Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)
Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater infows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodel...
Published in: | Journal of Applied Phycology |
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Language: | Croatian |
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2022
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Online Access: | http://fulir.irb.hr/7430/ http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf https://link.springer.com/article/10.1007/s10811-022-02745-8 https://doi.org/10.1007/s10811-022-02745-8 |
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ftrboskovicinst:oai:fulir.irb.hr:7430 2023-05-15T18:18:37+02:00 Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) Vrana Špoljarić, Ivna Bakija Alempijević, Saranda Novosel, Nives Ivošević DeNardis, Nadica Žigon, Dušan Ogrinc, Nives Gašparović, Blaženka 2022 application/pdf http://fulir.irb.hr/7430/ http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf https://link.springer.com/article/10.1007/s10811-022-02745-8 https://doi.org/10.1007/s10811-022-02745-8 hr hrv Springer info:eu-repo/grantAgreement/HRZZ/IP/IP-2018-01-5840 info:eu-repo/grantAgreement/HRZZ/IP/IP-2013-11-8607 http://fulir.irb.hr/7430/ https://link.springer.com/article/10.1007/s10811-022-02745-8 doi:10.1007/s10811-022-02745-8 http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf info:eu-repo/semantics/openAccess Journal of applied phycology Biology Interdisciplinary Natural Sciences Biotechnology info:eu-repo/semantics/article 2022 ftrboskovicinst https://doi.org/10.1007/s10811-022-02745-8 2022-07-08T13:11:26Z Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater infows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identifed 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition. Article in Journal/Newspaper Sea ice Ruđer Bošković Institute Zagreb: Full-text Institutional Repository (FULIR) Journal of Applied Phycology 34 3 1457 1470 |
institution |
Open Polar |
collection |
Ruđer Bošković Institute Zagreb: Full-text Institutional Repository (FULIR) |
op_collection_id |
ftrboskovicinst |
language |
Croatian |
topic |
Biology Interdisciplinary Natural Sciences Biotechnology |
spellingShingle |
Biology Interdisciplinary Natural Sciences Biotechnology Vrana Špoljarić, Ivna Bakija Alempijević, Saranda Novosel, Nives Ivošević DeNardis, Nadica Žigon, Dušan Ogrinc, Nives Gašparović, Blaženka Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) |
topic_facet |
Biology Interdisciplinary Natural Sciences Biotechnology |
description |
Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater infows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identifed 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition. |
format |
Article in Journal/Newspaper |
author |
Vrana Špoljarić, Ivna Bakija Alempijević, Saranda Novosel, Nives Ivošević DeNardis, Nadica Žigon, Dušan Ogrinc, Nives Gašparović, Blaženka |
author_facet |
Vrana Špoljarić, Ivna Bakija Alempijević, Saranda Novosel, Nives Ivošević DeNardis, Nadica Žigon, Dušan Ogrinc, Nives Gašparović, Blaženka |
author_sort |
Vrana Špoljarić, Ivna |
title |
Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) |
title_short |
Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) |
title_full |
Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) |
title_fullStr |
Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) |
title_full_unstemmed |
Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) |
title_sort |
hyposalinity induces significant polar lipid remodeling in the marine microalga dunaliella tertiolecta (chlorophyceae) |
publisher |
Springer |
publishDate |
2022 |
url |
http://fulir.irb.hr/7430/ http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf https://link.springer.com/article/10.1007/s10811-022-02745-8 https://doi.org/10.1007/s10811-022-02745-8 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
Journal of applied phycology |
op_relation |
info:eu-repo/grantAgreement/HRZZ/IP/IP-2018-01-5840 info:eu-repo/grantAgreement/HRZZ/IP/IP-2013-11-8607 http://fulir.irb.hr/7430/ https://link.springer.com/article/10.1007/s10811-022-02745-8 doi:10.1007/s10811-022-02745-8 http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s10811-022-02745-8 |
container_title |
Journal of Applied Phycology |
container_volume |
34 |
container_issue |
3 |
container_start_page |
1457 |
op_container_end_page |
1470 |
_version_ |
1766195249995055104 |