Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro
"Mollusk shell is composed of two CaCO3 polymorphs (calcite and aragonite) and an organic matrix that consists of acetic acid- or ethylenediaminetetraacetic acid (EDTA)-soluble and insoluble proteins and other biomolecules (polysaccharides, β-chitin). However, the Shell matrix proteins involved...
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2020
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ftrepnacmexico:oai:cibnor.repositorioinstitucional.mx:1001/1980 2023-05-15T15:58:40+02:00 Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro RAQUEL GABRIELA ARROYO LORANCA NORMA YOLANDA HERNANDEZ SAAVEDRA LUIS HERNANDEZ ADAME Crisalejandra Rivera Pérez 2020 application/pdf http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1980 eng eng Public Library of Science info:eu-repo/semantics/reference/DOI/DOI:10.1371/journal.pone.0230431 info:eu-repo/semantics/reference/ISSN/ISSN: 1932-6203 info:eu-repo/semantics/reference/URL/URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230431 citation:Arroyo-Loranca RG, Hernandez-Saavedra NY, Hernandez-Adame L, Rivera-Perez C (2020) Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro. PLoS ONE 15(3): e0230431. https://doi.org/ 10.1371/journal.pone.0230431 http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1980 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND PLoS ONE info:eu-repo/classification/AUTOR/Pteria sterna Mollusk shell info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2401 info:eu-repo/classification/cti/240113 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftrepnacmexico https://doi.org/10.1371/journal.pone.0230431 2021-07-02T07:05:43Z "Mollusk shell is composed of two CaCO3 polymorphs (calcite and aragonite) and an organic matrix that consists of acetic acid- or ethylenediaminetetraacetic acid (EDTA)-soluble and insoluble proteins and other biomolecules (polysaccharides, β-chitin). However, the Shell matrix proteins involved in nacre formation are not fully known. Thus, the aim of this study was to identify and characterize a novel protein from the acetic acid-insoluble fraction from the shell of Pteria sterna, named in this study as Ps19, to have a better understanding of the biomineralization process. Ps19 biochemical characterization showed that it is a glycoprotein that exhibits calcium- and chitin-binding capabilities. Additionally, it is capable of inducing aragonite plate crystallization in vitro. Ps19 partial peptide sequence showed similarity with other known shell matrix proteins, but it displayed similarity with proteins from Crassostrea gigas, Mizuhopecten yessoensis, Biomphalaria glabrata, Alpysia californica, Lottia gigantea and Elysia chlorotica. The results obtained indicated that Ps19 might play an important role in nacre growth of mollusk shells." Article in Journal/Newspaper Crassostrea gigas Repositorio Nacional Gobierno de Mexico PLOS ONE 15 3 e0230431 |
institution |
Open Polar |
collection |
Repositorio Nacional Gobierno de Mexico |
op_collection_id |
ftrepnacmexico |
language |
English |
topic |
info:eu-repo/classification/AUTOR/Pteria sterna Mollusk shell info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2401 info:eu-repo/classification/cti/240113 |
spellingShingle |
info:eu-repo/classification/AUTOR/Pteria sterna Mollusk shell info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2401 info:eu-repo/classification/cti/240113 RAQUEL GABRIELA ARROYO LORANCA NORMA YOLANDA HERNANDEZ SAAVEDRA LUIS HERNANDEZ ADAME Crisalejandra Rivera Pérez Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
topic_facet |
info:eu-repo/classification/AUTOR/Pteria sterna Mollusk shell info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2401 info:eu-repo/classification/cti/240113 |
description |
"Mollusk shell is composed of two CaCO3 polymorphs (calcite and aragonite) and an organic matrix that consists of acetic acid- or ethylenediaminetetraacetic acid (EDTA)-soluble and insoluble proteins and other biomolecules (polysaccharides, β-chitin). However, the Shell matrix proteins involved in nacre formation are not fully known. Thus, the aim of this study was to identify and characterize a novel protein from the acetic acid-insoluble fraction from the shell of Pteria sterna, named in this study as Ps19, to have a better understanding of the biomineralization process. Ps19 biochemical characterization showed that it is a glycoprotein that exhibits calcium- and chitin-binding capabilities. Additionally, it is capable of inducing aragonite plate crystallization in vitro. Ps19 partial peptide sequence showed similarity with other known shell matrix proteins, but it displayed similarity with proteins from Crassostrea gigas, Mizuhopecten yessoensis, Biomphalaria glabrata, Alpysia californica, Lottia gigantea and Elysia chlorotica. The results obtained indicated that Ps19 might play an important role in nacre growth of mollusk shells." |
format |
Article in Journal/Newspaper |
author |
RAQUEL GABRIELA ARROYO LORANCA NORMA YOLANDA HERNANDEZ SAAVEDRA LUIS HERNANDEZ ADAME Crisalejandra Rivera Pérez |
author_facet |
RAQUEL GABRIELA ARROYO LORANCA NORMA YOLANDA HERNANDEZ SAAVEDRA LUIS HERNANDEZ ADAME Crisalejandra Rivera Pérez |
author_sort |
RAQUEL GABRIELA ARROYO LORANCA |
title |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_short |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_full |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_fullStr |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_full_unstemmed |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_sort |
ps19, a novel chitin binding protein from pteria sterna capable to mineralize aragonite plates in vitro |
publisher |
Public Library of Science |
publishDate |
2020 |
url |
http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1980 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
PLoS ONE |
op_relation |
info:eu-repo/semantics/reference/DOI/DOI:10.1371/journal.pone.0230431 info:eu-repo/semantics/reference/ISSN/ISSN: 1932-6203 info:eu-repo/semantics/reference/URL/URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230431 citation:Arroyo-Loranca RG, Hernandez-Saavedra NY, Hernandez-Adame L, Rivera-Perez C (2020) Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro. PLoS ONE 15(3): e0230431. https://doi.org/ 10.1371/journal.pone.0230431 http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1980 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1371/journal.pone.0230431 |
container_title |
PLOS ONE |
container_volume |
15 |
container_issue |
3 |
container_start_page |
e0230431 |
_version_ |
1766394441881354240 |