Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate
Antarctic krill is a crucial marine resource containing plenty of high-valued nutrients. However, krill oil as a single product has been developed by the current solvent extraction with high cost. From the perspective of comprehensive utilization of Antarctic krill, this study proposed a novel two-s...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863247/ http://www.ncbi.nlm.nih.gov/pubmed/36662220 https://doi.org/10.3390/md21010047 |
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ftpubmed:oai:pubmedcentral.nih.gov:9863247 2023-05-15T13:44:55+02:00 Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate Teng, Xin-Nan Wang, Shu-Chang Zeb, Liaqat Dong, Yue-Sheng Xiu, Zhi-Long 2023-01-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863247/ http://www.ncbi.nlm.nih.gov/pubmed/36662220 https://doi.org/10.3390/md21010047 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863247/ http://www.ncbi.nlm.nih.gov/pubmed/36662220 http://dx.doi.org/10.3390/md21010047 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2023 ftpubmed https://doi.org/10.3390/md21010047 2023-01-29T01:51:21Z Antarctic krill is a crucial marine resource containing plenty of high-valued nutrients. However, krill oil as a single product has been developed by the current solvent extraction with high cost. From the perspective of comprehensive utilization of Antarctic krill, this study proposed a novel two-step enzymolysis-assisted extraction in attempt to produce value-added oil and enzymolysate simultaneously. After two-step chitinase/protease hydrolysis, the lipid yield increased from 2.09% to 4.18%, reaching 112% of Soxhlet extraction. The method greatly improved the yields of main components while reducing the impurity content without further refining. After optimization, the oil contained 246.05 mg/g of phospholipid, 80.96 mg/g of free eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and 0.82 mg/g of astaxanthin. The by-product enzymolysate was abundant in water-soluble proteins (34.35 mg/g), oligopeptides (13.92 mg/g), amino acids (34.24 mg/g), and carbohydrates (5.79 mg/g), which was a good source of functional nutrients. In addition, both oil and enzymolysate showed high antioxidant capacity. This novel method could simultaneously provide oil and enzymolysate amounting for 58.61% of dried krill. Text Antarc* Antarctic Antarctic Krill PubMed Central (PMC) Antarctic Marine Drugs 21 1 47 |
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Article Teng, Xin-Nan Wang, Shu-Chang Zeb, Liaqat Dong, Yue-Sheng Xiu, Zhi-Long Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
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Article |
description |
Antarctic krill is a crucial marine resource containing plenty of high-valued nutrients. However, krill oil as a single product has been developed by the current solvent extraction with high cost. From the perspective of comprehensive utilization of Antarctic krill, this study proposed a novel two-step enzymolysis-assisted extraction in attempt to produce value-added oil and enzymolysate simultaneously. After two-step chitinase/protease hydrolysis, the lipid yield increased from 2.09% to 4.18%, reaching 112% of Soxhlet extraction. The method greatly improved the yields of main components while reducing the impurity content without further refining. After optimization, the oil contained 246.05 mg/g of phospholipid, 80.96 mg/g of free eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and 0.82 mg/g of astaxanthin. The by-product enzymolysate was abundant in water-soluble proteins (34.35 mg/g), oligopeptides (13.92 mg/g), amino acids (34.24 mg/g), and carbohydrates (5.79 mg/g), which was a good source of functional nutrients. In addition, both oil and enzymolysate showed high antioxidant capacity. This novel method could simultaneously provide oil and enzymolysate amounting for 58.61% of dried krill. |
format |
Text |
author |
Teng, Xin-Nan Wang, Shu-Chang Zeb, Liaqat Dong, Yue-Sheng Xiu, Zhi-Long |
author_facet |
Teng, Xin-Nan Wang, Shu-Chang Zeb, Liaqat Dong, Yue-Sheng Xiu, Zhi-Long |
author_sort |
Teng, Xin-Nan |
title |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_short |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_full |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_fullStr |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_full_unstemmed |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_sort |
two-step enzymolysis of antarctic krill for simultaneous preparation of value-added oil and enzymolysate |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863247/ http://www.ncbi.nlm.nih.gov/pubmed/36662220 https://doi.org/10.3390/md21010047 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863247/ http://www.ncbi.nlm.nih.gov/pubmed/36662220 http://dx.doi.org/10.3390/md21010047 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md21010047 |
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Marine Drugs |
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21 |
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47 |
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1766208570935738368 |