The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus
BACKGROUND: Anguillicola crassus is an economically and ecologically important parasitic nematode of eels. The native range of A. crassus is in East Asia, where it infects Anguilla japonica, the Japanese eel. A. crassus was introduced into European eels, Anguilla anguilla, 30 years ago. The parasite...
Published in: | BMC Genomics |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2013
|
Subjects: | |
Online Access: | https://pure.qub.ac.uk/en/publications/d1bcb18e-444c-4337-9f73-7b67bca6ed1a https://doi.org/10.1186/1471-2164-14-87 |
id |
ftqueensubelpubl:oai:pure.qub.ac.uk/portal:publications/d1bcb18e-444c-4337-9f73-7b67bca6ed1a |
---|---|
record_format |
openpolar |
spelling |
ftqueensubelpubl:oai:pure.qub.ac.uk/portal:publications/d1bcb18e-444c-4337-9f73-7b67bca6ed1a 2024-05-19T07:28:36+00:00 The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus Heitlinger, Emanuel Bridgett, Stephen Montazam, Anna Taraschewski, Horst Blaxter, Mark 2013-02-08 https://pure.qub.ac.uk/en/publications/d1bcb18e-444c-4337-9f73-7b67bca6ed1a https://doi.org/10.1186/1471-2164-14-87 eng eng https://pure.qub.ac.uk/en/publications/d1bcb18e-444c-4337-9f73-7b67bca6ed1a info:eu-repo/semantics/restrictedAccess Heitlinger , E , Bridgett , S , Montazam , A , Taraschewski , H & Blaxter , M 2013 , ' The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus ' , BMC Genomics , vol. 14 , pp. 87 . https://doi.org/10.1186/1471-2164-14-87 Air Sacs/parasitology Animals Contig Mapping Dracunculoidea/classification Energy Metabolism/genetics Female Genes Helminth Introduced Species Male Peptide Hydrolases/genetics Phylogeny Polymorphism Single Nucleotide Protein Sorting Signals/genetics Sequence Analysis RNA Transcriptome article 2013 ftqueensubelpubl https://doi.org/10.1186/1471-2164-14-87 2024-04-25T00:06:28Z BACKGROUND: Anguillicola crassus is an economically and ecologically important parasitic nematode of eels. The native range of A. crassus is in East Asia, where it infects Anguilla japonica, the Japanese eel. A. crassus was introduced into European eels, Anguilla anguilla, 30 years ago. The parasite is more pathogenic in its new host than in its native one, and is thought to threaten the endangered An. anguilla across its range. The molecular bases for the increased pathogenicity of the nematodes in their new hosts is not known. RESULTS: A reference transcriptome was assembled for A. crassus from Roche 454 pyrosequencing data. Raw reads (756,363 total) from nematodes from An. japonica and An. anguilla hosts were filtered for likely host contaminants and ribosomal RNAs. The remaining 353,055 reads were assembled into 11,372 contigs of a high confidence assembly (spanning 6.6 Mb) and an additional 21,153 singletons and contigs of a lower confidence assembly (spanning an additional 6.2 Mb). Roughly 55% of the high confidence assembly contigs were annotated with domain- or protein sequence similarity derived functional information. Sequences conserved only in nematodes, or unique to A. crassus were more likely to have secretory signal peptides. Thousands of high quality single nucleotide polymorphisms were identified, and coding polymorphism was correlated with differential expression between individual nematodes. Transcripts identified as being under positive selection were enriched in peptidases. Enzymes involved in energy metabolism were enriched in the set of genes differentially expressed between European and Asian A. crassus. CONCLUSIONS: The reference transcriptome of A. crassus is of high quality, and will serve as a basis for future work on the invasion biology of this important parasite. The polymorphisms identified will provide a key tool set for analysis of population structure and identification of genes likely to be involved in increased pathogenicity in European eel hosts. The identification of ... Article in Journal/Newspaper Anguilla anguilla European eel Queen's University Belfast Research Portal BMC Genomics 14 1 87 |
institution |
Open Polar |
collection |
Queen's University Belfast Research Portal |
op_collection_id |
ftqueensubelpubl |
language |
English |
topic |
Air Sacs/parasitology Animals Contig Mapping Dracunculoidea/classification Energy Metabolism/genetics Female Genes Helminth Introduced Species Male Peptide Hydrolases/genetics Phylogeny Polymorphism Single Nucleotide Protein Sorting Signals/genetics Sequence Analysis RNA Transcriptome |
spellingShingle |
Air Sacs/parasitology Animals Contig Mapping Dracunculoidea/classification Energy Metabolism/genetics Female Genes Helminth Introduced Species Male Peptide Hydrolases/genetics Phylogeny Polymorphism Single Nucleotide Protein Sorting Signals/genetics Sequence Analysis RNA Transcriptome Heitlinger, Emanuel Bridgett, Stephen Montazam, Anna Taraschewski, Horst Blaxter, Mark The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus |
topic_facet |
Air Sacs/parasitology Animals Contig Mapping Dracunculoidea/classification Energy Metabolism/genetics Female Genes Helminth Introduced Species Male Peptide Hydrolases/genetics Phylogeny Polymorphism Single Nucleotide Protein Sorting Signals/genetics Sequence Analysis RNA Transcriptome |
description |
BACKGROUND: Anguillicola crassus is an economically and ecologically important parasitic nematode of eels. The native range of A. crassus is in East Asia, where it infects Anguilla japonica, the Japanese eel. A. crassus was introduced into European eels, Anguilla anguilla, 30 years ago. The parasite is more pathogenic in its new host than in its native one, and is thought to threaten the endangered An. anguilla across its range. The molecular bases for the increased pathogenicity of the nematodes in their new hosts is not known. RESULTS: A reference transcriptome was assembled for A. crassus from Roche 454 pyrosequencing data. Raw reads (756,363 total) from nematodes from An. japonica and An. anguilla hosts were filtered for likely host contaminants and ribosomal RNAs. The remaining 353,055 reads were assembled into 11,372 contigs of a high confidence assembly (spanning 6.6 Mb) and an additional 21,153 singletons and contigs of a lower confidence assembly (spanning an additional 6.2 Mb). Roughly 55% of the high confidence assembly contigs were annotated with domain- or protein sequence similarity derived functional information. Sequences conserved only in nematodes, or unique to A. crassus were more likely to have secretory signal peptides. Thousands of high quality single nucleotide polymorphisms were identified, and coding polymorphism was correlated with differential expression between individual nematodes. Transcripts identified as being under positive selection were enriched in peptidases. Enzymes involved in energy metabolism were enriched in the set of genes differentially expressed between European and Asian A. crassus. CONCLUSIONS: The reference transcriptome of A. crassus is of high quality, and will serve as a basis for future work on the invasion biology of this important parasite. The polymorphisms identified will provide a key tool set for analysis of population structure and identification of genes likely to be involved in increased pathogenicity in European eel hosts. The identification of ... |
format |
Article in Journal/Newspaper |
author |
Heitlinger, Emanuel Bridgett, Stephen Montazam, Anna Taraschewski, Horst Blaxter, Mark |
author_facet |
Heitlinger, Emanuel Bridgett, Stephen Montazam, Anna Taraschewski, Horst Blaxter, Mark |
author_sort |
Heitlinger, Emanuel |
title |
The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus |
title_short |
The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus |
title_full |
The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus |
title_fullStr |
The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus |
title_full_unstemmed |
The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus |
title_sort |
transcriptome of the invasive eel swimbladder nematode parasite anguillicola crassus |
publishDate |
2013 |
url |
https://pure.qub.ac.uk/en/publications/d1bcb18e-444c-4337-9f73-7b67bca6ed1a https://doi.org/10.1186/1471-2164-14-87 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Heitlinger , E , Bridgett , S , Montazam , A , Taraschewski , H & Blaxter , M 2013 , ' The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus ' , BMC Genomics , vol. 14 , pp. 87 . https://doi.org/10.1186/1471-2164-14-87 |
op_relation |
https://pure.qub.ac.uk/en/publications/d1bcb18e-444c-4337-9f73-7b67bca6ed1a |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1186/1471-2164-14-87 |
container_title |
BMC Genomics |
container_volume |
14 |
container_issue |
1 |
container_start_page |
87 |
_version_ |
1799474787410509824 |