbial communities. With the ELSS it is possible to simulate, in controlled conditions, fundamental parameters of the dynamics of coastal and estuarine systems while maintaining community structure in terms of the abundance of the most relevant members of the indigenous bacterial community. A microcosm experiment in which the independent and combined impact of ultraviolet radiation, ocean acidification and oil contamination on microbial communities was conducted. The impact on bacterial communities was accessed with a 16S RNA (cDNA) based barcode pyrosequencing approach. There was a drastic decrease of Desulfobacterales relative abundance after oil contamination under the reduced pH value estimated for 2100, when compared to present values. Since members of this order are known OH degraders, such a significant decrease may have consequences on OH detoxification ... Doctoral or Postdoctoral Thesis Ocean acidification Repositório Institucional da Universidade de Aveiro (RIA) |
institution |
Open Polar |
collection |
Repositório Institucional da Universidade de Aveiro (RIA) |
op_collection_id |
ftria |
language |
English |
topic |
Biologia - Teses de doutoramento Hidrocarbonetos - Água do mar Bactérias Petróleo Poluição da água Comunidades microbiológicas |
spellingShingle |
Biologia - Teses de doutoramento Hidrocarbonetos - Água do mar Bactérias Petróleo Poluição da água Comunidades microbiológicas Coelho, Francisco José Riso da Costa Oil hydrocarbon-bacteria interactions in coastal environments |
topic_facet |
Biologia - Teses de doutoramento Hidrocarbonetos - Água do mar Bactérias Petróleo Poluição da água Comunidades microbiológicas |
description |
Doutoramento em Biologia The ability of microorganisms to use oil hydrocarbons as a source of carbon and energy is crucial for environmental oil detoxification. However, there is still a lack of knowledge on fundamental aspects of this process on specific habitats and under different climate scenarios. In the first phase of this work, the culturable fraction of the oil hydrocarbon (OH) degrading bacteria from the sea surface microlayer (SML) of the estuarine system Ria de Aveiro was characterized. In the second phase, the impact of oil contamination on the active bacterial community was studied under climate change scenarios. Pseudomonas emerged as the prevailing genera among OH degrading bacteria in the SML. Moreover, culture-independent methods revealed that the relative abundance and diversity of Gammaproteobacteria, in which Pseudomonas is included, varies along an estuarine gradient of contamination. In order to access the impact of oil contamination on microbial communities under climate change scenarios, an experimental life support system for microcosm experiments (ELLS) was developed and validated for simulation of climate change effects on microbial communities. With the ELSS it is possible to simulate, in controlled conditions, fundamental parameters of the dynamics of coastal and estuarine systems while maintaining community structure in terms of the abundance of the most relevant members of the indigenous bacterial community. A microcosm experiment in which the independent and combined impact of ultraviolet radiation, ocean acidification and oil contamination on microbial communities was conducted. The impact on bacterial communities was accessed with a 16S RNA (cDNA) based barcode pyrosequencing approach. There was a drastic decrease of Desulfobacterales relative abundance after oil contamination under the reduced pH value estimated for 2100, when compared to present values. Since members of this order are known OH degraders, such a significant decrease may have consequences on OH detoxification ... |
author2 |
Cunha, Angela Gomes, Newton Carlos Marcial |
format |
Doctoral or Postdoctoral Thesis |
author |
Coelho, Francisco José Riso da Costa |
author_facet |
Coelho, Francisco José Riso da Costa |
author_sort |
Coelho, Francisco José Riso da Costa |
title |
Oil hydrocarbon-bacteria interactions in coastal environments |
title_short |
Oil hydrocarbon-bacteria interactions in coastal environments |
title_full |
Oil hydrocarbon-bacteria interactions in coastal environments |
title_fullStr |
Oil hydrocarbon-bacteria interactions in coastal environments |
title_full_unstemmed |
Oil hydrocarbon-bacteria interactions in coastal environments |
title_sort |
oil hydrocarbon-bacteria interactions in coastal environments |
publisher |
Universidade de Aveiro |
publishDate |
2014 |
url |
http://hdl.handle.net/10773/12248 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
FCT - PTDC/AAC-CLI/107916/2008 COMPETE – FCOMP-01-0124-FEDER-008657 FCT - SFRH/BD/46322/2008 http://hdl.handle.net/10773/12248 101418299 |
op_rights |
openAccess |
_version_ |
1766159382290104320 |