Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.)
Between July 2011 and May 2012, 1 6 pooled faecal samples were collected on eight occasions (40 fish/occasion) from Atlantic salmon fed two commercial diets in a farm in Tasmania, Australia. From a second Tasmanian farm, 12 pooled faecal samples were collected on three occasions (80 fish/occasion) f...
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ftqueensland:oai:eprints.qut.edu.au:230554 2023-06-18T03:39:50+02:00 Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.) Neuman, C. Hatje, E. Bowman, J. P. Katouli, M. 2014-11-01 https://eprints.qut.edu.au/230554/ unknown New Century Health Publishers Neuman, C., Hatje, E., Bowman, J. P., & Katouli, M. (2014) Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.). International Journal of Probiotics & Prebiotics, 9(4), pp. 119-127. https://eprints.qut.edu.au/230554/ Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au International Journal of Probiotics & Prebiotics LACTIC acid bacteria ATLANTIC salmon PEDIOCOCCUS acidilactici ACID-forming bacteria CYTOTOXINS Pediococcus acidilatici Weissella hellenica Contribution to Journal 2014 ftqueensland 2023-06-05T23:52:42Z Between July 2011 and May 2012, 1 6 pooled faecal samples were collected on eight occasions (40 fish/occasion) from Atlantic salmon fed two commercial diets in a farm in Tasmania, Australia. From a second Tasmanian farm, 12 pooled faecal samples were collected on three occasions (80 fish/occasion) from salmon fed four trial diets. From these samples a total of 160 lactic acid bacteria (LAB) were isolated on MRS agar. Strains were initially typed using PhP-LB plates (PhPlate, AB) and divided into common types (CTs). Strains which belonged to the same CT and found on numerous sampling occasions (n=73) were identified using 16S rRNA sequencing and tested for cytotoxins production, bacteriocins, adherence to Atlantic salmon kidney cells as well as competitively exclude 16 bacterial strains in vitro. These strains were also tested for their resistance against nine antibiotics commonly used in Aquaculture. The 73 LAB strains belonged to six species namely Enterococcus casseliflavus, E. faecalis, E. faecium, Pediococcus acidilactici, and Weisella hellenica of which, three different strains of W. hellenica and one P. acidilactici met most criteria including medium to high adherence and pathogen exclusion capability in vitro. The potential of these strains to serve as probiotic candidates is discussed. Article in Journal/Newspaper Atlantic salmon Salmo salar Queensland University of Technology: QUT ePrints |
institution |
Open Polar |
collection |
Queensland University of Technology: QUT ePrints |
op_collection_id |
ftqueensland |
language |
unknown |
topic |
LACTIC acid bacteria ATLANTIC salmon PEDIOCOCCUS acidilactici ACID-forming bacteria CYTOTOXINS Pediococcus acidilatici Weissella hellenica |
spellingShingle |
LACTIC acid bacteria ATLANTIC salmon PEDIOCOCCUS acidilactici ACID-forming bacteria CYTOTOXINS Pediococcus acidilatici Weissella hellenica Neuman, C. Hatje, E. Bowman, J. P. Katouli, M. Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.) |
topic_facet |
LACTIC acid bacteria ATLANTIC salmon PEDIOCOCCUS acidilactici ACID-forming bacteria CYTOTOXINS Pediococcus acidilatici Weissella hellenica |
description |
Between July 2011 and May 2012, 1 6 pooled faecal samples were collected on eight occasions (40 fish/occasion) from Atlantic salmon fed two commercial diets in a farm in Tasmania, Australia. From a second Tasmanian farm, 12 pooled faecal samples were collected on three occasions (80 fish/occasion) from salmon fed four trial diets. From these samples a total of 160 lactic acid bacteria (LAB) were isolated on MRS agar. Strains were initially typed using PhP-LB plates (PhPlate, AB) and divided into common types (CTs). Strains which belonged to the same CT and found on numerous sampling occasions (n=73) were identified using 16S rRNA sequencing and tested for cytotoxins production, bacteriocins, adherence to Atlantic salmon kidney cells as well as competitively exclude 16 bacterial strains in vitro. These strains were also tested for their resistance against nine antibiotics commonly used in Aquaculture. The 73 LAB strains belonged to six species namely Enterococcus casseliflavus, E. faecalis, E. faecium, Pediococcus acidilactici, and Weisella hellenica of which, three different strains of W. hellenica and one P. acidilactici met most criteria including medium to high adherence and pathogen exclusion capability in vitro. The potential of these strains to serve as probiotic candidates is discussed. |
format |
Article in Journal/Newspaper |
author |
Neuman, C. Hatje, E. Bowman, J. P. Katouli, M. |
author_facet |
Neuman, C. Hatje, E. Bowman, J. P. Katouli, M. |
author_sort |
Neuman, C. |
title |
Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.) |
title_short |
Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.) |
title_full |
Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.) |
title_fullStr |
Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.) |
title_sort |
characterisation of lactic acid bacteria isolated from the hindgut of farmed tasmanian atlantic salmon (salmo salar l.) |
publisher |
New Century Health Publishers |
publishDate |
2014 |
url |
https://eprints.qut.edu.au/230554/ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
International Journal of Probiotics & Prebiotics |
op_relation |
Neuman, C., Hatje, E., Bowman, J. P., & Katouli, M. (2014) Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.). International Journal of Probiotics & Prebiotics, 9(4), pp. 119-127. https://eprints.qut.edu.au/230554/ |
op_rights |
Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
_version_ |
1769004627570720768 |