Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and...
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2022
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Online Access: | https://repositorio.uca.edu.ar/handle/123456789/16424 https://doi.org/10.3390/bioengineering9110694 |
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ftpucargentir:oai:ucacris:123456789/16424 2024-04-28T08:00:10+00:00 Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley 2022 application/pdf https://repositorio.uca.edu.ar/handle/123456789/16424 https://doi.org/10.3390/bioengineering9110694 eng eng MDPI AG Machado, S. et al. Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor [en línea]. Bioengineering. 2022, 9 (11). doi:10.3390/bioengineering9110694. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16424 2306-5354 (online) https://repositorio.uca.edu.ar/handle/123456789/16424 doi:10.3390/bioengineering9110694 Acceso abierto Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ Bioengineering. Vol.9, No.11, 2022 EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO Artículo 2022 ftpucargentir https://doi.org/10.3390/bioengineering9110694 2024-04-02T16:48:02Z Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment. Article in Journal/Newspaper Antarc* Antarctic Repositorio Institucional UCA (Pontificia Universidad Católica Argentina) Bioengineering 9 11 694 |
institution |
Open Polar |
collection |
Repositorio Institucional UCA (Pontificia Universidad Católica Argentina) |
op_collection_id |
ftpucargentir |
language |
English |
topic |
EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO |
spellingShingle |
EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
topic_facet |
EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO |
description |
Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment. |
format |
Article in Journal/Newspaper |
author |
Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley |
author_facet |
Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley |
author_sort |
Machado, Suellen |
title |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_short |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_full |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_fullStr |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_full_unstemmed |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_sort |
effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://repositorio.uca.edu.ar/handle/123456789/16424 https://doi.org/10.3390/bioengineering9110694 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Bioengineering. Vol.9, No.11, 2022 |
op_relation |
Machado, S. et al. Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor [en línea]. Bioengineering. 2022, 9 (11). doi:10.3390/bioengineering9110694. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16424 2306-5354 (online) https://repositorio.uca.edu.ar/handle/123456789/16424 doi:10.3390/bioengineering9110694 |
op_rights |
Acceso abierto Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ |
op_doi |
https://doi.org/10.3390/bioengineering9110694 |
container_title |
Bioengineering |
container_volume |
9 |
container_issue |
11 |
container_start_page |
694 |
_version_ |
1797572525024083968 |