Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska
We examined patterns in soil microbial community composition across a successional gradient of drained lake basins in the Arctic Coastal Plain. Analysis of 16S rRNA gene sequences revealed that methanogens closely related to Candidatus ‘Methanoflorens stordalenmirensis’ were the dominant archaea, co...
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ftqueensland:oai:eprints.qut.edu.au:200379 2024-02-04T09:56:35+01:00 Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska Kao-Kniffin, J. Woodcroft, B.J. Carver, S.M. Bockheim, J.G. Handelsman, J. Tyson, G.W. Hinkel, K.M. Mueller, C.W. 2015-12 application/pdf https://eprints.qut.edu.au/200379/ unknown Nature Publishing Group https://eprints.qut.edu.au/200379/1/59189352.pdf doi:10.1038/srep18165 Kao-Kniffin, J., Woodcroft, B.J., Carver, S.M., Bockheim, J.G., Handelsman, J., Tyson, G.W., Hinkel, K.M., & Mueller, C.W. (2015) Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska. Scientific Reports, 5, Article number: 18165. https://eprints.qut.edu.au/200379/ free_to_read http://creativecommons.org/licenses/by/4.0/ The Author(s) This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au Scientific Reports Contribution to Journal 2015 ftqueensland https://doi.org/10.1038/srep18165 2024-01-08T23:52:31Z We examined patterns in soil microbial community composition across a successional gradient of drained lake basins in the Arctic Coastal Plain. Analysis of 16S rRNA gene sequences revealed that methanogens closely related to Candidatus ‘Methanoflorens stordalenmirensis’ were the dominant archaea, comprising >50% of the total archaea at most sites, with particularly high levels in the oldest basins and in the top 57 cm of soil (active and transition layers). Bacterial community composition was more diverse, with lineages from OP11, Actinobacteria, Bacteroidetes and Proteobacteria found in high relative abundance across all sites. Notably, microbial composition appeared to converge in the active layer, but transition and permafrost layer communities across the sites were significantly different to one another. Microbial biomass using fatty acid-based analysis indicated that the youngest basins had increased abundances of gram-positive bacteria and saprotrophic fungi at higher soil organic carbon levels, while the oldest basins displayed an increase in only the gram-positive bacteria. While this study showed differences in microbial populations across the sites relevant to basin age, the dominance of Candidatus ‘M. stordalenmirensis’ across the chronosequence indicates the potential for changes in local carbon cycling, depending on how these methanogens and associated microbial communities respond to warming temperatures. Article in Journal/Newspaper Arctic Arctic permafrost Alaska Queensland University of Technology: QUT ePrints Arctic Scientific Reports 5 1 |
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Open Polar |
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Queensland University of Technology: QUT ePrints |
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ftqueensland |
language |
unknown |
description |
We examined patterns in soil microbial community composition across a successional gradient of drained lake basins in the Arctic Coastal Plain. Analysis of 16S rRNA gene sequences revealed that methanogens closely related to Candidatus ‘Methanoflorens stordalenmirensis’ were the dominant archaea, comprising >50% of the total archaea at most sites, with particularly high levels in the oldest basins and in the top 57 cm of soil (active and transition layers). Bacterial community composition was more diverse, with lineages from OP11, Actinobacteria, Bacteroidetes and Proteobacteria found in high relative abundance across all sites. Notably, microbial composition appeared to converge in the active layer, but transition and permafrost layer communities across the sites were significantly different to one another. Microbial biomass using fatty acid-based analysis indicated that the youngest basins had increased abundances of gram-positive bacteria and saprotrophic fungi at higher soil organic carbon levels, while the oldest basins displayed an increase in only the gram-positive bacteria. While this study showed differences in microbial populations across the sites relevant to basin age, the dominance of Candidatus ‘M. stordalenmirensis’ across the chronosequence indicates the potential for changes in local carbon cycling, depending on how these methanogens and associated microbial communities respond to warming temperatures. |
format |
Article in Journal/Newspaper |
author |
Kao-Kniffin, J. Woodcroft, B.J. Carver, S.M. Bockheim, J.G. Handelsman, J. Tyson, G.W. Hinkel, K.M. Mueller, C.W. |
spellingShingle |
Kao-Kniffin, J. Woodcroft, B.J. Carver, S.M. Bockheim, J.G. Handelsman, J. Tyson, G.W. Hinkel, K.M. Mueller, C.W. Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska |
author_facet |
Kao-Kniffin, J. Woodcroft, B.J. Carver, S.M. Bockheim, J.G. Handelsman, J. Tyson, G.W. Hinkel, K.M. Mueller, C.W. |
author_sort |
Kao-Kniffin, J. |
title |
Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska |
title_short |
Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska |
title_full |
Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska |
title_fullStr |
Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska |
title_full_unstemmed |
Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska |
title_sort |
archaeal and bacterial communities across a chronosequence of drained lake basins in arctic alaska |
publisher |
Nature Publishing Group |
publishDate |
2015 |
url |
https://eprints.qut.edu.au/200379/ |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic permafrost Alaska |
genre_facet |
Arctic Arctic permafrost Alaska |
op_source |
Scientific Reports |
op_relation |
https://eprints.qut.edu.au/200379/1/59189352.pdf doi:10.1038/srep18165 Kao-Kniffin, J., Woodcroft, B.J., Carver, S.M., Bockheim, J.G., Handelsman, J., Tyson, G.W., Hinkel, K.M., & Mueller, C.W. (2015) Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic Alaska. Scientific Reports, 5, Article number: 18165. https://eprints.qut.edu.au/200379/ |
op_rights |
free_to_read http://creativecommons.org/licenses/by/4.0/ The Author(s) This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
op_doi |
https://doi.org/10.1038/srep18165 |
container_title |
Scientific Reports |
container_volume |
5 |
container_issue |
1 |
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1789961030111068160 |