Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica
"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants...
Published in: | Process Biochemistry |
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Elsevier Science LTD
2009
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Online Access: | http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1474 |
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ftrepnacmexico:oai:ipicyt.repositorioinstitucional.mx:1010/1474 2023-05-15T13:42:07+02:00 Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica SERGIO ANGEL GARCIA ECHAURI LEANDRO GABRIEL ORDONEZ ACEVEDO JUAN ANTONIO ROJAS CONTRERAS ANA PAULINA BARBA DE LA ROSA ANTONIO DE LEON RODRIGUEZ 2009-09 application/pdf http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1474 unknown Elsevier Science LTD info:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1016/j.procbio.2009.04.021 citation:Sergio A. García Echauri, Manuel Gidekel, Ana Gutiérrez Moraga, Leandro G. Ordóñez, Juan A. Rojas Contreras, Ana P. Barba de la Rosa, Antonio De León Rodríguez, Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica, Process Biochemistry, Volume 44, Issue 9, 2009, Pages 969-974. http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1474 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND info:eu-repo/classification/Autor/Antioxidant info:eu-repo/classification/Autor/Plant info:eu-repo/classification/Autor/Extremophil info:eu-repo/classification/Autor/Psychrophilic enzyme info:eu-repo/classification/Autor/Photo-oxidation info:eu-repo/classification/Autor/Oxidative stress info:eu-repo/classification/cti/6 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 2009 ftrepnacmexico https://doi.org/10.1016/j.procbio.2009.04.021 2021-07-02T06:29:17Z "Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 °C. However, zymogram test showed that the enzyme has more activity at 4 °C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods." Article in Journal/Newspaper Antarc* Antarctica Repositorio Nacional Gobierno de Mexico Process Biochemistry 44 9 969 974 |
institution |
Open Polar |
collection |
Repositorio Nacional Gobierno de Mexico |
op_collection_id |
ftrepnacmexico |
language |
unknown |
topic |
info:eu-repo/classification/Autor/Antioxidant info:eu-repo/classification/Autor/Plant info:eu-repo/classification/Autor/Extremophil info:eu-repo/classification/Autor/Psychrophilic enzyme info:eu-repo/classification/Autor/Photo-oxidation info:eu-repo/classification/Autor/Oxidative stress info:eu-repo/classification/cti/6 |
spellingShingle |
info:eu-repo/classification/Autor/Antioxidant info:eu-repo/classification/Autor/Plant info:eu-repo/classification/Autor/Extremophil info:eu-repo/classification/Autor/Psychrophilic enzyme info:eu-repo/classification/Autor/Photo-oxidation info:eu-repo/classification/Autor/Oxidative stress info:eu-repo/classification/cti/6 SERGIO ANGEL GARCIA ECHAURI LEANDRO GABRIEL ORDONEZ ACEVEDO JUAN ANTONIO ROJAS CONTRERAS ANA PAULINA BARBA DE LA ROSA ANTONIO DE LEON RODRIGUEZ Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica |
topic_facet |
info:eu-repo/classification/Autor/Antioxidant info:eu-repo/classification/Autor/Plant info:eu-repo/classification/Autor/Extremophil info:eu-repo/classification/Autor/Psychrophilic enzyme info:eu-repo/classification/Autor/Photo-oxidation info:eu-repo/classification/Autor/Oxidative stress info:eu-repo/classification/cti/6 |
description |
"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 °C. However, zymogram test showed that the enzyme has more activity at 4 °C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods." |
format |
Article in Journal/Newspaper |
author |
SERGIO ANGEL GARCIA ECHAURI LEANDRO GABRIEL ORDONEZ ACEVEDO JUAN ANTONIO ROJAS CONTRERAS ANA PAULINA BARBA DE LA ROSA ANTONIO DE LEON RODRIGUEZ |
author_facet |
SERGIO ANGEL GARCIA ECHAURI LEANDRO GABRIEL ORDONEZ ACEVEDO JUAN ANTONIO ROJAS CONTRERAS ANA PAULINA BARBA DE LA ROSA ANTONIO DE LEON RODRIGUEZ |
author_sort |
SERGIO ANGEL GARCIA ECHAURI |
title |
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica |
title_short |
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica |
title_full |
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica |
title_fullStr |
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica |
title_full_unstemmed |
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica |
title_sort |
heterologous expression of a novel psychrophilic cu/zn superoxide dismutase from deschampsia antarctica |
publisher |
Elsevier Science LTD |
publishDate |
2009 |
url |
http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1474 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1016/j.procbio.2009.04.021 citation:Sergio A. García Echauri, Manuel Gidekel, Ana Gutiérrez Moraga, Leandro G. Ordóñez, Juan A. Rojas Contreras, Ana P. Barba de la Rosa, Antonio De León Rodríguez, Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica, Process Biochemistry, Volume 44, Issue 9, 2009, Pages 969-974. http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1474 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.procbio.2009.04.021 |
container_title |
Process Biochemistry |
container_volume |
44 |
container_issue |
9 |
container_start_page |
969 |
op_container_end_page |
974 |
_version_ |
1766162772867940352 |