A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species
There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped h...
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ftrepec:oai:RePEc:plo:pone00:0114844 2023-05-15T15:53:51+02:00 A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114844 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114844&type=printable unknown https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114844 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114844&type=printable article ftrepec 2020-12-04T13:35:23Z There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA (“processed fins”). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples). Article in Journal/Newspaper Cetorhinus maximus Lamna nasus Porbeagle RePEc (Research Papers in Economics) |
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There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA (“processed fins”). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples). |
format |
Article in Journal/Newspaper |
author |
Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman |
spellingShingle |
Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
author_facet |
Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman |
author_sort |
Andrew T Fields |
title |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_short |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_full |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_fullStr |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_full_unstemmed |
A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species |
title_sort |
novel mini-dna barcoding assay to identify processed fins from internationally protected shark species |
url |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114844 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114844&type=printable |
genre |
Cetorhinus maximus Lamna nasus Porbeagle |
genre_facet |
Cetorhinus maximus Lamna nasus Porbeagle |
op_relation |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114844 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114844&type=printable |
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1766389030479462400 |