Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov
Three cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing,...
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American Society for Microbiology
2013
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ftqueensland:oai:eprints.qut.edu.au:219869 2024-02-11T09:59:51+01:00 Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov Stride, Megan Polkinghorne, Adam Miller, Terrence Groff, Joseph LaPatra, Scott Nowak, Barbara 2013 application/pdf https://eprints.qut.edu.au/219869/ unknown American Society for Microbiology https://eprints.qut.edu.au/219869/1/68367.pdf doi:10.1128/AEM.02899-12 Stride, Megan, Polkinghorne, Adam, Miller, Terrence, Groff, Joseph, LaPatra, Scott, & Nowak, Barbara (2013) Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov. Applied and Environmental Microbiology, 79(5), pp. 1590-1597. https://eprints.qut.edu.au/219869/ Faculty of Health; Institute of Health and Biomedical Innovation; School of Biomedical Sciences free_to_read Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au Applied and Environmental Microbiology Contribution to Journal 2013 ftqueensland https://doi.org/10.1128/AEM.02899-12 2024-01-22T23:23:19Z Three cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the order Chlamydiales. A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported "Candidatus Piscichlamydia salmonis" (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35 Chlamydia and Chlamydia-like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the order Chlamydiales. Based on these observations, we propose this bacterium of yellowtail kingfish be known as "Candidatus Parilichlamydia carangidicola" and that the new family be known as "Candidatus Parilichlamydiaceae." Article in Journal/Newspaper Arctic charr Arctic Atlantic salmon Queensland University of Technology: QUT ePrints Arctic Applied and Environmental Microbiology 79 5 1590 1597 |
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Queensland University of Technology: QUT ePrints |
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ftqueensland |
language |
unknown |
description |
Three cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the order Chlamydiales. A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported "Candidatus Piscichlamydia salmonis" (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35 Chlamydia and Chlamydia-like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the order Chlamydiales. Based on these observations, we propose this bacterium of yellowtail kingfish be known as "Candidatus Parilichlamydia carangidicola" and that the new family be known as "Candidatus Parilichlamydiaceae." |
format |
Article in Journal/Newspaper |
author |
Stride, Megan Polkinghorne, Adam Miller, Terrence Groff, Joseph LaPatra, Scott Nowak, Barbara |
spellingShingle |
Stride, Megan Polkinghorne, Adam Miller, Terrence Groff, Joseph LaPatra, Scott Nowak, Barbara Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov |
author_facet |
Stride, Megan Polkinghorne, Adam Miller, Terrence Groff, Joseph LaPatra, Scott Nowak, Barbara |
author_sort |
Stride, Megan |
title |
Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov |
title_short |
Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov |
title_full |
Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov |
title_fullStr |
Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov |
title_full_unstemmed |
Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov |
title_sort |
molecular characterization of 'candidatus parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in yellowtail kingfish, seriola lalandi (valenciennes), and the proposal of a new family, 'candidatus parilichlamydiaceae' fam. nov |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
https://eprints.qut.edu.au/219869/ |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Atlantic salmon |
genre_facet |
Arctic charr Arctic Atlantic salmon |
op_source |
Applied and Environmental Microbiology |
op_relation |
https://eprints.qut.edu.au/219869/1/68367.pdf doi:10.1128/AEM.02899-12 Stride, Megan, Polkinghorne, Adam, Miller, Terrence, Groff, Joseph, LaPatra, Scott, & Nowak, Barbara (2013) Molecular characterization of 'Candidatus Parilichlamydia carangidicola,' a novel chlamydia-like epitheliocystis agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, 'Candidatus Parilichlamydiaceae' fam. nov. Applied and Environmental Microbiology, 79(5), pp. 1590-1597. https://eprints.qut.edu.au/219869/ Faculty of Health; Institute of Health and Biomedical Innovation; School of Biomedical Sciences |
op_rights |
free_to_read Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
op_doi |
https://doi.org/10.1128/AEM.02899-12 |
container_title |
Applied and Environmental Microbiology |
container_volume |
79 |
container_issue |
5 |
container_start_page |
1590 |
op_container_end_page |
1597 |
_version_ |
1790595582157389824 |