An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic
Signatories to the Antarctic Treaty System’s Environmental Protocol are committed to preventing incursions of non-native species into Antarctica, but systematic surveillance is rare. Environmental DNA (eDNA) methods provide new opportunities for enhancing detection of non-native species and biosecur...
Published in: | Management of Biological Invasions |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
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Regional Euro-Asian Biological Invasions Centre
2023
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Online Access: | https://eprints.qut.edu.au/248098/ |
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ftqueensland:oai:eprints.qut.edu.au:248098 |
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institution |
Open Polar |
collection |
Queensland University of Technology: QUT ePrints |
op_collection_id |
ftqueensland |
language |
unknown |
topic |
biofouling environmental DNA marine non-native species risk assessment Southern Ocean terrestrial |
spellingShingle |
biofouling environmental DNA marine non-native species risk assessment Southern Ocean terrestrial Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. Ammon, Ulla von Wilson, Nerida G. Zaiko, Anastasija Macdonald, Anna J. An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
topic_facet |
biofouling environmental DNA marine non-native species risk assessment Southern Ocean terrestrial |
description |
Signatories to the Antarctic Treaty System’s Environmental Protocol are committed to preventing incursions of non-native species into Antarctica, but systematic surveillance is rare. Environmental DNA (eDNA) methods provide new opportunities for enhancing detection of non-native species and biosecurity monitoring. To be effective for Antarctic biosecurity, eDNA tests must have appropriate sensitivity and specificity to distinguish non-native from native Antarctic species, and be fit-for-purpose. This requires knowledge of the priority risk species or taxonomic groups for which eDNA surveillance will be informative, validated eDNA assays for those species or groups, and reference DNA sequences for both target non-native and related native Antarctic species. Here, we used an expert elicitation process and decision-by-consensus approach to identify and assess priority biosecurity risks for the Australian Antarctic Program (AAP) in East Antarctica, including identifying high priority non-native species and their potential transport pathways. We determined that the priority targets for biosecurity monitoring were not individual species, but rather broader taxonomic groups such as mussels (Mytilus species), tunicates (Ascidiacea), springtails (Collembola), and grasses (Poaceae). These groups each include multiple species with high risks of introduction to and/or establishment in Antarctica. The most appropriate eDNA methods for the AAP must be capable of detecting a range of species within these high-risk groups (e.g., eDNA metabarcoding). We conclude that the most beneficial Antarctic eDNA biosecurity applications include surveillance of marine species in nearshore environments, terrestrial invertebrates, and biofouling species on vessels visiting Antarctica. An urgent need exists to identify suitable genetic markers for detecting priority species groups, establish baseline terrestrial and marine biodiversity for Antarctic stations, and develop eDNA sampling methods for detecting biofouling organisms. |
format |
Article in Journal/Newspaper |
author |
Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. Ammon, Ulla von Wilson, Nerida G. Zaiko, Anastasija Macdonald, Anna J. |
author_facet |
Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. Ammon, Ulla von Wilson, Nerida G. Zaiko, Anastasija Macdonald, Anna J. |
author_sort |
Clarke, Laurence J. |
title |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_short |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_full |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_fullStr |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_full_unstemmed |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_sort |
expert-driven framework for applying edna tools to improve biosecurity in the antarctic |
publisher |
Regional Euro-Asian Biological Invasions Centre |
publishDate |
2023 |
url |
https://eprints.qut.edu.au/248098/ |
genre |
Antarc* Antarctic Antarctica Australian Antarctic Program East Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Australian Antarctic Program East Antarctica Southern Ocean |
op_source |
Management of Biological Invasions |
op_relation |
https://eprints.qut.edu.au/248098/1/167124929.pdf doi:10.3391/mbi.2023.14.3.01 Clarke, Laurence J., Shaw, Justine D., Suter, Leonie, Atalah, Javier, Bergstrom, Dana M., Biersma, Elisabeth, Convey, Peter, Greve, Michelle, Holland, Oakes, Houghton, Melissa J., Hughes, Kevin A., Johnston, Emma L., King, Catherine K., McCarthy, Arlie H., McGaughran, Angela, Pertierra, Luis R., Robinson, Sharon A., Sherman, Craig D.H., Stark, Jonathan S., Stevens, Mark I., Strugnell, Jan M., Ammon, Ulla von, Wilson, Nerida G., Zaiko, Anastasija, & Macdonald, Anna J. (2023) An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic. Management of Biological Invasions, 14(3), pp. 379-402. http://purl.org/au-research/grants/arc/SR200100005 http://purl.org/au-research/grants/arc/T3_P028 http://purl.org/au-research/grants/arc/T2-P023 http://purl.org/au-research/grants/arc/T2-P016 http://purl.org/au-research/grants/arc/T2-P036 https://eprints.qut.edu.au/248098/ Faculty of Science; School of Biology & Environmental Science |
op_rights |
free_to_read http://creativecommons.org/licenses/by/4.0/ 2023 The Authors This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
op_doi |
https://doi.org/10.3391/mbi.2023.14.3.01 |
container_title |
Management of Biological Invasions |
container_volume |
14 |
container_issue |
3 |
container_start_page |
379 |
op_container_end_page |
402 |
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1799469246315495424 |
spelling |
ftqueensland:oai:eprints.qut.edu.au:248098 2024-05-19T07:31:23+00:00 An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. Ammon, Ulla von Wilson, Nerida G. Zaiko, Anastasija Macdonald, Anna J. 2023-09 application/pdf https://eprints.qut.edu.au/248098/ unknown Regional Euro-Asian Biological Invasions Centre https://eprints.qut.edu.au/248098/1/167124929.pdf doi:10.3391/mbi.2023.14.3.01 Clarke, Laurence J., Shaw, Justine D., Suter, Leonie, Atalah, Javier, Bergstrom, Dana M., Biersma, Elisabeth, Convey, Peter, Greve, Michelle, Holland, Oakes, Houghton, Melissa J., Hughes, Kevin A., Johnston, Emma L., King, Catherine K., McCarthy, Arlie H., McGaughran, Angela, Pertierra, Luis R., Robinson, Sharon A., Sherman, Craig D.H., Stark, Jonathan S., Stevens, Mark I., Strugnell, Jan M., Ammon, Ulla von, Wilson, Nerida G., Zaiko, Anastasija, & Macdonald, Anna J. (2023) An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic. Management of Biological Invasions, 14(3), pp. 379-402. http://purl.org/au-research/grants/arc/SR200100005 http://purl.org/au-research/grants/arc/T3_P028 http://purl.org/au-research/grants/arc/T2-P023 http://purl.org/au-research/grants/arc/T2-P016 http://purl.org/au-research/grants/arc/T2-P036 https://eprints.qut.edu.au/248098/ Faculty of Science; School of Biology & Environmental Science free_to_read http://creativecommons.org/licenses/by/4.0/ 2023 The Authors This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au Management of Biological Invasions biofouling environmental DNA marine non-native species risk assessment Southern Ocean terrestrial Contribution to Journal 2023 ftqueensland https://doi.org/10.3391/mbi.2023.14.3.01 2024-04-24T00:09:08Z Signatories to the Antarctic Treaty System’s Environmental Protocol are committed to preventing incursions of non-native species into Antarctica, but systematic surveillance is rare. Environmental DNA (eDNA) methods provide new opportunities for enhancing detection of non-native species and biosecurity monitoring. To be effective for Antarctic biosecurity, eDNA tests must have appropriate sensitivity and specificity to distinguish non-native from native Antarctic species, and be fit-for-purpose. This requires knowledge of the priority risk species or taxonomic groups for which eDNA surveillance will be informative, validated eDNA assays for those species or groups, and reference DNA sequences for both target non-native and related native Antarctic species. Here, we used an expert elicitation process and decision-by-consensus approach to identify and assess priority biosecurity risks for the Australian Antarctic Program (AAP) in East Antarctica, including identifying high priority non-native species and their potential transport pathways. We determined that the priority targets for biosecurity monitoring were not individual species, but rather broader taxonomic groups such as mussels (Mytilus species), tunicates (Ascidiacea), springtails (Collembola), and grasses (Poaceae). These groups each include multiple species with high risks of introduction to and/or establishment in Antarctica. The most appropriate eDNA methods for the AAP must be capable of detecting a range of species within these high-risk groups (e.g., eDNA metabarcoding). We conclude that the most beneficial Antarctic eDNA biosecurity applications include surveillance of marine species in nearshore environments, terrestrial invertebrates, and biofouling species on vessels visiting Antarctica. An urgent need exists to identify suitable genetic markers for detecting priority species groups, establish baseline terrestrial and marine biodiversity for Antarctic stations, and develop eDNA sampling methods for detecting biofouling organisms. Article in Journal/Newspaper Antarc* Antarctic Antarctica Australian Antarctic Program East Antarctica Southern Ocean Queensland University of Technology: QUT ePrints Management of Biological Invasions 14 3 379 402 |