Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974).
Nine yellow-pigmented, spherical bacterial strains isolated from a medieval wall painting (strain D7), from indoor air (strains 3, 6, 7, 13C2, 38, 83 and 118) and from an activated-sludge plant (strain Ballarat) were classified by a polyphasic approach. Analyses of the 16S rRNA gene sequences of thr...
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ftqueensland:oai:eprints.qut.edu.au:108492 2024-02-11T09:57:15+01:00 Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). Wieser, Monika Steiner, Ulrike Vybiral, Dietmar Patel, Bharat K.C. Lubitz, Werner Denner, Ewald B.M. K�mpfer, Peter Maszenan, A.M. Seviour, Robert J. Schumann, Peter Radax, Christian Tindall, Brian Busse, Hans-J�rgen 2002 https://eprints.qut.edu.au/108492/ unknown Microbiology Society doi:10.1099/00207713-52-2-629 Wieser, Monika, Steiner, Ulrike, Vybiral, Dietmar, Patel, Bharat K.C., Lubitz, Werner, Denner, Ewald B.M., K�mpfer, Peter, Maszenan, A.M., Seviour, Robert J., Schumann, Peter, Radax, Christian, Tindall, Brian, & Busse, Hans-J�rgen (2002) Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). International Journal of Systematic and Evolutionary Microbiology, 52(2), pp. 629-637. https://eprints.qut.edu.au/108492/ Science & Engineering Faculty Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au International Journal of Systematic and Evolutionary Microbiology Contribution to Journal 2002 ftqueensland https://doi.org/10.1099/00207713-52-2-629 2024-01-15T23:21:54Z Nine yellow-pigmented, spherical bacterial strains isolated from a medieval wall painting (strain D7), from indoor air (strains 3, 6, 7, 13C2, 38, 83 and 118) and from an activated-sludge plant (strain Ballarat) were classified by a polyphasic approach. Analyses of the 16S rRNA gene sequences of three representatives (strains D7, 118 and Ballarat) indicated that they all belong to the genus Micrococcus. The three isolates shared the highest sequence similarities with Micrococcus luteus DSM 20030T (97.9-98%), Micrococcus antarcticus AS 1.2372T (97.9-98.3%) and Micrococcus lylae DSM 20315T (97.5-97.9%). DNA-DNA reassociation studies clearly demonstrated that all nine isolates belong to the species M. luteus. However, neither their chemotaxonomic features nor their physiological and biochemical properties were consistent with those of M. luteus DSM 20030T. In contrast to M. luteus DSM 20030T, all isolates investigated possessed MK-8(H2) as the major respiratory quinone, and strain Ballarat had an A4alpha peptidoglycan type. On the basis of analyses of their Fourier transform-infrared spectroscopy spectra, isolates D7, 3, 6, 7, 13C2, 38, 83 and 118 could be grouped into a single cluster separate from M. luteus DSM 20030T, strain Ballarat and M. lylae DSM 20315T. In addition, all these isolates could be distinguished from M. luteus DSM 20030T by their ability to assimilate D-maltose, D-trehalose, DL-3-hydroxybutyrate, DL-lactate, pyruvate and L-histidine and to hydrolyse casein. Strains D7, 3, 6, 7, 13C2, 38, 83 and 118 differed from both M. luteus DSM 20030T and strain Ballarat by their ability to assimilate acetate, L-phenylalanine, L-serine and phenylacetate. Furthermore, REP-PCR fingerprinting yielded one common band for these strains, whereas this band was not observed for M. luteus DSM 20030T, strain Ballarat or M. lylae DSM 20315T. On the basis of these data, the species M. luteus can be divided into three biovars that are distinguished by several chemotaxonomic and biochemical traits: biovar I, represented by ... Article in Journal/Newspaper Antarc* antarcticus Queensland University of Technology: QUT ePrints International Journal of Systematic and Evolutionary Microbiology 52 2 629 637 |
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collection |
Queensland University of Technology: QUT ePrints |
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ftqueensland |
language |
unknown |
description |
Nine yellow-pigmented, spherical bacterial strains isolated from a medieval wall painting (strain D7), from indoor air (strains 3, 6, 7, 13C2, 38, 83 and 118) and from an activated-sludge plant (strain Ballarat) were classified by a polyphasic approach. Analyses of the 16S rRNA gene sequences of three representatives (strains D7, 118 and Ballarat) indicated that they all belong to the genus Micrococcus. The three isolates shared the highest sequence similarities with Micrococcus luteus DSM 20030T (97.9-98%), Micrococcus antarcticus AS 1.2372T (97.9-98.3%) and Micrococcus lylae DSM 20315T (97.5-97.9%). DNA-DNA reassociation studies clearly demonstrated that all nine isolates belong to the species M. luteus. However, neither their chemotaxonomic features nor their physiological and biochemical properties were consistent with those of M. luteus DSM 20030T. In contrast to M. luteus DSM 20030T, all isolates investigated possessed MK-8(H2) as the major respiratory quinone, and strain Ballarat had an A4alpha peptidoglycan type. On the basis of analyses of their Fourier transform-infrared spectroscopy spectra, isolates D7, 3, 6, 7, 13C2, 38, 83 and 118 could be grouped into a single cluster separate from M. luteus DSM 20030T, strain Ballarat and M. lylae DSM 20315T. In addition, all these isolates could be distinguished from M. luteus DSM 20030T by their ability to assimilate D-maltose, D-trehalose, DL-3-hydroxybutyrate, DL-lactate, pyruvate and L-histidine and to hydrolyse casein. Strains D7, 3, 6, 7, 13C2, 38, 83 and 118 differed from both M. luteus DSM 20030T and strain Ballarat by their ability to assimilate acetate, L-phenylalanine, L-serine and phenylacetate. Furthermore, REP-PCR fingerprinting yielded one common band for these strains, whereas this band was not observed for M. luteus DSM 20030T, strain Ballarat or M. lylae DSM 20315T. On the basis of these data, the species M. luteus can be divided into three biovars that are distinguished by several chemotaxonomic and biochemical traits: biovar I, represented by ... |
format |
Article in Journal/Newspaper |
author |
Wieser, Monika Steiner, Ulrike Vybiral, Dietmar Patel, Bharat K.C. Lubitz, Werner Denner, Ewald B.M. K�mpfer, Peter Maszenan, A.M. Seviour, Robert J. Schumann, Peter Radax, Christian Tindall, Brian Busse, Hans-J�rgen |
spellingShingle |
Wieser, Monika Steiner, Ulrike Vybiral, Dietmar Patel, Bharat K.C. Lubitz, Werner Denner, Ewald B.M. K�mpfer, Peter Maszenan, A.M. Seviour, Robert J. Schumann, Peter Radax, Christian Tindall, Brian Busse, Hans-J�rgen Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). |
author_facet |
Wieser, Monika Steiner, Ulrike Vybiral, Dietmar Patel, Bharat K.C. Lubitz, Werner Denner, Ewald B.M. K�mpfer, Peter Maszenan, A.M. Seviour, Robert J. Schumann, Peter Radax, Christian Tindall, Brian Busse, Hans-J�rgen |
author_sort |
Wieser, Monika |
title |
Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). |
title_short |
Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). |
title_full |
Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). |
title_fullStr |
Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). |
title_full_unstemmed |
Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). |
title_sort |
emended descriptions of the genus micrococcus, micrococcus luteus (cohn 1872) and micrococcus lylae (kloos et al. 1974). |
publisher |
Microbiology Society |
publishDate |
2002 |
url |
https://eprints.qut.edu.au/108492/ |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
International Journal of Systematic and Evolutionary Microbiology |
op_relation |
doi:10.1099/00207713-52-2-629 Wieser, Monika, Steiner, Ulrike, Vybiral, Dietmar, Patel, Bharat K.C., Lubitz, Werner, Denner, Ewald B.M., K�mpfer, Peter, Maszenan, A.M., Seviour, Robert J., Schumann, Peter, Radax, Christian, Tindall, Brian, & Busse, Hans-J�rgen (2002) Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). International Journal of Systematic and Evolutionary Microbiology, 52(2), pp. 629-637. https://eprints.qut.edu.au/108492/ Science & Engineering Faculty |
op_rights |
Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
op_doi |
https://doi.org/10.1099/00207713-52-2-629 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
52 |
container_issue |
2 |
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629 |
op_container_end_page |
637 |
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