Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater infows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodel...

Full description

Bibliographic Details
Published in:Journal of Applied Phycology
Main Authors: Vrana Špoljarić, Ivna, Bakija Alempijević, Saranda, Novosel, Nives, Ivošević DeNardis, Nadica, Žigon, Dušan, Ogrinc, Nives, Gašparović, Blaženka
Format: Article in Journal/Newspaper
Language:Croatian
Published: Springer 2022
Subjects:
Online Access:http://fulir.irb.hr/7430/
http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf
https://link.springer.com/article/10.1007/s10811-022-02745-8
https://doi.org/10.1007/s10811-022-02745-8
id ftrboskovicinst:oai:fulir.irb.hr:7430
record_format openpolar
spelling ftrboskovicinst:oai:fulir.irb.hr:7430 2023-05-15T18:18:37+02:00 Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae) Vrana Špoljarić, Ivna Bakija Alempijević, Saranda Novosel, Nives Ivošević DeNardis, Nadica Žigon, Dušan Ogrinc, Nives Gašparović, Blaženka 2022 application/pdf http://fulir.irb.hr/7430/ http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf https://link.springer.com/article/10.1007/s10811-022-02745-8 https://doi.org/10.1007/s10811-022-02745-8 hr hrv Springer info:eu-repo/grantAgreement/HRZZ/IP/IP-2018-01-5840 info:eu-repo/grantAgreement/HRZZ/IP/IP-2013-11-8607 http://fulir.irb.hr/7430/ https://link.springer.com/article/10.1007/s10811-022-02745-8 doi:10.1007/s10811-022-02745-8 http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf info:eu-repo/semantics/openAccess Journal of applied phycology Biology Interdisciplinary Natural Sciences Biotechnology info:eu-repo/semantics/article 2022 ftrboskovicinst https://doi.org/10.1007/s10811-022-02745-8 2022-07-08T13:11:26Z Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater infows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identifed 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition. Article in Journal/Newspaper Sea ice Ruđer Bošković Institute Zagreb: Full-text Institutional Repository (FULIR) Journal of Applied Phycology 34 3 1457 1470
institution Open Polar
collection Ruđer Bošković Institute Zagreb: Full-text Institutional Repository (FULIR)
op_collection_id ftrboskovicinst
language Croatian
topic Biology
Interdisciplinary Natural Sciences
Biotechnology
spellingShingle Biology
Interdisciplinary Natural Sciences
Biotechnology
Vrana Špoljarić, Ivna
Bakija Alempijević, Saranda
Novosel, Nives
Ivošević DeNardis, Nadica
Žigon, Dušan
Ogrinc, Nives
Gašparović, Blaženka
Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)
topic_facet Biology
Interdisciplinary Natural Sciences
Biotechnology
description Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater infows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identifed 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition.
format Article in Journal/Newspaper
author Vrana Špoljarić, Ivna
Bakija Alempijević, Saranda
Novosel, Nives
Ivošević DeNardis, Nadica
Žigon, Dušan
Ogrinc, Nives
Gašparović, Blaženka
author_facet Vrana Špoljarić, Ivna
Bakija Alempijević, Saranda
Novosel, Nives
Ivošević DeNardis, Nadica
Žigon, Dušan
Ogrinc, Nives
Gašparović, Blaženka
author_sort Vrana Špoljarić, Ivna
title Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)
title_short Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)
title_full Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)
title_fullStr Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)
title_full_unstemmed Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)
title_sort hyposalinity induces significant polar lipid remodeling in the marine microalga dunaliella tertiolecta (chlorophyceae)
publisher Springer
publishDate 2022
url http://fulir.irb.hr/7430/
http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf
https://link.springer.com/article/10.1007/s10811-022-02745-8
https://doi.org/10.1007/s10811-022-02745-8
genre Sea ice
genre_facet Sea ice
op_source Journal of applied phycology
op_relation info:eu-repo/grantAgreement/HRZZ/IP/IP-2018-01-5840
info:eu-repo/grantAgreement/HRZZ/IP/IP-2013-11-8607
http://fulir.irb.hr/7430/
https://link.springer.com/article/10.1007/s10811-022-02745-8
doi:10.1007/s10811-022-02745-8
http://fulir.irb.hr/7430/1/1193062.13._Vrana_2022.pdf
op_rights info:eu-repo/semantics/openAccess
op_doi https://doi.org/10.1007/s10811-022-02745-8
container_title Journal of Applied Phycology
container_volume 34
container_issue 3
container_start_page 1457
op_container_end_page 1470
_version_ 1766195249995055104