Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol
In this experimental study, biodiesel was synthesized from the salmon oil using the Lipozyme(®)RM IM (Bagsværd, Denmark) as a biocatalyst. Isoamyl alcohol was used as an acyl acceptor in the transesterification process. The aim of this study is to select the best process conditions, aiming to obtain...
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ftpubmed:oai:pubmedcentral.nih.gov:9919346 2023-05-15T15:32:18+02:00 Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol Gumbytė, Milda Makareviciene, Violeta Sendzikiene, Egle 2023-01-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919346/ http://www.ncbi.nlm.nih.gov/pubmed/36770192 https://doi.org/10.3390/ma16031185 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919346/ http://www.ncbi.nlm.nih.gov/pubmed/36770192 http://dx.doi.org/10.3390/ma16031185 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Materials (Basel) Article Text 2023 ftpubmed https://doi.org/10.3390/ma16031185 2023-02-19T01:42:49Z In this experimental study, biodiesel was synthesized from the salmon oil using the Lipozyme(®)RM IM (Bagsværd, Denmark) as a biocatalyst. Isoamyl alcohol was used as an acyl acceptor in the transesterification process. The aim of this study is to select the best process conditions, aiming to obtain the highest transesterification degree that meets the requirements of the EN 14214 standard. Response surface methodology (RSM) was used for statistical analysis and optimization of process parameters. A four-factor experimental design was modelled by central compositional design (CCD) to investigate the effects of biocatalyst concentration, isoamyl alcohol-to-oil molar ratio, temperature, and duration on transesterification degree. It was determined that the optimal parameters for biodiesel synthesis were the following: an enzyme concentration of 11% (wt. of oil mass); a process temperature of 45 °C; a process duration of 4 h; and an alcohol-to-oil molar ratio of 6:1. The transesterification degree of biodiesel reached 87.23%. The stepwise addition of isoamyl alcohol during the transesterification process further increased the degree of transesterification to 96.5%. Text Atlantic salmon Salmo salar PubMed Central (PMC) Materials 16 3 1185 |
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Article Gumbytė, Milda Makareviciene, Violeta Sendzikiene, Egle Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol |
topic_facet |
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description |
In this experimental study, biodiesel was synthesized from the salmon oil using the Lipozyme(®)RM IM (Bagsværd, Denmark) as a biocatalyst. Isoamyl alcohol was used as an acyl acceptor in the transesterification process. The aim of this study is to select the best process conditions, aiming to obtain the highest transesterification degree that meets the requirements of the EN 14214 standard. Response surface methodology (RSM) was used for statistical analysis and optimization of process parameters. A four-factor experimental design was modelled by central compositional design (CCD) to investigate the effects of biocatalyst concentration, isoamyl alcohol-to-oil molar ratio, temperature, and duration on transesterification degree. It was determined that the optimal parameters for biodiesel synthesis were the following: an enzyme concentration of 11% (wt. of oil mass); a process temperature of 45 °C; a process duration of 4 h; and an alcohol-to-oil molar ratio of 6:1. The transesterification degree of biodiesel reached 87.23%. The stepwise addition of isoamyl alcohol during the transesterification process further increased the degree of transesterification to 96.5%. |
format |
Text |
author |
Gumbytė, Milda Makareviciene, Violeta Sendzikiene, Egle |
author_facet |
Gumbytė, Milda Makareviciene, Violeta Sendzikiene, Egle |
author_sort |
Gumbytė, Milda |
title |
Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol |
title_short |
Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol |
title_full |
Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol |
title_fullStr |
Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol |
title_full_unstemmed |
Enzymatic Transesterification of Atlantic Salmon (Salmo salar) Oil with Isoamyl Alcohol |
title_sort |
enzymatic transesterification of atlantic salmon (salmo salar) oil with isoamyl alcohol |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919346/ http://www.ncbi.nlm.nih.gov/pubmed/36770192 https://doi.org/10.3390/ma16031185 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Materials (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919346/ http://www.ncbi.nlm.nih.gov/pubmed/36770192 http://dx.doi.org/10.3390/ma16031185 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ma16031185 |
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