Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers
Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer...
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2023
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9969050/ http://www.ncbi.nlm.nih.gov/pubmed/36861025 https://doi.org/10.1002/ece3.9785 |
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ftpubmed:oai:pubmedcentral.nih.gov:9969050 2023-05-15T13:27:11+02:00 Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun 2023-02-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9969050/ http://www.ncbi.nlm.nih.gov/pubmed/36861025 https://doi.org/10.1002/ece3.9785 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9969050/ http://www.ncbi.nlm.nih.gov/pubmed/36861025 http://dx.doi.org/10.1002/ece3.9785 © 2023 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Ecol Evol Research Articles Text 2023 ftpubmed https://doi.org/10.1002/ece3.9785 2023-03-05T02:28:16Z Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel (Anguilla anguilla) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification. Text Anguilla anguilla European eel PubMed Central (PMC) Ecology and Evolution 13 2 |
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Research Articles |
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Research Articles Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
topic_facet |
Research Articles |
description |
Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel (Anguilla anguilla) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification. |
format |
Text |
author |
Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun |
author_facet |
Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun |
author_sort |
Halvorsen, Silje |
title |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_short |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_full |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_fullStr |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_full_unstemmed |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_sort |
estimating number of european eel (anguilla anguilla) individuals using environmental dna and haplotype count in small rivers |
publisher |
John Wiley and Sons Inc. |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9969050/ http://www.ncbi.nlm.nih.gov/pubmed/36861025 https://doi.org/10.1002/ece3.9785 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Ecol Evol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9969050/ http://www.ncbi.nlm.nih.gov/pubmed/36861025 http://dx.doi.org/10.1002/ece3.9785 |
op_rights |
© 2023 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.9785 |
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Ecology and Evolution |
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13 |
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2 |
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1766396883571310592 |