Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater
Microbial life in the deep subsurface occupies rock surfaces as attached communities and biofilms. Previously, epilithic Fennoscandian deep subsurface bacterial communities were shown to host genetic potential, especially for heterotrophy and sulfur cycling. Acetate, methane, and methanol link multi...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
Frontiers Media S.A.
2023
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932282/ https://doi.org/10.3389/fmicb.2023.1054084 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:9932282 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:9932282 2023-05-15T16:12:59+02:00 Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater Nuppunen-Puputti, Maija Kietäväinen, Riikka Kukkonen, Ilmo Bomberg, Malin 2023-02-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932282/ https://doi.org/10.3389/fmicb.2023.1054084 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932282/ http://dx.doi.org/10.3389/fmicb.2023.1054084 Copyright © 2023 Nuppunen-Puputti, Kietäväinen, Kukkonen and Bomberg. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Microbiol Microbiology Text 2023 ftpubmed https://doi.org/10.3389/fmicb.2023.1054084 2023-02-19T02:17:22Z Microbial life in the deep subsurface occupies rock surfaces as attached communities and biofilms. Previously, epilithic Fennoscandian deep subsurface bacterial communities were shown to host genetic potential, especially for heterotrophy and sulfur cycling. Acetate, methane, and methanol link multiple biogeochemical pathways and thus represent an important carbon and energy source for microorganisms in the deep subsurface. In this study, we examined further how a short pulse of low-molecular-weight carbon compounds impacts the formation and structure of sessile microbial communities on mica schist surfaces over an incubation period of ∼3.5 years in microcosms containing deep subsurface groundwater from the depth of 500 m, from Outokumpu, Finland. The marker gene copy counts in the water and rock phases were estimated with qPCR, which showed that bacteria dominated the mica schist communities with a relatively high proportion of epilithic sulfate-reducing bacteria in all microcosms. The dominant bacterial phyla in the microcosms were Proteobacteria, Firmicutes, and Actinobacteria, whereas most fungal genera belonged to Ascomycota and Basidiomycota. Dissimilarities between planktic and sessile rock surface microbial communities were observed, and the supplied carbon substrates led to variations in the bacterial community composition. Text Fennoscandian PubMed Central (PMC) Frontiers in Microbiology 14 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbiology |
spellingShingle |
Microbiology Nuppunen-Puputti, Maija Kietäväinen, Riikka Kukkonen, Ilmo Bomberg, Malin Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater |
topic_facet |
Microbiology |
description |
Microbial life in the deep subsurface occupies rock surfaces as attached communities and biofilms. Previously, epilithic Fennoscandian deep subsurface bacterial communities were shown to host genetic potential, especially for heterotrophy and sulfur cycling. Acetate, methane, and methanol link multiple biogeochemical pathways and thus represent an important carbon and energy source for microorganisms in the deep subsurface. In this study, we examined further how a short pulse of low-molecular-weight carbon compounds impacts the formation and structure of sessile microbial communities on mica schist surfaces over an incubation period of ∼3.5 years in microcosms containing deep subsurface groundwater from the depth of 500 m, from Outokumpu, Finland. The marker gene copy counts in the water and rock phases were estimated with qPCR, which showed that bacteria dominated the mica schist communities with a relatively high proportion of epilithic sulfate-reducing bacteria in all microcosms. The dominant bacterial phyla in the microcosms were Proteobacteria, Firmicutes, and Actinobacteria, whereas most fungal genera belonged to Ascomycota and Basidiomycota. Dissimilarities between planktic and sessile rock surface microbial communities were observed, and the supplied carbon substrates led to variations in the bacterial community composition. |
format |
Text |
author |
Nuppunen-Puputti, Maija Kietäväinen, Riikka Kukkonen, Ilmo Bomberg, Malin |
author_facet |
Nuppunen-Puputti, Maija Kietäväinen, Riikka Kukkonen, Ilmo Bomberg, Malin |
author_sort |
Nuppunen-Puputti, Maija |
title |
Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater |
title_short |
Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater |
title_full |
Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater |
title_fullStr |
Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater |
title_full_unstemmed |
Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater |
title_sort |
implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932282/ https://doi.org/10.3389/fmicb.2023.1054084 |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_source |
Front Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932282/ http://dx.doi.org/10.3389/fmicb.2023.1054084 |
op_rights |
Copyright © 2023 Nuppunen-Puputti, Kietäväinen, Kukkonen and Bomberg. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2023.1054084 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
_version_ |
1765998601049210880 |