Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus
Staphylococcus aureus is a Gram-positive opportunistic human pathogen responsible for severe infections and thousands of deaths annually, mostly due to its multidrug-resistant (MDR) variants. The cell membrane has emerged as a promising new therapeutic target, and lipophilic molecules, such as biosu...
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ftpubmed:oai:pubmedcentral.nih.gov:9958974 2023-05-15T13:40:39+02:00 Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus Buonocore, Carmine Giugliano, Rosa Della Sala, Gerardo Palma Esposito, Fortunato Tedesco, Pietro Folliero, Veronica Galdiero, Massimiliano Franci, Gianluigi de Pascale, Donatella 2023-02-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958974/ http://www.ncbi.nlm.nih.gov/pubmed/36840022 https://doi.org/10.3390/pharmaceutics15020700 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958974/ http://www.ncbi.nlm.nih.gov/pubmed/36840022 http://dx.doi.org/10.3390/pharmaceutics15020700 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Pharmaceutics Article Text 2023 ftpubmed https://doi.org/10.3390/pharmaceutics15020700 2023-03-05T01:54:20Z Staphylococcus aureus is a Gram-positive opportunistic human pathogen responsible for severe infections and thousands of deaths annually, mostly due to its multidrug-resistant (MDR) variants. The cell membrane has emerged as a promising new therapeutic target, and lipophilic molecules, such as biosurfactants, are currently being utilized. Herein, we evaluated the antimicrobial activity of a rhamnolipids mixture produced by the Antarctic marine bacterium Pseudomonas gessardii M15. We demonstrated that our mixture has bactericidal activity in the range of 12.5–50 µg/mL against a panel of clinical MDR isolates of S. aureus, and that the mixture eradicated the bacterial population in 30 min at MIC value, and in 5 min after doubling the concentration. We also tested abilities of RLs to interfere with biofilm at different stages and determined that RLs can penetrate biofilm and kill the bacteria at sub-MICs values. The mixture was then used to functionalize a cotton swab to evaluate the prevention of S. aureus proliferation. We showed that by using 8 µg of rhamnolipids per swab, the entire bacterial load is eradicated, and just 0.5 µg is sufficient to reduce the growth by 99.99%. Our results strongly indicate the possibility of using this mixture as an additive for wound dressings for chronic wounds. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Pharmaceutics 15 2 700 |
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Article Buonocore, Carmine Giugliano, Rosa Della Sala, Gerardo Palma Esposito, Fortunato Tedesco, Pietro Folliero, Veronica Galdiero, Massimiliano Franci, Gianluigi de Pascale, Donatella Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
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Staphylococcus aureus is a Gram-positive opportunistic human pathogen responsible for severe infections and thousands of deaths annually, mostly due to its multidrug-resistant (MDR) variants. The cell membrane has emerged as a promising new therapeutic target, and lipophilic molecules, such as biosurfactants, are currently being utilized. Herein, we evaluated the antimicrobial activity of a rhamnolipids mixture produced by the Antarctic marine bacterium Pseudomonas gessardii M15. We demonstrated that our mixture has bactericidal activity in the range of 12.5–50 µg/mL against a panel of clinical MDR isolates of S. aureus, and that the mixture eradicated the bacterial population in 30 min at MIC value, and in 5 min after doubling the concentration. We also tested abilities of RLs to interfere with biofilm at different stages and determined that RLs can penetrate biofilm and kill the bacteria at sub-MICs values. The mixture was then used to functionalize a cotton swab to evaluate the prevention of S. aureus proliferation. We showed that by using 8 µg of rhamnolipids per swab, the entire bacterial load is eradicated, and just 0.5 µg is sufficient to reduce the growth by 99.99%. Our results strongly indicate the possibility of using this mixture as an additive for wound dressings for chronic wounds. |
format |
Text |
author |
Buonocore, Carmine Giugliano, Rosa Della Sala, Gerardo Palma Esposito, Fortunato Tedesco, Pietro Folliero, Veronica Galdiero, Massimiliano Franci, Gianluigi de Pascale, Donatella |
author_facet |
Buonocore, Carmine Giugliano, Rosa Della Sala, Gerardo Palma Esposito, Fortunato Tedesco, Pietro Folliero, Veronica Galdiero, Massimiliano Franci, Gianluigi de Pascale, Donatella |
author_sort |
Buonocore, Carmine |
title |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_short |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_full |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_fullStr |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_full_unstemmed |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_sort |
evaluation of antimicrobial properties and potential applications of pseudomonas gessardii m15 rhamnolipids towards multiresistant staphylococcus aureus |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958974/ http://www.ncbi.nlm.nih.gov/pubmed/36840022 https://doi.org/10.3390/pharmaceutics15020700 |
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Antarctic The Antarctic |
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Antarctic The Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
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Pharmaceutics |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958974/ http://www.ncbi.nlm.nih.gov/pubmed/36840022 http://dx.doi.org/10.3390/pharmaceutics15020700 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/pharmaceutics15020700 |
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Pharmaceutics |
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15 |
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700 |
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1766137992000307200 |