Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis, two predominant zoonotic helminths circulating...
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ftpubmed:oai:www.ncbi.nlm.nih.gov/pmc:4959075 2023-05-15T15:49:51+02:00 Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence 2016-05-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959075/ http://www.ncbi.nlm.nih.gov/pubmed/26969697 https://doi.org/10.1128/AEM.03467-15 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959075/ http://www.ncbi.nlm.nih.gov/pubmed/26969697 http://dx.doi.org/10.1128/AEM.03467-15 Copyright © 2016, American Society for Microbiology. All Rights Reserved. Genetics and Molecular Biology Text 2016 ftpubmed https://doi.org/10.1128/AEM.03467-15 2016-11-06T01:18:23Z Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis, two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes, Canis lupus familiaris, Canis lupus, Felis silvestris catus, Meles meles, Martes foina, and Martes martes. With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed (E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens. Text Canis lupus PubMed Central (PMC) Applied and Environmental Microbiology 82 10 2950 2958 |
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Genetics and Molecular Biology Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
topic_facet |
Genetics and Molecular Biology |
description |
Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis, two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes, Canis lupus familiaris, Canis lupus, Felis silvestris catus, Meles meles, Martes foina, and Martes martes. With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed (E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens. |
format |
Text |
author |
Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence |
author_facet |
Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence |
author_sort |
Knapp, Jenny |
title |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_short |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_full |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_fullStr |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_full_unstemmed |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_sort |
development of a real-time pcr for a sensitive one-step coprodiagnosis allowing both the identification of carnivore feces and the detection of toxocara spp. and echinococcus multilocularis |
publisher |
American Society for Microbiology |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959075/ http://www.ncbi.nlm.nih.gov/pubmed/26969697 https://doi.org/10.1128/AEM.03467-15 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959075/ http://www.ncbi.nlm.nih.gov/pubmed/26969697 http://dx.doi.org/10.1128/AEM.03467-15 |
op_rights |
Copyright © 2016, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.03467-15 |
container_title |
Applied and Environmental Microbiology |
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82 |
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10 |
container_start_page |
2950 |
op_container_end_page |
2958 |
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1766384863397543936 |