Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology
Lipases from Candida antarctica B (nonspecific lipase) and Rhizomucor miehei (1,3-specific lipase) were simultaneously immobilized on epoxy functionalized silica gel under mild conditions. The results showed rapid and simple immobilization of 4–15 mg of CALB:RML (different ratios 4:1, 2:1, 1.5:1, 1:...
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ftrepec:oai:RePEc:eee:renene:v:141:y:2019:i:c:p:847-857 2024-04-14T08:01:17+00:00 Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology Shahedi, Mansour Yousefi, Maryam Habibi, Zohreh Mohammadi, Mehdi As'habi, Mohammad Ali http://www.sciencedirect.com/science/article/pii/S0960148119305269 unknown http://www.sciencedirect.com/science/article/pii/S0960148119305269 article ftrepec 2024-03-19T10:28:46Z Lipases from Candida antarctica B (nonspecific lipase) and Rhizomucor miehei (1,3-specific lipase) were simultaneously immobilized on epoxy functionalized silica gel under mild conditions. The results showed rapid and simple immobilization of 4–15 mg of CALB:RML (different ratios 4:1, 2:1, 1.5:1, 1:1) on 1 g of support after 6 h. The thermal stability of derivatives and also their stability in methanol were greatly improved compared to the single immobilized enzyme. All the derivatives were also used to catalyze the transesterification of palm oil with methanol to produce fatty acid methyl esters (FAMEs). Response surface methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of five factors, reaction temperature, methanol/oil ratio, reaction time, t-butanol concentration and CALB:RML ratio on the fatty acid methyl esters (FAME) yield. A quadratic polynomial equation was obtained for methanolysis reaction by multiple regression analysis. The optimum combinations for the reaction were CALB:RML ratio (2.5:1), t-butanol to oil (39.9 wt%), temperature (35.6 °C), methanol:oil ratio (5.9), reaction time 33.5 h. FAME yield of 78.3.5%, which was very close to the predicted value of 75.2%, was obtained. Verification experiment confirmed the validity of the predicted model. Biodiesel; Co-immobilization; Lipase; Response surface methodology; Article in Journal/Newspaper Antarc* Antarctica RePEc (Research Papers in Economics) |
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Open Polar |
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RePEc (Research Papers in Economics) |
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ftrepec |
language |
unknown |
description |
Lipases from Candida antarctica B (nonspecific lipase) and Rhizomucor miehei (1,3-specific lipase) were simultaneously immobilized on epoxy functionalized silica gel under mild conditions. The results showed rapid and simple immobilization of 4–15 mg of CALB:RML (different ratios 4:1, 2:1, 1.5:1, 1:1) on 1 g of support after 6 h. The thermal stability of derivatives and also their stability in methanol were greatly improved compared to the single immobilized enzyme. All the derivatives were also used to catalyze the transesterification of palm oil with methanol to produce fatty acid methyl esters (FAMEs). Response surface methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of five factors, reaction temperature, methanol/oil ratio, reaction time, t-butanol concentration and CALB:RML ratio on the fatty acid methyl esters (FAME) yield. A quadratic polynomial equation was obtained for methanolysis reaction by multiple regression analysis. The optimum combinations for the reaction were CALB:RML ratio (2.5:1), t-butanol to oil (39.9 wt%), temperature (35.6 °C), methanol:oil ratio (5.9), reaction time 33.5 h. FAME yield of 78.3.5%, which was very close to the predicted value of 75.2%, was obtained. Verification experiment confirmed the validity of the predicted model. Biodiesel; Co-immobilization; Lipase; Response surface methodology; |
format |
Article in Journal/Newspaper |
author |
Shahedi, Mansour Yousefi, Maryam Habibi, Zohreh Mohammadi, Mehdi As'habi, Mohammad Ali |
spellingShingle |
Shahedi, Mansour Yousefi, Maryam Habibi, Zohreh Mohammadi, Mehdi As'habi, Mohammad Ali Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology |
author_facet |
Shahedi, Mansour Yousefi, Maryam Habibi, Zohreh Mohammadi, Mehdi As'habi, Mohammad Ali |
author_sort |
Shahedi, Mansour |
title |
Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology |
title_short |
Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology |
title_full |
Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology |
title_fullStr |
Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology |
title_full_unstemmed |
Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology |
title_sort |
co-immobilization of rhizomucor miehei lipase and candida antarctica lipase b and optimization of biocatalytic biodiesel production from palm oil using response surface methodology |
url |
http://www.sciencedirect.com/science/article/pii/S0960148119305269 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0960148119305269 |
_version_ |
1796308239038545920 |