Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways
Amoebic gill disease (AGD) is a parasite-mediated proliferative gill disease capable of affecting a range of teleost hosts. While a moderate heritability for AGD resistance in Atlantic salmon has been reported previously, the mechanisms by which individuals resist the proliferative effects remain po...
Published in: | Developmental & Comparative Immunology |
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ftqueensland:oai:eprints.qut.edu.au:79857 2024-02-04T09:58:55+01:00 Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways Wynne, James O'Sullivan, Maree Stone, Glenn Cook, Mathew Nowak, Barbara Lovell, David Taylor, Richard Elliott, Nicholas 2008 https://eprints.qut.edu.au/79857/ unknown Elsevier Ltd. doi:10.1016/j.dci.2008.05.013 Wynne, James, O'Sullivan, Maree, Stone, Glenn, Cook, Mathew, Nowak, Barbara, Lovell, David, Taylor, Richard, & Elliott, Nicholas (2008) Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways. Developmental and Comparative Immunology, 32(12), pp. 1539-1560. https://eprints.qut.edu.au/79857/ Science & Engineering Faculty; School of Electrical Engineering & Computer Science Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au Developmental and Comparative Immunology Acquired immunity Amebiasis Animalia Animals Cell Cycle Disease Susceptibility Disease resistance Female Fish Diseases Genetic Gills Host-Parasite Interactions Immunity Innate Lobosea Male Microarray Parasite infection Salmo salar Salmonid Salmonidae Teleostei Transcription amoebic gill disease article cell cycle regulation cell metabolism disease predisposition gene cluster gene expression profiling genetic transcription histopathology immune response microarray analysis nonhuman nucleotide sequence parasitosis priority journal reverse transcription polymerase chain reaction salmon signal transduction transcriptome Contribution to Journal 2008 ftqueensland https://doi.org/10.1016/j.dci.2008.05.013 2024-01-08T23:34:41Z Amoebic gill disease (AGD) is a parasite-mediated proliferative gill disease capable of affecting a range of teleost hosts. While a moderate heritability for AGD resistance in Atlantic salmon has been reported previously, the mechanisms by which individuals resist the proliferative effects remain poorly understood. To gain more knowledge of this commercially important trait, we compared gill transcriptomes of two groups of Atlantic salmon, one designated putatively resistant, and one designated putatively susceptible to AGD. Utilising a 17k Atlantic salmon cDNA microarray we identified 196 transcripts that were differentially expressed between the two groups. Expression of 11 transcripts were further examined with real-time quantitative RT-PCR (qPCR) in the AGD-resistant and AGD-susceptible animals, as well as non-infected naïve fish. Gene expression determined by qPCR was in strong agreement with the microarray analysis. A large number of differentially expressed genes were involved in immune and cell cycle responses. Resistant individuals displayed significantly higher expression of genes involved in adaptive immunity and negative regulation of the cell cycle. In contrast, AGD-susceptible individuals showed higher expression of acute phase proteins and positive regulators of the cell cycle. Combined with the gill histopathology, our results suggest AGD resistance is acquired rather than innately present, and that this resistance is for the most part associated with the dysregulation of immune and cell cycle pathways. © 2008 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Atlantic salmon Salmo salar Queensland University of Technology: QUT ePrints Developmental & Comparative Immunology 32 12 1539 1560 |
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Queensland University of Technology: QUT ePrints |
op_collection_id |
ftqueensland |
language |
unknown |
topic |
Acquired immunity Amebiasis Animalia Animals Cell Cycle Disease Susceptibility Disease resistance Female Fish Diseases Genetic Gills Host-Parasite Interactions Immunity Innate Lobosea Male Microarray Parasite infection Salmo salar Salmonid Salmonidae Teleostei Transcription amoebic gill disease article cell cycle regulation cell metabolism disease predisposition gene cluster gene expression profiling genetic transcription histopathology immune response microarray analysis nonhuman nucleotide sequence parasitosis priority journal reverse transcription polymerase chain reaction salmon signal transduction transcriptome |
spellingShingle |
Acquired immunity Amebiasis Animalia Animals Cell Cycle Disease Susceptibility Disease resistance Female Fish Diseases Genetic Gills Host-Parasite Interactions Immunity Innate Lobosea Male Microarray Parasite infection Salmo salar Salmonid Salmonidae Teleostei Transcription amoebic gill disease article cell cycle regulation cell metabolism disease predisposition gene cluster gene expression profiling genetic transcription histopathology immune response microarray analysis nonhuman nucleotide sequence parasitosis priority journal reverse transcription polymerase chain reaction salmon signal transduction transcriptome Wynne, James O'Sullivan, Maree Stone, Glenn Cook, Mathew Nowak, Barbara Lovell, David Taylor, Richard Elliott, Nicholas Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
topic_facet |
Acquired immunity Amebiasis Animalia Animals Cell Cycle Disease Susceptibility Disease resistance Female Fish Diseases Genetic Gills Host-Parasite Interactions Immunity Innate Lobosea Male Microarray Parasite infection Salmo salar Salmonid Salmonidae Teleostei Transcription amoebic gill disease article cell cycle regulation cell metabolism disease predisposition gene cluster gene expression profiling genetic transcription histopathology immune response microarray analysis nonhuman nucleotide sequence parasitosis priority journal reverse transcription polymerase chain reaction salmon signal transduction transcriptome |
description |
Amoebic gill disease (AGD) is a parasite-mediated proliferative gill disease capable of affecting a range of teleost hosts. While a moderate heritability for AGD resistance in Atlantic salmon has been reported previously, the mechanisms by which individuals resist the proliferative effects remain poorly understood. To gain more knowledge of this commercially important trait, we compared gill transcriptomes of two groups of Atlantic salmon, one designated putatively resistant, and one designated putatively susceptible to AGD. Utilising a 17k Atlantic salmon cDNA microarray we identified 196 transcripts that were differentially expressed between the two groups. Expression of 11 transcripts were further examined with real-time quantitative RT-PCR (qPCR) in the AGD-resistant and AGD-susceptible animals, as well as non-infected naïve fish. Gene expression determined by qPCR was in strong agreement with the microarray analysis. A large number of differentially expressed genes were involved in immune and cell cycle responses. Resistant individuals displayed significantly higher expression of genes involved in adaptive immunity and negative regulation of the cell cycle. In contrast, AGD-susceptible individuals showed higher expression of acute phase proteins and positive regulators of the cell cycle. Combined with the gill histopathology, our results suggest AGD resistance is acquired rather than innately present, and that this resistance is for the most part associated with the dysregulation of immune and cell cycle pathways. © 2008 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Wynne, James O'Sullivan, Maree Stone, Glenn Cook, Mathew Nowak, Barbara Lovell, David Taylor, Richard Elliott, Nicholas |
author_facet |
Wynne, James O'Sullivan, Maree Stone, Glenn Cook, Mathew Nowak, Barbara Lovell, David Taylor, Richard Elliott, Nicholas |
author_sort |
Wynne, James |
title |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_short |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_full |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_fullStr |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_full_unstemmed |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_sort |
resistance to amoebic gill disease (agd) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
publisher |
Elsevier Ltd. |
publishDate |
2008 |
url |
https://eprints.qut.edu.au/79857/ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Developmental and Comparative Immunology |
op_relation |
doi:10.1016/j.dci.2008.05.013 Wynne, James, O'Sullivan, Maree, Stone, Glenn, Cook, Mathew, Nowak, Barbara, Lovell, David, Taylor, Richard, & Elliott, Nicholas (2008) Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways. Developmental and Comparative Immunology, 32(12), pp. 1539-1560. https://eprints.qut.edu.au/79857/ Science & Engineering Faculty; School of Electrical Engineering & Computer Science |
op_rights |
Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
op_doi |
https://doi.org/10.1016/j.dci.2008.05.013 |
container_title |
Developmental & Comparative Immunology |
container_volume |
32 |
container_issue |
12 |
container_start_page |
1539 |
op_container_end_page |
1560 |
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1789963505969922048 |