Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus

To efficiently investigate the forage preference of Svalbard reindeer (Rangifer tarandus platyrhynchus), we applied length-heterogeneity polymerase chain reaction (LH-PCR) based on length differences of internal transcribed spacer (ITS) regions of ribosomal RNA (rRNA) to fecal samples from R. tarand...

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Main Authors: Sungbae Joo, Donguk Han, Eun Ju Lee, Sangkyu Park
Format: Article in Journal/Newspaper
Language:unknown
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Online Access:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091552
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0091552&type=printable
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spelling ftrepec:oai:RePEc:plo:pone00:0091552 2024-04-14T08:18:39+00:00 Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus Sungbae Joo Donguk Han Eun Ju Lee Sangkyu Park https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091552 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0091552&type=printable unknown https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091552 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0091552&type=printable article ftrepec 2024-03-19T10:30:36Z To efficiently investigate the forage preference of Svalbard reindeer (Rangifer tarandus platyrhynchus), we applied length-heterogeneity polymerase chain reaction (LH-PCR) based on length differences of internal transcribed spacer (ITS) regions of ribosomal RNA (rRNA) to fecal samples from R. tarandus platyrhynchus. A length-heterogeneity (LH) database was constructed using both collected potential food sources of Svalbard reindeer and fecal samples, followed by PCR, cloning and sequencing. In total, eighteen fecal samples were collected between 2011 and 2012 from 2 geographic regions and 15 samples were successfully amplified by PCR. The LH-PCR analysis detected abundant peaks, 18.6 peaks on an average per sample, ranging from 100 to 500 bp in size and showing distinct patterns associated with both regions and years of sample collection. Principal component analysis (PCA) resulted in clustering of 15 fecal samples into 3 groups by the year of collection and region with a statistically significant difference at 99.9% level. The first 2 principal components (PCs) explained 71.1% of the total variation among the samples. Through comparison with LH database and identification by cloning and sequencing, lichens (Stereocaulon sp. and Ochrolechia sp.) and plant species (Salix polaris and Saxifraga oppositifolia) were detected as the food sources that contributed most to the Svalbard reindeer diet. Our results suggest that the use of LH-PCR analysis would be a non-invasive and efficient monitoring tool for characterizing the foraging strategy of Svalbard reindeer. Additionally, combining sequence information would increase its resolving power in identification of foraged diet components. Article in Journal/Newspaper Rangifer tarandus Rangifer tarandus platyrhynchus Salix polaris Saxifraga oppositifolia Svalbard svalbard reindeer RePEc (Research Papers in Economics) Svalbard
institution Open Polar
collection RePEc (Research Papers in Economics)
op_collection_id ftrepec
language unknown
description To efficiently investigate the forage preference of Svalbard reindeer (Rangifer tarandus platyrhynchus), we applied length-heterogeneity polymerase chain reaction (LH-PCR) based on length differences of internal transcribed spacer (ITS) regions of ribosomal RNA (rRNA) to fecal samples from R. tarandus platyrhynchus. A length-heterogeneity (LH) database was constructed using both collected potential food sources of Svalbard reindeer and fecal samples, followed by PCR, cloning and sequencing. In total, eighteen fecal samples were collected between 2011 and 2012 from 2 geographic regions and 15 samples were successfully amplified by PCR. The LH-PCR analysis detected abundant peaks, 18.6 peaks on an average per sample, ranging from 100 to 500 bp in size and showing distinct patterns associated with both regions and years of sample collection. Principal component analysis (PCA) resulted in clustering of 15 fecal samples into 3 groups by the year of collection and region with a statistically significant difference at 99.9% level. The first 2 principal components (PCs) explained 71.1% of the total variation among the samples. Through comparison with LH database and identification by cloning and sequencing, lichens (Stereocaulon sp. and Ochrolechia sp.) and plant species (Salix polaris and Saxifraga oppositifolia) were detected as the food sources that contributed most to the Svalbard reindeer diet. Our results suggest that the use of LH-PCR analysis would be a non-invasive and efficient monitoring tool for characterizing the foraging strategy of Svalbard reindeer. Additionally, combining sequence information would increase its resolving power in identification of foraged diet components.
format Article in Journal/Newspaper
author Sungbae Joo
Donguk Han
Eun Ju Lee
Sangkyu Park
spellingShingle Sungbae Joo
Donguk Han
Eun Ju Lee
Sangkyu Park
Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus
author_facet Sungbae Joo
Donguk Han
Eun Ju Lee
Sangkyu Park
author_sort Sungbae Joo
title Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus
title_short Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus
title_full Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus
title_fullStr Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus
title_full_unstemmed Use of Length Heterogeneity Polymerase Chain Reaction (LH-PCR) as Non-Invasive Approach for Dietary Analysis of Svalbard Reindeer, Rangifer tarandus platyrhynchus
title_sort use of length heterogeneity polymerase chain reaction (lh-pcr) as non-invasive approach for dietary analysis of svalbard reindeer, rangifer tarandus platyrhynchus
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091552
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0091552&type=printable
geographic Svalbard
geographic_facet Svalbard
genre Rangifer tarandus
Rangifer tarandus platyrhynchus
Salix polaris
Saxifraga oppositifolia
Svalbard
svalbard reindeer
genre_facet Rangifer tarandus
Rangifer tarandus platyrhynchus
Salix polaris
Saxifraga oppositifolia
Svalbard
svalbard reindeer
op_relation https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091552
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0091552&type=printable
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