A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum
Cold-active lipases are of significant interest as biocatalysts in industrial processes. We have identified a lipase that displayed activity towards long carbon-chain-p-nitrophenyl substrates (C12–C18) at 25 °C from the culture supernatant of an Antarctic Penicillium expansum strain assigned P. expa...
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ftqueensland:oai:eprints.qut.edu.au:71179 2024-01-28T10:00:39+01:00 A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum Mohammed, S Te'o, Valentino Nevalainen, H 2013 https://eprints.qut.edu.au/71179/ unknown Springer doi:10.1007/s00294-013-0394-x Mohammed, S, Te'o, Valentino, & Nevalainen, H (2013) A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum. Current Genetics, 59(3), pp. 129-137. https://eprints.qut.edu.au/71179/ Science & Engineering Faculty; School of Earth, Environmental & Biological Sciences Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au Current Genetics Antartic Cold-active lipase Genomic walking PCR Penicillium expansion Contribution to Journal 2013 ftqueensland https://doi.org/10.1007/s00294-013-0394-x 2024-01-01T23:22:43Z Cold-active lipases are of significant interest as biocatalysts in industrial processes. We have identified a lipase that displayed activity towards long carbon-chain-p-nitrophenyl substrates (C12–C18) at 25 °C from the culture supernatant of an Antarctic Penicillium expansum strain assigned P. expansum SM3. Zymography revealed a protein band of around 30 kDa with activity towards olive oil. DNA fragments of a lipase gene designated as lipPE were isolated from the genomic DNA of P. expansum SM3 by genomic walking PCR. Subsequently, the complete genomic lipPE gene was amplified using gene-specific primers designed from the 5′- and 3′-regions. Reverse transcription PCR was used to amplify the lipPE cDNA. The deduced amino acid sequence consisted of 285 residues that included a predicted signal peptide. Three peptides identified by LC/MS/MS analysis of the proteins in the culture supernatant of P. expansum were also present in the deduced amino acid sequence of the lipPE gene suggesting that this gene encoded the lipase identified by initial zymogram activity analysis. Full analysis of the nucleotide and the deduced amino acid sequences indicated that the lipPE gene encodes a novel P. expansum lipase. The lipPE gene was expressed in E. coli for further characterization of the enzyme with a view of assessing its suitability for industrial applications. Article in Journal/Newspaper Antarc* Antarctic antartic* Queensland University of Technology: QUT ePrints Antarctic Current Genetics 59 3 129 137 |
institution |
Open Polar |
collection |
Queensland University of Technology: QUT ePrints |
op_collection_id |
ftqueensland |
language |
unknown |
topic |
Antartic Cold-active lipase Genomic walking PCR Penicillium expansion |
spellingShingle |
Antartic Cold-active lipase Genomic walking PCR Penicillium expansion Mohammed, S Te'o, Valentino Nevalainen, H A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum |
topic_facet |
Antartic Cold-active lipase Genomic walking PCR Penicillium expansion |
description |
Cold-active lipases are of significant interest as biocatalysts in industrial processes. We have identified a lipase that displayed activity towards long carbon-chain-p-nitrophenyl substrates (C12–C18) at 25 °C from the culture supernatant of an Antarctic Penicillium expansum strain assigned P. expansum SM3. Zymography revealed a protein band of around 30 kDa with activity towards olive oil. DNA fragments of a lipase gene designated as lipPE were isolated from the genomic DNA of P. expansum SM3 by genomic walking PCR. Subsequently, the complete genomic lipPE gene was amplified using gene-specific primers designed from the 5′- and 3′-regions. Reverse transcription PCR was used to amplify the lipPE cDNA. The deduced amino acid sequence consisted of 285 residues that included a predicted signal peptide. Three peptides identified by LC/MS/MS analysis of the proteins in the culture supernatant of P. expansum were also present in the deduced amino acid sequence of the lipPE gene suggesting that this gene encoded the lipase identified by initial zymogram activity analysis. Full analysis of the nucleotide and the deduced amino acid sequences indicated that the lipPE gene encodes a novel P. expansum lipase. The lipPE gene was expressed in E. coli for further characterization of the enzyme with a view of assessing its suitability for industrial applications. |
format |
Article in Journal/Newspaper |
author |
Mohammed, S Te'o, Valentino Nevalainen, H |
author_facet |
Mohammed, S Te'o, Valentino Nevalainen, H |
author_sort |
Mohammed, S |
title |
A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum |
title_short |
A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum |
title_full |
A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum |
title_fullStr |
A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum |
title_full_unstemmed |
A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum |
title_sort |
gene encoding a new cold-active lipase from an antarctic isolate of penicillium expansum |
publisher |
Springer |
publishDate |
2013 |
url |
https://eprints.qut.edu.au/71179/ |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic antartic* |
genre_facet |
Antarc* Antarctic antartic* |
op_source |
Current Genetics |
op_relation |
doi:10.1007/s00294-013-0394-x Mohammed, S, Te'o, Valentino, & Nevalainen, H (2013) A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum. Current Genetics, 59(3), pp. 129-137. https://eprints.qut.edu.au/71179/ Science & Engineering Faculty; School of Earth, Environmental & Biological Sciences |
op_rights |
Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
op_doi |
https://doi.org/10.1007/s00294-013-0394-x |
container_title |
Current Genetics |
container_volume |
59 |
container_issue |
3 |
container_start_page |
129 |
op_container_end_page |
137 |
_version_ |
1789325134438334464 |