Ice Association in Microbes
Thesis (Ph.D, Biology) -- Queen's University, 2010-09-21 15:58:14.932 Microbes have a remarkable ability to adapt to a host of environmental stressors, including low temperature, high pressure and osmotic stresses. The adaptations of resistant microbes to low temperatures are varied, and may in...
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ftqueensuniv:oai:https://qspace.library.queensu.ca:1974/7479 2024-06-02T07:58:42+00:00 Ice Association in Microbes Wilson, Sandra Biology Walker, Virginia K. 2010-09-21 15:58:14.932 application/pdf http://hdl.handle.net/1974/7479 eng eng Canadian theses http://hdl.handle.net/1974/7479 This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner. Freeze-Thaw Ice Nucleation Activity Ice Recrystallization Inhibition Cross-Tolerance thesis 2010 ftqueensuniv 2024-05-06T10:47:32Z Thesis (Ph.D, Biology) -- Queen's University, 2010-09-21 15:58:14.932 Microbes have a remarkable ability to adapt to a host of environmental stressors, including low temperature, high pressure and osmotic stresses. The adaptations of resistant microbes to low temperatures are varied, and may include the accumulation of solutes to maintain osmotic balance, the production of antifreeze proteins (AFPs) or ice nucleation proteins (INPs) to manipulate ice growth or formation. AFPs depress the freezing point, inhibit ice recrystallization, and have been reported to inhibit or delay the growth of gas hydrates. Conversely, INPs precipitate ice formation at relatively high subzero temperatures. Collectively, these activities can be described as ‘ice-association’ activities. Here, ice-affinity and/or freeze-thaw cycling were used to either select for isolates with ice association properties or to assess the low temperature resistance of microbial consortia derived from various environments. Ice-affinity successfully selected psychrotolerant microbes from cultured temperate and boreal soils, some of which had been previously reported in glaciers and Arctic/Antarctic sites. Many of the recovered microbes demonstrated ice-association activities. Freeze-thaw selection also greatly decreased the abundance and diversity of consortia from distinct sites, and allowed the recovery of individual isolates, many of which demonstrated ice-association. Freeze-thaw selection was also used to assess the role of cross-tolerance between osmotic and freeze-thaw stresses, based on the common challenge of desiccation. Microbial consortia from lakes with varying degrees of salinity were subjected to freeze-thaw stress, and the consortia from more saline lakes tended to show greater low temperature resistance. While few of the recovered microbes demonstrated ice-association activities, those from the more saline lakes tended to contain a higher intracellular solute concentration and were more likely to form biofilms. This underscores the ... Thesis Antarc* Antarctic Arctic Queen's University, Ontario: QSpace Arctic Antarctic |
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Open Polar |
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Queen's University, Ontario: QSpace |
op_collection_id |
ftqueensuniv |
language |
English |
topic |
Freeze-Thaw Ice Nucleation Activity Ice Recrystallization Inhibition Cross-Tolerance |
spellingShingle |
Freeze-Thaw Ice Nucleation Activity Ice Recrystallization Inhibition Cross-Tolerance Wilson, Sandra Ice Association in Microbes |
topic_facet |
Freeze-Thaw Ice Nucleation Activity Ice Recrystallization Inhibition Cross-Tolerance |
description |
Thesis (Ph.D, Biology) -- Queen's University, 2010-09-21 15:58:14.932 Microbes have a remarkable ability to adapt to a host of environmental stressors, including low temperature, high pressure and osmotic stresses. The adaptations of resistant microbes to low temperatures are varied, and may include the accumulation of solutes to maintain osmotic balance, the production of antifreeze proteins (AFPs) or ice nucleation proteins (INPs) to manipulate ice growth or formation. AFPs depress the freezing point, inhibit ice recrystallization, and have been reported to inhibit or delay the growth of gas hydrates. Conversely, INPs precipitate ice formation at relatively high subzero temperatures. Collectively, these activities can be described as ‘ice-association’ activities. Here, ice-affinity and/or freeze-thaw cycling were used to either select for isolates with ice association properties or to assess the low temperature resistance of microbial consortia derived from various environments. Ice-affinity successfully selected psychrotolerant microbes from cultured temperate and boreal soils, some of which had been previously reported in glaciers and Arctic/Antarctic sites. Many of the recovered microbes demonstrated ice-association activities. Freeze-thaw selection also greatly decreased the abundance and diversity of consortia from distinct sites, and allowed the recovery of individual isolates, many of which demonstrated ice-association. Freeze-thaw selection was also used to assess the role of cross-tolerance between osmotic and freeze-thaw stresses, based on the common challenge of desiccation. Microbial consortia from lakes with varying degrees of salinity were subjected to freeze-thaw stress, and the consortia from more saline lakes tended to show greater low temperature resistance. While few of the recovered microbes demonstrated ice-association activities, those from the more saline lakes tended to contain a higher intracellular solute concentration and were more likely to form biofilms. This underscores the ... |
author2 |
Biology Walker, Virginia K. |
format |
Thesis |
author |
Wilson, Sandra |
author_facet |
Wilson, Sandra |
author_sort |
Wilson, Sandra |
title |
Ice Association in Microbes |
title_short |
Ice Association in Microbes |
title_full |
Ice Association in Microbes |
title_fullStr |
Ice Association in Microbes |
title_full_unstemmed |
Ice Association in Microbes |
title_sort |
ice association in microbes |
publishDate |
2010 |
url |
http://hdl.handle.net/1974/7479 |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
Canadian theses http://hdl.handle.net/1974/7479 |
op_rights |
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner. |
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1800742170655719424 |