Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica
Pseudomonas is a widespread genus in various host and environmental niches. Pseudomonas exists even in extremely cold environments such as Antarctica. Pseudomonas antarctica is a psychrophilic bacterium isolated from Antarctica. P. antarctica is also known to produce antimicrobial substances. Althou...
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ftpubmed:oai:pubmedcentral.nih.gov:9864612 2023-05-15T13:44:55+02:00 Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica Kim, Sangha Lee, Changhan 2023-01-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864612/ http://www.ncbi.nlm.nih.gov/pubmed/36677410 https://doi.org/10.3390/microorganisms11010118 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864612/ http://www.ncbi.nlm.nih.gov/pubmed/36677410 http://dx.doi.org/10.3390/microorganisms11010118 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Microorganisms Communication Text 2023 ftpubmed https://doi.org/10.3390/microorganisms11010118 2023-01-29T01:55:56Z Pseudomonas is a widespread genus in various host and environmental niches. Pseudomonas exists even in extremely cold environments such as Antarctica. Pseudomonas antarctica is a psychrophilic bacterium isolated from Antarctica. P. antarctica is also known to produce antimicrobial substances. Although P. antarctica can provide insight into how bacteria have adapted to low temperatures and has significant potential for developing novel antimicrobial substances, progress in genetic and molecular studies has not been achieved. Transposon mutagenesis is a useful tool to screen genes of interest in bacteria. Therefore, we attempted for the first time in P. antarctica to generate transposon insertion mutants using the transfer of a conjugational plasmid encoding a transposon. To increase the yield of transposon insertion mutants, we optimized the methods, in terms of temperature for conjugation, the ratio of donor and recipient during conjugation, and the concentration of antibiotics. Here, we describe the optimized methods to successfully generate transposon insertion mutants in P. antarctica. Text Antarc* Antarctica PubMed Central (PMC) Microorganisms 11 1 118 |
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Communication |
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Communication Kim, Sangha Lee, Changhan Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica |
topic_facet |
Communication |
description |
Pseudomonas is a widespread genus in various host and environmental niches. Pseudomonas exists even in extremely cold environments such as Antarctica. Pseudomonas antarctica is a psychrophilic bacterium isolated from Antarctica. P. antarctica is also known to produce antimicrobial substances. Although P. antarctica can provide insight into how bacteria have adapted to low temperatures and has significant potential for developing novel antimicrobial substances, progress in genetic and molecular studies has not been achieved. Transposon mutagenesis is a useful tool to screen genes of interest in bacteria. Therefore, we attempted for the first time in P. antarctica to generate transposon insertion mutants using the transfer of a conjugational plasmid encoding a transposon. To increase the yield of transposon insertion mutants, we optimized the methods, in terms of temperature for conjugation, the ratio of donor and recipient during conjugation, and the concentration of antibiotics. Here, we describe the optimized methods to successfully generate transposon insertion mutants in P. antarctica. |
format |
Text |
author |
Kim, Sangha Lee, Changhan |
author_facet |
Kim, Sangha Lee, Changhan |
author_sort |
Kim, Sangha |
title |
Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica |
title_short |
Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica |
title_full |
Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica |
title_fullStr |
Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica |
title_full_unstemmed |
Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica |
title_sort |
optimization of transposon mutagenesis methods in pseudomonas antarctica |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864612/ http://www.ncbi.nlm.nih.gov/pubmed/36677410 https://doi.org/10.3390/microorganisms11010118 |
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Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
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Microorganisms |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864612/ http://www.ncbi.nlm.nih.gov/pubmed/36677410 http://dx.doi.org/10.3390/microorganisms11010118 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/microorganisms11010118 |
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Microorganisms |
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11 |
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1 |
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118 |
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