Dataset: Contrasting exposure effects on copepod respiration
Includes main data "Respiration and predation.csv" and blank measurements "normBlanks_respiration and predation.csv". We exposed two species of copepods (Tigriopus brevicornis and Calanus finmarchicus) to the treatments predation risk, copper, combined predation risk and copper,...
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Online Access: | https://doi.org/10.6084/m9.figshare.12326219.v1 |
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ftpurdueuniv:oai:figshare.com:article/12326219 2023-05-15T15:47:59+02:00 Dataset: Contrasting exposure effects on copepod respiration Torben Lode (8859554) Jan Heuschele (7329251) Tom Andersen (7329309) Josefin Titelman (7329307) Ketil Hylland (8859555) Katrine Borgå (8494332) 2020-06-15T12:15:44Z https://doi.org/10.6084/m9.figshare.12326219.v1 unknown https://figshare.com/articles/Dataset_Contrasting_exposure_effects_on_copepod_respiration/12326219 doi:10.6084/m9.figshare.12326219.v1 CC BY 4.0 CC-BY Marine Biology Ecotoxicology Copepod Respiration Copper Predation risk Kairomone Multiple stressors Dataset 2020 ftpurdueuniv https://doi.org/10.6084/m9.figshare.12326219.v1 2020-06-25T10:43:23Z Includes main data "Respiration and predation.csv" and blank measurements "normBlanks_respiration and predation.csv". We exposed two species of copepods (Tigriopus brevicornis and Calanus finmarchicus) to the treatments predation risk, copper, combined predation risk and copper, or control. We measured incubation chamber oxygen levels continuously throughout a 12 hour exposure period and calculated the median per dry weight respiration rates per replicate. Thus, only one time-point is included (i.e. TimeHours=1.125), but medians are extracted for all relevant exposure intervals for investigation. Dataset column explanations UniqueID - a per replicate unique ID based on well plate positioning, treatment and series. Well.ID - replicate position on 24 well cell culture plates used for incubation. TimeHours - time (hours) into exposure period. We removed the first hour due to acclimation. Series - we conducted four experimental rounds which we refer to as series. SDR - refer to Sensor Dish Reader (SDR) units used for measuring oxygen. We used four SDR-units per series. Treatment - we exposed copepods to predation risk, copper, combined predation risk and copper, or control. RespRate_corr.nlm_0to12h - extracted median of influx-corrected negated oxygen difference (i.e. oxygen consumption rates) per replicate well for the full exposure period 0-12 h. Medians were similarly extracted for the time-intervals 0-3 h, 3-6 h, 6-9 h and 9-12 h. Oxygen consumption rates are given as µmol O2 per replicate well per h. Well - replicate position on 24 well cell culture plate used for incubation Predation risk - three-spined stickleback chemical cue exposure, 0 = no, 1 = yes. Cu - copper exposure, 0 = no, 1 = yes. Species - copepod, Tigriopus brevicornis, Calanus finmarchicus male - number of individuals. female - number of individuals. juveniles - number of individuals. DmgAntenna - registered external physical injuries post exposure. WhiteDot - registered external physical abnormalities post exposure. Alive - number of surviving individuals post exposure. Dead - number of dead individuals post exposure. BigBubble - registered potentially confounding gas bubble. length.um - copepod prosome length, µm. DWeight - ash-free dry weight, calculated from prosome length. DWResp_0to12h - equals RespRate_corr.nlm_0to12h normalized by biomass to µmol O2 per µg.dw per h. Similarly calculated for the time-intervals 0-3 h, 3-6 h, 6-9 h and 9-12 h. Dataset Calanus finmarchicus Copepods Purdue University: e-Pubs |
institution |
Open Polar |
collection |
Purdue University: e-Pubs |
op_collection_id |
ftpurdueuniv |
language |
unknown |
topic |
Marine Biology Ecotoxicology Copepod Respiration Copper Predation risk Kairomone Multiple stressors |
spellingShingle |
Marine Biology Ecotoxicology Copepod Respiration Copper Predation risk Kairomone Multiple stressors Torben Lode (8859554) Jan Heuschele (7329251) Tom Andersen (7329309) Josefin Titelman (7329307) Ketil Hylland (8859555) Katrine Borgå (8494332) Dataset: Contrasting exposure effects on copepod respiration |
topic_facet |
Marine Biology Ecotoxicology Copepod Respiration Copper Predation risk Kairomone Multiple stressors |
description |
Includes main data "Respiration and predation.csv" and blank measurements "normBlanks_respiration and predation.csv". We exposed two species of copepods (Tigriopus brevicornis and Calanus finmarchicus) to the treatments predation risk, copper, combined predation risk and copper, or control. We measured incubation chamber oxygen levels continuously throughout a 12 hour exposure period and calculated the median per dry weight respiration rates per replicate. Thus, only one time-point is included (i.e. TimeHours=1.125), but medians are extracted for all relevant exposure intervals for investigation. Dataset column explanations UniqueID - a per replicate unique ID based on well plate positioning, treatment and series. Well.ID - replicate position on 24 well cell culture plates used for incubation. TimeHours - time (hours) into exposure period. We removed the first hour due to acclimation. Series - we conducted four experimental rounds which we refer to as series. SDR - refer to Sensor Dish Reader (SDR) units used for measuring oxygen. We used four SDR-units per series. Treatment - we exposed copepods to predation risk, copper, combined predation risk and copper, or control. RespRate_corr.nlm_0to12h - extracted median of influx-corrected negated oxygen difference (i.e. oxygen consumption rates) per replicate well for the full exposure period 0-12 h. Medians were similarly extracted for the time-intervals 0-3 h, 3-6 h, 6-9 h and 9-12 h. Oxygen consumption rates are given as µmol O2 per replicate well per h. Well - replicate position on 24 well cell culture plate used for incubation Predation risk - three-spined stickleback chemical cue exposure, 0 = no, 1 = yes. Cu - copper exposure, 0 = no, 1 = yes. Species - copepod, Tigriopus brevicornis, Calanus finmarchicus male - number of individuals. female - number of individuals. juveniles - number of individuals. DmgAntenna - registered external physical injuries post exposure. WhiteDot - registered external physical abnormalities post exposure. Alive - number of surviving individuals post exposure. Dead - number of dead individuals post exposure. BigBubble - registered potentially confounding gas bubble. length.um - copepod prosome length, µm. DWeight - ash-free dry weight, calculated from prosome length. DWResp_0to12h - equals RespRate_corr.nlm_0to12h normalized by biomass to µmol O2 per µg.dw per h. Similarly calculated for the time-intervals 0-3 h, 3-6 h, 6-9 h and 9-12 h. |
format |
Dataset |
author |
Torben Lode (8859554) Jan Heuschele (7329251) Tom Andersen (7329309) Josefin Titelman (7329307) Ketil Hylland (8859555) Katrine Borgå (8494332) |
author_facet |
Torben Lode (8859554) Jan Heuschele (7329251) Tom Andersen (7329309) Josefin Titelman (7329307) Ketil Hylland (8859555) Katrine Borgå (8494332) |
author_sort |
Torben Lode (8859554) |
title |
Dataset: Contrasting exposure effects on copepod respiration |
title_short |
Dataset: Contrasting exposure effects on copepod respiration |
title_full |
Dataset: Contrasting exposure effects on copepod respiration |
title_fullStr |
Dataset: Contrasting exposure effects on copepod respiration |
title_full_unstemmed |
Dataset: Contrasting exposure effects on copepod respiration |
title_sort |
dataset: contrasting exposure effects on copepod respiration |
publishDate |
2020 |
url |
https://doi.org/10.6084/m9.figshare.12326219.v1 |
genre |
Calanus finmarchicus Copepods |
genre_facet |
Calanus finmarchicus Copepods |
op_relation |
https://figshare.com/articles/Dataset_Contrasting_exposure_effects_on_copepod_respiration/12326219 doi:10.6084/m9.figshare.12326219.v1 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.12326219.v1 |
_version_ |
1766382975394512896 |