Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx

Phenotypic plasticity (genotype × environment interaction) is an especially important means for sessile organisms to cope with environmental variation. While kelps, the globally most productive group of seaweeds, generally possess a wide thermal performance range, kelp populations at their warm dist...

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Main Authors: Daniel Liesner (8983001), Lisa N. S. Shama (6102515), Nora Diehl (8983004), Klaus Valentin (139492), Inka Bartsch (6942989)
Format: Dataset
Language:unknown
Published: 2020
Subjects:
Online Access:https://doi.org/10.3389/fmars.2020.00456.s001
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record_format openpolar
spelling ftpurdueuniv:oai:figshare.com:article/12495749 2023-05-15T15:19:08+02:00 Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx Daniel Liesner (8983001) Lisa N. S. Shama (6102515) Nora Diehl (8983004) Klaus Valentin (139492) Inka Bartsch (6942989) 2020-06-17T04:11:26Z https://doi.org/10.3389/fmars.2020.00456.s001 unknown https://figshare.com/articles/Data_Sheet_1_Thermal_Plasticity_of_the_Kelp_Laminaria_digitata_Phaeophyceae_Across_Life_Cycle_Stages_Reveals_the_Importance_of_Cold_Seasons_for_Marine_Forests_docx/12495749 doi:10.3389/fmars.2020.00456.s001 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering phenotypic plasticity temperature acclimation developmental plasticity carry-over effect biphasic life cycle gametogenesis ontogeny genetic variation Dataset 2020 ftpurdueuniv https://doi.org/10.3389/fmars.2020.00456.s001 2020-06-25T10:35:08Z Phenotypic plasticity (genotype × environment interaction) is an especially important means for sessile organisms to cope with environmental variation. While kelps, the globally most productive group of seaweeds, generally possess a wide thermal performance range, kelp populations at their warm distribution limits are threatened by ocean warming. Here, we investigated effects of temperature during ontogeny of the kelp Laminaria digitata across haploid gametophyte and diploid sporophyte life cycle stages in five distinct genetic lines. We hypothesized that thermal plasticity increases trait performance of juvenile sporophytes in experimental temperatures that match the temperature experienced during gametogenesis and recruitment, and that plasticity differs among genetic lines (genetic variation for plasticity). We applied a full-factorial experimental design to generate different temperature histories by applying 5 and 15°C during meiospore germination, gametogenesis of parental gametophytes and recruitment of offspring sporophytes (19–26 days), and juvenile sporophyte rearing (91–122 days). We then tested for thermal plasticity among temperature history treatments at 5 and 15°C in a final 12-day experiment assessing growth, the storage compound mannitol, carbon and nitrogen contents, and fluorometric responses in 3–4 month old sporophytes for five genetic lines. Our study provides evidence for the importance of cold temperatures at early development on later sporophyte performance of L. digitata. Gametogenesis and recruitment at 5°C promoted higher growth of offspring sporophytes across experimental temperatures. While photosynthetic capacity was higher at 15°C, carbon and nitrogen storage were higher at 5°C, both showing fast acclimation responses. We identified an important role of genetic variation for plasticity in shaping L. digitata’s thermal plasticity. Trait performance at 5 or 15°C (reaction norm slopes) differed among genetic lines, even showing opposite response patterns. Interestingly, genetic variation for plasticity was only significant when sporophytes were reared at 5°C. Thus, we provide evidence that the cold-temperate to Arctic kelp species, L. digitata, which possesses a wide temperature tolerance between 0 and 23°C, is impaired by warm temperature during gametogenesis and recruitment, reducing growth of juvenile sporophytes and expression of variable thermal plasticity in the wild. Dataset Arctic Purdue University: e-Pubs Arctic
institution Open Polar
collection Purdue University: e-Pubs
op_collection_id ftpurdueuniv
language unknown
topic Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
phenotypic plasticity
temperature acclimation
developmental plasticity
carry-over effect
biphasic life cycle
gametogenesis
ontogeny
genetic variation
spellingShingle Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
phenotypic plasticity
temperature acclimation
developmental plasticity
carry-over effect
biphasic life cycle
gametogenesis
ontogeny
genetic variation
Daniel Liesner (8983001)
Lisa N. S. Shama (6102515)
Nora Diehl (8983004)
Klaus Valentin (139492)
Inka Bartsch (6942989)
Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx
topic_facet Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
phenotypic plasticity
temperature acclimation
developmental plasticity
carry-over effect
biphasic life cycle
gametogenesis
ontogeny
genetic variation
description Phenotypic plasticity (genotype × environment interaction) is an especially important means for sessile organisms to cope with environmental variation. While kelps, the globally most productive group of seaweeds, generally possess a wide thermal performance range, kelp populations at their warm distribution limits are threatened by ocean warming. Here, we investigated effects of temperature during ontogeny of the kelp Laminaria digitata across haploid gametophyte and diploid sporophyte life cycle stages in five distinct genetic lines. We hypothesized that thermal plasticity increases trait performance of juvenile sporophytes in experimental temperatures that match the temperature experienced during gametogenesis and recruitment, and that plasticity differs among genetic lines (genetic variation for plasticity). We applied a full-factorial experimental design to generate different temperature histories by applying 5 and 15°C during meiospore germination, gametogenesis of parental gametophytes and recruitment of offspring sporophytes (19–26 days), and juvenile sporophyte rearing (91–122 days). We then tested for thermal plasticity among temperature history treatments at 5 and 15°C in a final 12-day experiment assessing growth, the storage compound mannitol, carbon and nitrogen contents, and fluorometric responses in 3–4 month old sporophytes for five genetic lines. Our study provides evidence for the importance of cold temperatures at early development on later sporophyte performance of L. digitata. Gametogenesis and recruitment at 5°C promoted higher growth of offspring sporophytes across experimental temperatures. While photosynthetic capacity was higher at 15°C, carbon and nitrogen storage were higher at 5°C, both showing fast acclimation responses. We identified an important role of genetic variation for plasticity in shaping L. digitata’s thermal plasticity. Trait performance at 5 or 15°C (reaction norm slopes) differed among genetic lines, even showing opposite response patterns. Interestingly, genetic variation for plasticity was only significant when sporophytes were reared at 5°C. Thus, we provide evidence that the cold-temperate to Arctic kelp species, L. digitata, which possesses a wide temperature tolerance between 0 and 23°C, is impaired by warm temperature during gametogenesis and recruitment, reducing growth of juvenile sporophytes and expression of variable thermal plasticity in the wild.
format Dataset
author Daniel Liesner (8983001)
Lisa N. S. Shama (6102515)
Nora Diehl (8983004)
Klaus Valentin (139492)
Inka Bartsch (6942989)
author_facet Daniel Liesner (8983001)
Lisa N. S. Shama (6102515)
Nora Diehl (8983004)
Klaus Valentin (139492)
Inka Bartsch (6942989)
author_sort Daniel Liesner (8983001)
title Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx
title_short Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx
title_full Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx
title_fullStr Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx
title_full_unstemmed Data_Sheet_1_Thermal Plasticity of the Kelp Laminaria digitata (Phaeophyceae) Across Life Cycle Stages Reveals the Importance of Cold Seasons for Marine Forests.docx
title_sort data_sheet_1_thermal plasticity of the kelp laminaria digitata (phaeophyceae) across life cycle stages reveals the importance of cold seasons for marine forests.docx
publishDate 2020
url https://doi.org/10.3389/fmars.2020.00456.s001
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_relation https://figshare.com/articles/Data_Sheet_1_Thermal_Plasticity_of_the_Kelp_Laminaria_digitata_Phaeophyceae_Across_Life_Cycle_Stages_Reveals_the_Importance_of_Cold_Seasons_for_Marine_Forests_docx/12495749
doi:10.3389/fmars.2020.00456.s001
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fmars.2020.00456.s001
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