Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans
The production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature...
Published in: | American Journal of Biochemistry and Biotechnology |
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Online Access: | https://repositorio.puce.edu.ec/handle/123456789/4472 https://doi.org/10.3844/ajbbsp.2019.75.82 https://thescipub.com/pdf/ajbbsp.2019.75.82.pdf |
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ftpucecuador:oai:repositorio.puce.edu.ec:123456789/4472 2024-09-15T17:46:35+00:00 Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans Carvajal Barriga, Enrique Javier Portero Barahona, Patricia Carvajal Barriga, Enrique Javier 2019 https://repositorio.puce.edu.ec/handle/123456789/4472 https://doi.org/10.3844/ajbbsp.2019.75.82 https://thescipub.com/pdf/ajbbsp.2019.75.82.pdf unknown 1553-3468 1558-6332 https://doi.org/10.3844/ajbbsp.2019.75.82 https://thescipub.com/pdf/ajbbsp.2019.75.82.pdf https://repositorio.puce.edu.ec/handle/123456789/4472 OpenAccess Proteínas Lipasa Levadura 2019 ftpucecuador https://doi.org/10.3844/ajbbsp.2019.75.82 2024-06-24T03:27:37Z The production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase is determined. Lipase production was 0.27 U/mL, a high value compared to lipolytic activities in non-optimized media. However, this value can be increased by optimizing the culture medium. The lipase of G. pullulans has maximum activity at pH 8.0 and 40°C (thermal stability 40-50°C). Regarding the kinetic parameters, a KM = 3.7×10-4 M was obtained, a value located in the range of industrial lipases. In addition, its kinetics presented the phenomenon of interfacial activation. The results presented in this work show the biotechnological potential of the lipase due its biochemical properties and are useful for later work directed to study other factors that affect the enzyme activity and potential biotechnological applications of the Guehomyces pullulans lipase. Other/Unknown Material Antarc* Antarctic Pontificia Universidad Catolica del Ecuador: Repositorio Digital PUCE American Journal of Biochemistry and Biotechnology 15 2 75 82 |
institution |
Open Polar |
collection |
Pontificia Universidad Catolica del Ecuador: Repositorio Digital PUCE |
op_collection_id |
ftpucecuador |
language |
unknown |
topic |
Proteínas Lipasa Levadura |
spellingShingle |
Proteínas Lipasa Levadura Carvajal Barriga, Enrique Javier Portero Barahona, Patricia Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans |
topic_facet |
Proteínas Lipasa Levadura |
description |
The production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase is determined. Lipase production was 0.27 U/mL, a high value compared to lipolytic activities in non-optimized media. However, this value can be increased by optimizing the culture medium. The lipase of G. pullulans has maximum activity at pH 8.0 and 40°C (thermal stability 40-50°C). Regarding the kinetic parameters, a KM = 3.7×10-4 M was obtained, a value located in the range of industrial lipases. In addition, its kinetics presented the phenomenon of interfacial activation. The results presented in this work show the biotechnological potential of the lipase due its biochemical properties and are useful for later work directed to study other factors that affect the enzyme activity and potential biotechnological applications of the Guehomyces pullulans lipase. |
author2 |
Carvajal Barriga, Enrique Javier |
author |
Carvajal Barriga, Enrique Javier Portero Barahona, Patricia |
author_facet |
Carvajal Barriga, Enrique Javier Portero Barahona, Patricia |
author_sort |
Carvajal Barriga, Enrique Javier |
title |
Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans |
title_short |
Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans |
title_full |
Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans |
title_fullStr |
Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans |
title_full_unstemmed |
Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans |
title_sort |
production, extraction and characterization of lipases from the antarctic yeast guehomyces pullulans |
publishDate |
2019 |
url |
https://repositorio.puce.edu.ec/handle/123456789/4472 https://doi.org/10.3844/ajbbsp.2019.75.82 https://thescipub.com/pdf/ajbbsp.2019.75.82.pdf |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
1553-3468 1558-6332 https://doi.org/10.3844/ajbbsp.2019.75.82 https://thescipub.com/pdf/ajbbsp.2019.75.82.pdf https://repositorio.puce.edu.ec/handle/123456789/4472 |
op_rights |
OpenAccess |
op_doi |
https://doi.org/10.3844/ajbbsp.2019.75.82 |
container_title |
American Journal of Biochemistry and Biotechnology |
container_volume |
15 |
container_issue |
2 |
container_start_page |
75 |
op_container_end_page |
82 |
_version_ |
1810494845975789568 |