eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED
Environmental DNA (eDNA) is organismal DNA found in nature. It has emerged as a practical way to measure species distribution and abundance in their habitat. Because eDNA is transported in the environment, knowing where it originates remains a challenge, particularly in flowing waters. eDNA is a het...
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ftpurdueunivport:oai:figshare.com:article/17124548 2023-05-15T15:33:04+02:00 eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED Kush Paliwal 2021-12-04T04:23:35Z https://doi.org/10.25394/pgs.17124548.v1 unknown https://figshare.com/articles/thesis/eDNA_TRANSPORT_IN_STREAMS_WITH_COARSE_SEDIMENT_BED/17124548 doi:10.25394/pgs.17124548.v1 CC BY 4.0 CC-BY Water resources engineering Civil engineering not elsewhere classified eDNA transport transient storage retention resuspension breakthrough curve Text Thesis 2021 ftpurdueunivport https://doi.org/10.25394/pgs.17124548.v1 2023-01-18T13:23:06Z Environmental DNA (eDNA) is organismal DNA found in nature. It has emerged as a practical way to measure species distribution and abundance in their habitat. Because eDNA is transported in the environment, knowing where it originates remains a challenge, particularly in flowing waters. eDNA is a heterogeneous mixture of particulate organic matter that settles out of the water column and can be trapped in or near the bed sediment. eDNA data collected during experiments or surveys have a large amount of unexplained variability, making it hard to constrain eDNA transport models. The two guiding questions we answer in this thesis are: 1) Can we predict eDNA transport patterns in a flowing system? and 2) Can we explain the observed variability in eDNA data by the interfacial processes between the water column and the sediment bed? We conducted three experiments in an artificial laboratory channel. In the first experiment, we measured eDNA retention, i.e., the distance eDNA travels downstream of an injection on average. In the second experiment, we compared the observed breakthrough behavior to the predicted pattern from the first experiment results. Finally, in the last experiment, we loaded the streambed with eDNA prior to reproducing the second experiment. For the first experiment (retention), we injected known quantities of Atlantic Salmon (Salmo Salar) and sampled the water column at five locations during the plateau phase. We measured the retention rate as the slope of the (logged) concentration data vs downstream distance and the travel distance as its inverse. The measured travel distance in our small channel (30 cm wide) with a coarse sediment bed (1 cm d50), low flow (1 L/s) and shallow water column (10 cm) was 18.51 m (15.38 – 23.24 m, ± SE). We used the measured retention rate to predict the breakthrough behavior with a simple transport model and showed good agreement between the predicted and the observed concentrations measured in the second experiment. The results from the third experiment had the same ... Thesis Atlantic salmon Salmo salar Purdue University Graduate School: Figshare |
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Open Polar |
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Purdue University Graduate School: Figshare |
op_collection_id |
ftpurdueunivport |
language |
unknown |
topic |
Water resources engineering Civil engineering not elsewhere classified eDNA transport transient storage retention resuspension breakthrough curve |
spellingShingle |
Water resources engineering Civil engineering not elsewhere classified eDNA transport transient storage retention resuspension breakthrough curve Kush Paliwal eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED |
topic_facet |
Water resources engineering Civil engineering not elsewhere classified eDNA transport transient storage retention resuspension breakthrough curve |
description |
Environmental DNA (eDNA) is organismal DNA found in nature. It has emerged as a practical way to measure species distribution and abundance in their habitat. Because eDNA is transported in the environment, knowing where it originates remains a challenge, particularly in flowing waters. eDNA is a heterogeneous mixture of particulate organic matter that settles out of the water column and can be trapped in or near the bed sediment. eDNA data collected during experiments or surveys have a large amount of unexplained variability, making it hard to constrain eDNA transport models. The two guiding questions we answer in this thesis are: 1) Can we predict eDNA transport patterns in a flowing system? and 2) Can we explain the observed variability in eDNA data by the interfacial processes between the water column and the sediment bed? We conducted three experiments in an artificial laboratory channel. In the first experiment, we measured eDNA retention, i.e., the distance eDNA travels downstream of an injection on average. In the second experiment, we compared the observed breakthrough behavior to the predicted pattern from the first experiment results. Finally, in the last experiment, we loaded the streambed with eDNA prior to reproducing the second experiment. For the first experiment (retention), we injected known quantities of Atlantic Salmon (Salmo Salar) and sampled the water column at five locations during the plateau phase. We measured the retention rate as the slope of the (logged) concentration data vs downstream distance and the travel distance as its inverse. The measured travel distance in our small channel (30 cm wide) with a coarse sediment bed (1 cm d50), low flow (1 L/s) and shallow water column (10 cm) was 18.51 m (15.38 – 23.24 m, ± SE). We used the measured retention rate to predict the breakthrough behavior with a simple transport model and showed good agreement between the predicted and the observed concentrations measured in the second experiment. The results from the third experiment had the same ... |
format |
Thesis |
author |
Kush Paliwal |
author_facet |
Kush Paliwal |
author_sort |
Kush Paliwal |
title |
eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED |
title_short |
eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED |
title_full |
eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED |
title_fullStr |
eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED |
title_full_unstemmed |
eDNA TRANSPORT IN STREAMS WITH COARSE SEDIMENT BED |
title_sort |
edna transport in streams with coarse sediment bed |
publishDate |
2021 |
url |
https://doi.org/10.25394/pgs.17124548.v1 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://figshare.com/articles/thesis/eDNA_TRANSPORT_IN_STREAMS_WITH_COARSE_SEDIMENT_BED/17124548 doi:10.25394/pgs.17124548.v1 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.25394/pgs.17124548.v1 |
_version_ |
1766363531509235712 |