Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia)
Being diverse and widely distributed globally, bats are a known reservoir of a series of emerging zoonotic viruses. We studied fecal viromes of twenty-six bats captured in 2015 in the Moscow Region and found 13 of 26 (50%) samples to be coronavirus positive. Of P. nathusii (the Nathusius’ pipistrell...
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ftpubmed:oai:pubmedcentral.nih.gov:9965006 2023-05-15T17:59:45+02:00 Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia) Speranskaya, Anna S. Artiushin, Ilia V. Samoilov, Andrei E. Korneenko, Elena V. Khabudaev, Kirill V. Ilina, Elena N. Yusefovich, Alexander P. Safonova, Marina V. Dolgova, Anna S. Gladkikh, Anna S. Dedkov, Vladimir G. Daszak, Peter 2023-02-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965006/ http://www.ncbi.nlm.nih.gov/pubmed/36834395 https://doi.org/10.3390/ijerph20043702 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965006/ http://www.ncbi.nlm.nih.gov/pubmed/36834395 http://dx.doi.org/10.3390/ijerph20043702 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Int J Environ Res Public Health Article Text 2023 ftpubmed https://doi.org/10.3390/ijerph20043702 2023-03-05T02:15:21Z Being diverse and widely distributed globally, bats are a known reservoir of a series of emerging zoonotic viruses. We studied fecal viromes of twenty-six bats captured in 2015 in the Moscow Region and found 13 of 26 (50%) samples to be coronavirus positive. Of P. nathusii (the Nathusius’ pipistrelle), 3 of 6 samples were carriers of a novel MERS-related betacoronavirus. We sequenced and assembled the complete genome of this betacoronavirus and named it MOW-BatCoV strain 15-22. Whole genome phylogenetic analysis suggests that MOW-BatCoV/15-22 falls into a distinct subclade closely related to human and camel MERS-CoV. Unexpectedly, the phylogenetic analysis of the novel MOW-BatCoV/15-22 spike gene showed the closest similarity to CoVs from Erinaceus europaeus (European hedgehog). We suppose MOW-BatCoV could have arisen as a result of recombination between ancestral viruses of bats and hedgehogs. Molecular docking analysis of MOW-BatCoV/15-22 spike glycoprotein binding to DPP4 receptors of different mammals predicted the highest binding ability with DPP4 of the Myotis brandtii bat (docking score −320.15) and the E. europaeus (docking score –294.51). Hedgehogs are widely kept as pets and are commonly found in areas of human habitation. As this novel bat-CoV is likely capable of infecting hedgehogs, we suggest hedgehogs can act as intermediate hosts between bats and humans for other bat-CoVs. Text Pipistrellus nathusii PubMed Central (PMC) International Journal of Environmental Research and Public Health 20 4 3702 |
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Article Speranskaya, Anna S. Artiushin, Ilia V. Samoilov, Andrei E. Korneenko, Elena V. Khabudaev, Kirill V. Ilina, Elena N. Yusefovich, Alexander P. Safonova, Marina V. Dolgova, Anna S. Gladkikh, Anna S. Dedkov, Vladimir G. Daszak, Peter Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia) |
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description |
Being diverse and widely distributed globally, bats are a known reservoir of a series of emerging zoonotic viruses. We studied fecal viromes of twenty-six bats captured in 2015 in the Moscow Region and found 13 of 26 (50%) samples to be coronavirus positive. Of P. nathusii (the Nathusius’ pipistrelle), 3 of 6 samples were carriers of a novel MERS-related betacoronavirus. We sequenced and assembled the complete genome of this betacoronavirus and named it MOW-BatCoV strain 15-22. Whole genome phylogenetic analysis suggests that MOW-BatCoV/15-22 falls into a distinct subclade closely related to human and camel MERS-CoV. Unexpectedly, the phylogenetic analysis of the novel MOW-BatCoV/15-22 spike gene showed the closest similarity to CoVs from Erinaceus europaeus (European hedgehog). We suppose MOW-BatCoV could have arisen as a result of recombination between ancestral viruses of bats and hedgehogs. Molecular docking analysis of MOW-BatCoV/15-22 spike glycoprotein binding to DPP4 receptors of different mammals predicted the highest binding ability with DPP4 of the Myotis brandtii bat (docking score −320.15) and the E. europaeus (docking score –294.51). Hedgehogs are widely kept as pets and are commonly found in areas of human habitation. As this novel bat-CoV is likely capable of infecting hedgehogs, we suggest hedgehogs can act as intermediate hosts between bats and humans for other bat-CoVs. |
format |
Text |
author |
Speranskaya, Anna S. Artiushin, Ilia V. Samoilov, Andrei E. Korneenko, Elena V. Khabudaev, Kirill V. Ilina, Elena N. Yusefovich, Alexander P. Safonova, Marina V. Dolgova, Anna S. Gladkikh, Anna S. Dedkov, Vladimir G. Daszak, Peter |
author_facet |
Speranskaya, Anna S. Artiushin, Ilia V. Samoilov, Andrei E. Korneenko, Elena V. Khabudaev, Kirill V. Ilina, Elena N. Yusefovich, Alexander P. Safonova, Marina V. Dolgova, Anna S. Gladkikh, Anna S. Dedkov, Vladimir G. Daszak, Peter |
author_sort |
Speranskaya, Anna S. |
title |
Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia) |
title_short |
Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia) |
title_full |
Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia) |
title_fullStr |
Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia) |
title_full_unstemmed |
Identification and Genetic Characterization of MERS-Related Coronavirus Isolated from Nathusius’ Pipistrelle (Pipistrellus nathusii) near Zvenigorod (Moscow Region, Russia) |
title_sort |
identification and genetic characterization of mers-related coronavirus isolated from nathusius’ pipistrelle (pipistrellus nathusii) near zvenigorod (moscow region, russia) |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965006/ http://www.ncbi.nlm.nih.gov/pubmed/36834395 https://doi.org/10.3390/ijerph20043702 |
genre |
Pipistrellus nathusii |
genre_facet |
Pipistrellus nathusii |
op_source |
Int J Environ Res Public Health |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965006/ http://www.ncbi.nlm.nih.gov/pubmed/36834395 http://dx.doi.org/10.3390/ijerph20043702 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ijerph20043702 |
container_title |
International Journal of Environmental Research and Public Health |
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20 |
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4 |
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3702 |
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1766168637344841728 |