Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments
Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the δ subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophil...
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ftpubmed:oai:pubmedcentral.nih.gov:99729 2023-05-15T14:55:16+02:00 Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments Sahm, Kerstin Knoblauch, Christian Amann, Rudolf 1999-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99729 http://www.ncbi.nlm.nih.gov/pubmed/10473404 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99729 http://www.ncbi.nlm.nih.gov/pubmed/10473404 Copyright © 1999, American Society for Microbiology General Microbial Ecology Text 1999 ftpubmed 2013-08-29T09:45:58Z Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the δ subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes, was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3.5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments. Text Arctic Hornsund Svalbard Spitsbergen PubMed Central (PMC) Arctic Arctic Island ENVELOPE(-74.766,-74.766,62.234,62.234) Hornsund ENVELOPE(15.865,15.865,76.979,76.979) Svalbard |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
General Microbial Ecology |
spellingShingle |
General Microbial Ecology Sahm, Kerstin Knoblauch, Christian Amann, Rudolf Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
topic_facet |
General Microbial Ecology |
description |
Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the δ subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes, was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3.5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments. |
format |
Text |
author |
Sahm, Kerstin Knoblauch, Christian Amann, Rudolf |
author_facet |
Sahm, Kerstin Knoblauch, Christian Amann, Rudolf |
author_sort |
Sahm, Kerstin |
title |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_short |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_full |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_fullStr |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_full_unstemmed |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_sort |
phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine arctic sediments |
publisher |
American Society for Microbiology |
publishDate |
1999 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99729 http://www.ncbi.nlm.nih.gov/pubmed/10473404 |
long_lat |
ENVELOPE(-74.766,-74.766,62.234,62.234) ENVELOPE(15.865,15.865,76.979,76.979) |
geographic |
Arctic Arctic Island Hornsund Svalbard |
geographic_facet |
Arctic Arctic Island Hornsund Svalbard |
genre |
Arctic Hornsund Svalbard Spitsbergen |
genre_facet |
Arctic Hornsund Svalbard Spitsbergen |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99729 http://www.ncbi.nlm.nih.gov/pubmed/10473404 |
op_rights |
Copyright © 1999, American Society for Microbiology |
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