Development of type I genetic markers from expressed sequence tags in highly polymorphic species

• Published in: Marine Biotechnology
• Main Authors: Kim, Woo-Jin, Jung, Hyungtaek, Gaffney, Patrick
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Springer New York 2011
• Subjects: EST; Indel; Marker; SNP; UTR; Pacific; Indel’; Crassostrea gigas; Pacific oyster
• Online Access: https://eprints.qut.edu.au/92882/

Expressed sequence tag (EST) databases provide a primary source of nuclear DNA sequences for genetic marker development in non-model organisms. To date, the process has been relatively inefficient for several reasons: - 1) priming site polymorphism in the template leads to inferior or erratic amplif...