An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids
In teleost fish, the microbiome plays a variety of roles in host physiology and adaptability to different environments. Anadromous fish (migrating to sea after maturation and returning to freshwater to spawn) are reported to experience turnover of their bacterial communities when transitioning betwe...
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ftqueensuniv:oai:https://qspace.library.queensu.ca:1974/26261 2024-06-02T08:00:41+00:00 An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids Element, Geraint Biology 2019-05-30T21:27:57Z application/pdf http://hdl.handle.net/1974/26261 eng eng Canadian theses http://hdl.handle.net/1974/26261 Queen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canada ProQuest PhD and Master's Theses International Dissemination Agreement Intellectual Property Guidelines at Queen's University Copying and Preserving Your Thesis This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner. Fisheries Microbiomes Arctic thesis 2019 ftqueensuniv 2024-05-06T10:47:33Z In teleost fish, the microbiome plays a variety of roles in host physiology and adaptability to different environments. Anadromous fish (migrating to sea after maturation and returning to freshwater to spawn) are reported to experience turnover of their bacterial communities when transitioning between marine and freshwater environments. Microbiomes of Arctic salmonids are poorly studied, and cold-water environments may support colonization by different microbes. Additionally, anadromous Arctic salmonids perform seasonal migrations, escaping super-cooled seawater in winter. Therefore, it is of interest to determine if these fish experience repeated seasonal recolonizations. This thesis attempts to further understanding of Arctic salmonid microbiomes in two investigations. In the first investigation, anadromous Arctic char (Salvelinus alpinus) from the Kitikmeot region, Nunavut, were sampled and bacterial communities from the intestine characterized using 16S rRNA gene fragment analysis. Bacterial communities were compared across seasonal habitats within the char’s migration route. Communities showed differences between brackish and freshwater habitats, and between different temporal stages of freshwater residence. Brackish communities were broadly consistent with taxa seen in other anadromous salmonids, but were rich in psychrophiles at the genus level, including a putative symbiote, Photobacterium iliopiscarium occurring in >90% of fish from brackish waters. Freshwater communities were more variable and less consistent taxonomically with other salmonids, possibly reflecting char’s winter fasting behaviour. These results suggest that climate change could affect relationships between Arctic char and their symbiotes. In the second investigation, intestinal communities of lake whitefish (Coregonus clupeaformis) were compared to char, with which they were sympatric and behaving anadromously. Lake whitefish appeared to experience similar community shifts with salinity, but community composition was significantly ... Thesis Arctic Climate change Kitikmeot Nunavut Salvelinus alpinus Queen's University, Ontario: QSpace Arctic Nunavut |
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Open Polar |
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Queen's University, Ontario: QSpace |
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ftqueensuniv |
language |
English |
topic |
Fisheries Microbiomes Arctic |
spellingShingle |
Fisheries Microbiomes Arctic Element, Geraint An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids |
topic_facet |
Fisheries Microbiomes Arctic |
description |
In teleost fish, the microbiome plays a variety of roles in host physiology and adaptability to different environments. Anadromous fish (migrating to sea after maturation and returning to freshwater to spawn) are reported to experience turnover of their bacterial communities when transitioning between marine and freshwater environments. Microbiomes of Arctic salmonids are poorly studied, and cold-water environments may support colonization by different microbes. Additionally, anadromous Arctic salmonids perform seasonal migrations, escaping super-cooled seawater in winter. Therefore, it is of interest to determine if these fish experience repeated seasonal recolonizations. This thesis attempts to further understanding of Arctic salmonid microbiomes in two investigations. In the first investigation, anadromous Arctic char (Salvelinus alpinus) from the Kitikmeot region, Nunavut, were sampled and bacterial communities from the intestine characterized using 16S rRNA gene fragment analysis. Bacterial communities were compared across seasonal habitats within the char’s migration route. Communities showed differences between brackish and freshwater habitats, and between different temporal stages of freshwater residence. Brackish communities were broadly consistent with taxa seen in other anadromous salmonids, but were rich in psychrophiles at the genus level, including a putative symbiote, Photobacterium iliopiscarium occurring in >90% of fish from brackish waters. Freshwater communities were more variable and less consistent taxonomically with other salmonids, possibly reflecting char’s winter fasting behaviour. These results suggest that climate change could affect relationships between Arctic char and their symbiotes. In the second investigation, intestinal communities of lake whitefish (Coregonus clupeaformis) were compared to char, with which they were sympatric and behaving anadromously. Lake whitefish appeared to experience similar community shifts with salinity, but community composition was significantly ... |
author2 |
Biology |
format |
Thesis |
author |
Element, Geraint |
author_facet |
Element, Geraint |
author_sort |
Element, Geraint |
title |
An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids |
title_short |
An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids |
title_full |
An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids |
title_fullStr |
An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids |
title_full_unstemmed |
An Exploration of the Intestinal Bacteria of Two Anadromous Arctic Salmonids |
title_sort |
exploration of the intestinal bacteria of two anadromous arctic salmonids |
publishDate |
2019 |
url |
http://hdl.handle.net/1974/26261 |
geographic |
Arctic Nunavut |
geographic_facet |
Arctic Nunavut |
genre |
Arctic Climate change Kitikmeot Nunavut Salvelinus alpinus |
genre_facet |
Arctic Climate change Kitikmeot Nunavut Salvelinus alpinus |
op_relation |
Canadian theses http://hdl.handle.net/1974/26261 |
op_rights |
Queen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canada ProQuest PhD and Master's Theses International Dissemination Agreement Intellectual Property Guidelines at Queen's University Copying and Preserving Your Thesis This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner. |
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1800744795754201088 |