A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins
We report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to select optimal candidates for a recombinant subunit vaccine to protect fish fro...
Published in: | Vaccine |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
ELSEVIER SCI LTD
2024
|
Subjects: | |
Online Access: | https://repositorio.uc.cl/handle/11534/75792 https://doi.org/10.1016/j.vaccine.2006.03.027 |
id |
ftpunivcchile:oai:repositorio.uc.cl:11534/75792 |
---|---|
record_format |
openpolar |
spelling |
ftpunivcchile:oai:repositorio.uc.cl:11534/75792 2024-05-12T08:01:21+00:00 A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins Wilhelm, Vivian Miquel, Alvaro Burzio, Luis O. Rosemblatt, Mario Engel, Esteban Valenzuela, Sebastián Parada, Gustavo Valenzuela, Pablo D. T. 2024-01-10T12:04:25Z 9 páginas application/pdf https://repositorio.uc.cl/handle/11534/75792 https://doi.org/10.1016/j.vaccine.2006.03.027 en eng ELSEVIER SCI LTD doi:10.1016/j.vaccine.2006.03.027 1873-2518 0264-410X MEDLINE:16697089 https://doi.org/10.1016/j.vaccine.2006.03.027 https://repositorio.uc.cl/handle/11534/75792 WOS:000238557500023 registro bibliográfico Piscirickettsia salmonis recombinant proteins subunit vaccine recombinant antigens LEGIONELLA-PNEUMOPHILA MONOCLONAL-ANTIBODIES NUCLEOTIDE-SEQUENCE ESCHERICHIA-COLI MEMBRANE-PROTEIN ATLANTIC SALMON FISH GENE EXPRESSION VIRULENCE 03 Good Health and Well-being 03 Salud y bienestar artículo 2024 ftpunivcchile https://doi.org/10.1016/j.vaccine.2006.03.027 2024-04-17T14:44:59Z We report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to select optimal candidates for a recombinant subunit vaccine to protect fish from rickettsial septicaemia (SRS). Based on this information, 15 P. salmonis ORFs encoding heat shock proteins, virulence factors, membrane bound and other surface exposed antigens, were isolated and expressed. Seven of the most promising antigens were formulated in three mixtures (V1-V3) containing two or three recombinant proteins each and injected into salmon to test their protective efficacy. Two of the three formulations (V1, V2) elicited a strong protective response in a challenge against the pathogen, which was coincident with the humoral response against the corresponding recombinant proteins present in each formulation. V I, formulated with recombinant chaperonines Hsp60, Hsp70 and flagellar protein FlgG of P. salmonis achieved the highest level of protection with a relative percent survival (RPS) of 95%. (c) 2006 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Atlantic salmon Pontificia Universidad Católica de Chile: Repositorio UC Vaccine 24 23 5083 5091 |
institution |
Open Polar |
collection |
Pontificia Universidad Católica de Chile: Repositorio UC |
op_collection_id |
ftpunivcchile |
language |
English |
topic |
Piscirickettsia salmonis recombinant proteins subunit vaccine recombinant antigens LEGIONELLA-PNEUMOPHILA MONOCLONAL-ANTIBODIES NUCLEOTIDE-SEQUENCE ESCHERICHIA-COLI MEMBRANE-PROTEIN ATLANTIC SALMON FISH GENE EXPRESSION VIRULENCE 03 Good Health and Well-being 03 Salud y bienestar |
spellingShingle |
Piscirickettsia salmonis recombinant proteins subunit vaccine recombinant antigens LEGIONELLA-PNEUMOPHILA MONOCLONAL-ANTIBODIES NUCLEOTIDE-SEQUENCE ESCHERICHIA-COLI MEMBRANE-PROTEIN ATLANTIC SALMON FISH GENE EXPRESSION VIRULENCE 03 Good Health and Well-being 03 Salud y bienestar Wilhelm, Vivian Miquel, Alvaro Burzio, Luis O. Rosemblatt, Mario Engel, Esteban Valenzuela, Sebastián Parada, Gustavo Valenzuela, Pablo D. T. A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins |
topic_facet |
Piscirickettsia salmonis recombinant proteins subunit vaccine recombinant antigens LEGIONELLA-PNEUMOPHILA MONOCLONAL-ANTIBODIES NUCLEOTIDE-SEQUENCE ESCHERICHIA-COLI MEMBRANE-PROTEIN ATLANTIC SALMON FISH GENE EXPRESSION VIRULENCE 03 Good Health and Well-being 03 Salud y bienestar |
description |
We report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to select optimal candidates for a recombinant subunit vaccine to protect fish from rickettsial septicaemia (SRS). Based on this information, 15 P. salmonis ORFs encoding heat shock proteins, virulence factors, membrane bound and other surface exposed antigens, were isolated and expressed. Seven of the most promising antigens were formulated in three mixtures (V1-V3) containing two or three recombinant proteins each and injected into salmon to test their protective efficacy. Two of the three formulations (V1, V2) elicited a strong protective response in a challenge against the pathogen, which was coincident with the humoral response against the corresponding recombinant proteins present in each formulation. V I, formulated with recombinant chaperonines Hsp60, Hsp70 and flagellar protein FlgG of P. salmonis achieved the highest level of protection with a relative percent survival (RPS) of 95%. (c) 2006 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Wilhelm, Vivian Miquel, Alvaro Burzio, Luis O. Rosemblatt, Mario Engel, Esteban Valenzuela, Sebastián Parada, Gustavo Valenzuela, Pablo D. T. |
author_facet |
Wilhelm, Vivian Miquel, Alvaro Burzio, Luis O. Rosemblatt, Mario Engel, Esteban Valenzuela, Sebastián Parada, Gustavo Valenzuela, Pablo D. T. |
author_sort |
Wilhelm, Vivian |
title |
A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins |
title_short |
A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins |
title_full |
A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins |
title_fullStr |
A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins |
title_full_unstemmed |
A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins |
title_sort |
vaccine against the salmonid pathogen piscirickettsia salmonis based on recombinant proteins |
publisher |
ELSEVIER SCI LTD |
publishDate |
2024 |
url |
https://repositorio.uc.cl/handle/11534/75792 https://doi.org/10.1016/j.vaccine.2006.03.027 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
doi:10.1016/j.vaccine.2006.03.027 1873-2518 0264-410X MEDLINE:16697089 https://doi.org/10.1016/j.vaccine.2006.03.027 https://repositorio.uc.cl/handle/11534/75792 WOS:000238557500023 |
op_rights |
registro bibliográfico |
op_doi |
https://doi.org/10.1016/j.vaccine.2006.03.027 |
container_title |
Vaccine |
container_volume |
24 |
container_issue |
23 |
container_start_page |
5083 |
op_container_end_page |
5091 |
_version_ |
1798843475409502208 |