Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine tw...
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ftpubmed:oai:pubmedcentral.nih.gov:9986475 2023-05-15T17:44:50+02:00 Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber 2023-02-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986475/ http://www.ncbi.nlm.nih.gov/pubmed/36890907 https://doi.org/10.3389/fpls.2023.1111001 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986475/ http://www.ncbi.nlm.nih.gov/pubmed/36890907 http://dx.doi.org/10.3389/fpls.2023.1111001 Copyright © 2023 Siddique, Menke, Dinedurga and Albrectsen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Front Plant Sci Plant Science Text 2023 ftpubmed https://doi.org/10.3389/fpls.2023.1111001 2023-03-12T01:59:50Z Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine twisting rust) is of concern in Swedish forestry, and here we evaluate the use of visible rust scores (VRS) on its obligate summer host, European aspen (Populus tremula) as a tool for quantification of the pathogen. With use of species-specific primers, we could detect the native rust, but we failed to detect two exotic rusts (M. medusae and M. larici-populina). We found that aspen genotype determined the presence of fungal genetic markers (amplifying the ITS2 region of the fungal rDNA sequence) as well as DNA sequences specific to M. pinitorqua. We correlated VRS with the amount of fungal DNA in the same leaf, and we related the findings to aspen genotype-specific parameters such as the ability to synthesize and store leaf condensed tannins (CT). At the genotype level both positive and negative relationships were observed between CTs, fungal markers, and rust infestations. However, at the population level, foliar CT concentrations correlated negatively with general fungal- and rust-specific marker abundances. Our results, therefore, do not support the use of VRS to assess Melampsora infestation in Aspen. They do, however, suggest that the relationship between European aspen and rust infestation may be characterized as autochthonous in northern Sweden. Text Northern Sweden PubMed Central (PMC) Frontiers in Plant Science 14 |
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Plant Science Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
topic_facet |
Plant Science |
description |
Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine twisting rust) is of concern in Swedish forestry, and here we evaluate the use of visible rust scores (VRS) on its obligate summer host, European aspen (Populus tremula) as a tool for quantification of the pathogen. With use of species-specific primers, we could detect the native rust, but we failed to detect two exotic rusts (M. medusae and M. larici-populina). We found that aspen genotype determined the presence of fungal genetic markers (amplifying the ITS2 region of the fungal rDNA sequence) as well as DNA sequences specific to M. pinitorqua. We correlated VRS with the amount of fungal DNA in the same leaf, and we related the findings to aspen genotype-specific parameters such as the ability to synthesize and store leaf condensed tannins (CT). At the genotype level both positive and negative relationships were observed between CTs, fungal markers, and rust infestations. However, at the population level, foliar CT concentrations correlated negatively with general fungal- and rust-specific marker abundances. Our results, therefore, do not support the use of VRS to assess Melampsora infestation in Aspen. They do, however, suggest that the relationship between European aspen and rust infestation may be characterized as autochthonous in northern Sweden. |
format |
Text |
author |
Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber |
author_facet |
Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber |
author_sort |
Siddique, Abu Bakar |
title |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_short |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_full |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_fullStr |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_full_unstemmed |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_sort |
molecular studies of rust on european aspen suggest an autochthonous relationship shaped by genotype |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986475/ http://www.ncbi.nlm.nih.gov/pubmed/36890907 https://doi.org/10.3389/fpls.2023.1111001 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Front Plant Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986475/ http://www.ncbi.nlm.nih.gov/pubmed/36890907 http://dx.doi.org/10.3389/fpls.2023.1111001 |
op_rights |
Copyright © 2023 Siddique, Menke, Dinedurga and Albrectsen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_doi |
https://doi.org/10.3389/fpls.2023.1111001 |
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Frontiers in Plant Science |
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14 |
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1766147129100730368 |